Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for protein-polyose-polylactic acid polycaprolactone vascular stent

A technology of polycaprolactone and vascular stents, which is applied in the field of preparation of tissue engineering vascular stents, and achieves the effects of simple and easy preparation methods, promotion of repair and regeneration, and wide application prospects

Inactive Publication Date: 2013-06-12
DONGHUA UNIV
View PDF5 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the application of collagen, chitosan and P(LLA-CL) composite nanofiber electrospinning technology in small-caliber vascular tissue engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for protein-polyose-polylactic acid polycaprolactone vascular stent
  • Preparation method for protein-polyose-polylactic acid polycaprolactone vascular stent
  • Preparation method for protein-polyose-polylactic acid polycaprolactone vascular stent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 0.8g of collagen, chitosan and P(LLA-CL) with an electronic balance; dissolve 0.8g of collagen in 10ml hexafluoroisopropanol, and magnetically stir until completely dissolved; Dissolve chitosan in 10ml of hexafluoroisopropanol / trifluoroacetic acid (V / V, 9 / 1) mixed solvent, stir magnetically until completely dissolved; dissolve 0.8g of P(LLA-CL) in 10ml of hexafluoroisopropanol In propanol, magnetically stir until completely dissolved; after the three solutions are completely dissolved, take 2ml of collagen solution, 0.5ml of chitosan solution, and 7.5ml of P(LLA-CL) solution and mix them in a volume ratio of 20:5:75. Stir and mix evenly with magnetic force to obtain 10 ml of a mixed spinning solution with a total concentration of 8%, and the mass ratio of the solute collagen-chitosan-P(LLA-CL) is 20:5:75. Inhale the mixed solution into the syringe and control the advance speed of the micro-injection pump to be 1ml / h. Select a No. 7 stainless steel needle and conne...

Embodiment 2

[0031] Weigh 0.8g of collagen, chitosan and P(LLA-CL) with an electronic balance; dissolve 0.8g of collagen in 10ml hexafluoroisopropanol, and magnetically stir until completely dissolved; Dissolve chitosan in 10ml of hexafluoroisopropanol / trifluoroacetic acid (V / V, 9 / 1) mixed solvent, stir magnetically until completely dissolved; dissolve 0.8g of P(LLA-CL) in 10ml of hexafluoroisopropanol In propanol, magnetically stir until completely dissolved; after the three solutions are completely dissolved, take 4ml of collagen solution, 1ml of chitosan solution, and 5ml of P(LLA-CL) solution in a volume ratio of 40:10:50, and magnetically stir Mix evenly to obtain 10 ml of mixed spinning solution with a total concentration of 8%, and the mass ratio of solute collagen-chitosan-P(LLA-CL) is 40:10:50. Inhale the mixed solution into the syringe and control the advance speed of the micro-injection pump to be 1ml / h. Select a No. 7 stainless steel needle and connect it to a 14KV high voltage...

Embodiment 3

[0033]Weigh 0.8g of collagen, chitosan and P(LLA-CL) with an electronic balance; dissolve 0.8g of collagen in 10ml of hexafluoroisopropanol, and magnetically stir until completely dissolved; Dissolve chitosan in 10ml of hexafluoroisopropanol / trifluoroacetic acid (V / V, 9 / 1) mixed solvent, and magnetically stir until completely dissolved; dissolve 0.8g of P(LLA-CL) in 10ml of hexafluoroisopropanol In propanol, magnetically stir until completely dissolved; after the three solutions are completely dissolved, take 6ml of collagen solution, 1.5ml of chitosan solution, and 2.5ml of P(LLA-CL) solution and mix them in a volume ratio of 60:15:25. Magnetic stirring and mixing were performed uniformly to obtain 10 ml of a mixed spinning solution with a total concentration of 8%, and the mass ratio of the solute collagen-chitosan-P(LLA-CL) was 60:15:25. Inhale the mixed solution into the syringe and control the advance speed of the micro-injection pump to be 1ml / h. Select a No. 7 stainless...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method for a protein-polyose-polylactic acid polycaprolactone vascular stent. The method comprises the following steps: (1) completely dissolving chitosan into a hexafluoroisopropanol and trifluoroacetic acid mixed solution to obtain a chitosan solution; (2) completely dissolving collagen into hexafluoroisopropanol to obtain a collagen solution; (3) completely dissolving polylactic acid polycaprolactone into hexafluoroisopropanol to obtain a polylactic acid polycaprolactone solution; and (4) mixing and stirring the chitosan solution, the collagen solution and the polylactic acid polycaprolactone solution to obtain a protein-polyose-polylactic acid polycaprolactone spinning solution, and then achieving static spinning to obtain the protein-polyose-polylactic acid polycaprolactone vascular stent. The prepparation materials have superior biocompatibility and mechanical property; and the designed multi-component composite biological material has a broad application prospect, and the preparation method is simple and feasible and is mainly used in small-caliber vascular tissue engineering.

Description

technical field [0001] The invention belongs to the field of preparation of tissue engineering vascular stents, in particular to a preparation method of protein-polysaccharide-polylactic acid polycaprolactone vascular stents. Background technique [0002] With the increasing severity of clinical tissue defects and organ failure, ordinary repairs and even organ transplantation have been difficult to meet the needs of patients, especially cardiovascular disease has become one of the great threats to human health due to its high incidence (Canver CC. Conduit Options in Coronary Artery Bypass Surgery. CHEST Journal. 1995;108:1150-5.). Traditional surgical methods such as coronary artery bypass grafting are difficult to be widely used due to the limitation of donor shortage, while tissue engineering methods have shown important application prospects (Langer R, Vacanti J. Tissue engineering. Science. 1993; 260: 920 -6.). At present, a large number of natural materials and polyme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/4382D04H1/728A61L27/26
Inventor 莫秀梅吴桐尹岸林
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com