Method for evaluating animal individual nutriture by metabonomics
A technology for animals and animal samples, applied in scientific instruments, measuring devices, instruments, etc., can solve problems such as establishing mathematical models without metabolomics data, achieve the effect of optimizing formula and improving production efficiency
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Embodiment 1
[0054] Example 1. Diets with different energy and protein content carry out the establishment of data model for animal energy protein intake evaluation
[0055] 1. Materials and methods
[0056] 1. Test animals
[0057] 50 healthy Duroc×Landrace×Large White ternary growing pigs were selected for the experiment, weighing 21.7±0.5kg. According to the principle of similar body weight and similar genetic basis, they were randomly divided into 5 groups, 10 in each group, half male and half female, and raised in a single pen.
[0058] 2. Test diet
[0059] The experiment was divided into 5 treatments, namely control group (normal energy protein level diet MEMP), high energy high protein group (HEHP), low energy low protein group (LELP), high energy low protein group (HELP) and low energy high protein group (LEHP ). The nutrient level of the diet in the control group was designed according to the recommended values in the "Pig Feeding Standards" (2004), and the other diets were...
Embodiment 2
[0077] Example 2. Evaluation of animal protein and energy intake by metabolomics technology based on liquid chromatography-mass spectrometry
[0078] 1. Data collection and processing
[0079] 1. Sample handling
[0080] The 50 plasma samples of Example 1 were taken out from the -80°C refrigerator and thawed on ice. 100 μL of plasma was taken and processed as follows: adding 400 μL of extract solution (mixed with methanol and acetonitrile at a volume ratio of 1:1), vortexed for 5 min, and then centrifuged at 4°C and 13,000 rpm for 10 min. Carefully pipette 200 μL of the supernatant into a new centrifuge tube, dry it with nitrogen gas at room temperature, and redissolve in 200 μL of the initial organic phase solution (acetonitrile and water mixed at a volume ratio of 5:95). After shaking for 5 s, centrifuge at 4°C and 13,000 rpm for 10 min, take 2 μL of the supernatant and put it into a sample bottle for detection by high-performance liquid chromatography-quadrupole-time-of-f...
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