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Method for seed disinfection in Allium fistulosum L. tissue culture

A technology of tissue culture and disinfection method, which is applied in horticultural methods, botany equipment and methods, horticulture, etc. It can solve the problems that there are few reports on the disinfection method of scallion seeds, and achieve low pollution rate, high germination rate, and high seedling growth rate effect

Inactive Publication Date: 2013-06-19
XIAN REJE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, various sterilization methods have been reported (Yang Lan et al., 2008; John H et al., 1985; R.L.M.Pierik, 1987; Trevor A , 1981; Wu Lianghuan et al., 1997, 1999), but there are few reports on the method of disinfection of scallion seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Using Zhangqiu scallion seeds as the material, first rinse the scallion seeds with tap water for 30 minutes, and add 1-2 drops of Tween-20 to the triangular flask when rinsing with tap water; then place it on an ultra-clean bench and rinse with 75% alcohol for 30 seconds; Disinfect with 2% sodium hypochlorite for 40 minutes; rinse 3-5 times with sterile water added with Tween-20, and finally rinse once with sterile water to complete the disinfection.

Embodiment 2

[0046] Using Zhangqiu scallion seeds as the material, first rinse the scallion seeds with tap water for 20 minutes, and add 1-2 drops of Tween-20 to the triangular flask when rinsing with tap water; then place it on an ultra-clean bench and rinse with 75% alcohol for 60 seconds; Disinfect with 2% sodium hypochlorite for 30 minutes; rinse 3-5 times with sterile water added with Tween-20, and finally rinse once with sterile water to complete the disinfection.

Embodiment 3

[0048] Using Zhangqiu scallion seeds as materials, first rinse the scallion seeds with tap water for 30 minutes, and add 2 drops of Tween-20 to the triangular flask when rinsing with tap water; % sodium hypochlorite for 50 minutes; rinse 3-5 times with sterile water added with Tween-20, and finally rinse once with sterile water to complete the disinfection.

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PUM

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Abstract

The invention discloses a method for seed disinfection in Allium fistulosum L. tissue culture. The method includes the steps of: 1) flushing Allium fistulosum L. seeds with tap water, and at the same time, adding tween-20 into a triangular flask; 2) placing the seeds on an ultra-clean workbench and conducting rinsing with 75% alcohol; 3) carrying out disinfection with 2% sodium hypochlorite; and 4) performing flushing for 3-5 times with sterile water added with tween-20, and finally conducting flushing once with sterile water, as the optimal. The Allium fistulosum L. seeds disinfected by the method provided in the invention have a high germination rate and a low contamination rate, thus ensuring a high seedling rate.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a method for disinfecting seeds in tissue culture of scallions. Background technique [0002] green onions ( Allium fistulosum L.) is a biennial herb of the Allium genus of Liliaceae, rich in calcium, phosphorus, and vitamins. It is not only delicious, but also has medical and health care effects. According to FAO statistics, in 2009 my country's cultivated green onion area reached 26,560 hectares, ranking first in the world. [0003] In recent years, with the rapid development of molecular biotechnology and the increasing popularity of genetic engineering, the use of transgenic plants to produce drugs, vaccines and biological agents has increasingly shown broad application prospects and potential economic benefits. In the study of transgenic plants, the screening and cultivation of sterile seedlings is the key to the success of the experiment, and sterile seedlings a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 步怀宇
Owner XIAN REJE BIOLOGICAL TECH
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