Method for evaluating animal individual nutriture by metabonomics

A technology for animals and animal samples, applied in scientific instruments, measuring devices, instruments, etc., can solve problems such as establishing mathematical models without metabolomics data, achieve the effect of optimizing formula and improving production efficiency

Active Publication Date: 2013-06-12
NINGBO TECH BANK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the use of metabolomics data to establish mathematical models and determine biomarkers for quantitative evaluation of animal energy and protein intake

Method used

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  • Method for evaluating animal individual nutriture by metabonomics
  • Method for evaluating animal individual nutriture by metabonomics
  • Method for evaluating animal individual nutriture by metabonomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Diets with different energy and protein content carry out the establishment of data model for animal energy protein intake evaluation

[0055] 1. Materials and methods

[0056] 1. Test animals

[0057] 50 healthy Duroc×Landrace×Large White ternary growing pigs were selected for the experiment, weighing 21.7±0.5kg. According to the principle of similar body weight and similar genetic basis, they were randomly divided into 5 groups, 10 in each group, half male and half female, and raised in a single pen.

[0058] 2. Test diet

[0059] The experiment was divided into 5 treatments, namely control group (normal energy protein level diet MEMP), high energy high protein group (HEHP), low energy low protein group (LELP), high energy low protein group (HELP) and low energy high protein group (LEHP ). The nutrient level of the diet in the control group was designed according to the recommended values ​​in the "Pig Feeding Standards" (2004), and the other diets were...

Embodiment 2

[0077] Example 2. Evaluation of animal protein and energy intake by metabolomics technology based on liquid chromatography-mass spectrometry

[0078] 1. Data collection and processing

[0079] 1. Sample handling

[0080] The 50 plasma samples of Example 1 were taken out from the -80°C refrigerator and thawed on ice. 100 μL of plasma was taken and processed as follows: adding 400 μL of extract solution (mixed with methanol and acetonitrile at a volume ratio of 1:1), vortexed for 5 min, and then centrifuged at 4°C and 13,000 rpm for 10 min. Carefully pipette 200 μL of the supernatant into a new centrifuge tube, dry it with nitrogen gas at room temperature, and redissolve in 200 μL of the initial organic phase solution (acetonitrile and water mixed at a volume ratio of 5:95). After shaking for 5 s, centrifuge at 4°C and 13,000 rpm for 10 min, take 2 μL of the supernatant and put it into a sample bottle for detection by high-performance liquid chromatography-quadrupole-time-of-f...

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Abstract

The invention discloses a method for evaluating animal individual nutriture by metabonomics. The method is characterized in that through building a regression model of crude protein content and digestible energy content of daily ration of animals and types and content of metabolites in an animal body fluid, protein and energy levels of the daily ration of the animals can be forecasted. The method can determine if protein and energy nutrient levels of daily ration of a growing pig are different from the normal nutrient levels, can determine a nutrition type, and has an accuracy rate of 100%. The method can find 11 biomarkers related to a protein level of daily ration, 9 biomarkers related to an energy level of the daily ration, and 5 biomarkers related to the protein level and the energy level. The method has important theoretical and practical significance for evaluating pig nutriture, optimizing a formula and improving pig production efficiency.

Description

technical field [0001] The invention relates to a method for evaluating the nutritional status of individual animals, in particular to a method for evaluating the nutritional status of individual animals using metabolomics technology. Background technique [0002] With the increase of food pressure, the shortage of feed raw materials and the enhancement of human awareness of environmental protection, it will be of great significance to improve feed conversion rate and utilization rate, reduce N and P emissions, and realize a healthy, efficient, high-yield, ecological and safe farming model. . At present, animal nutrition research is becoming more and more refined, and the precise determination of nutrient requirements to regulate animal growth has become a research hotspot at home and abroad. In order to overcome the apparent and static defects of the indicators in the current standards, in order to help farms optimize the feeding mode, the feeding standards must be changed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王军军林刚王泰极戴兆来李溱王春林李德发
Owner NINGBO TECH BANK
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