Lentinula edodes strain suitable for industrialized cultivation and industrialized cultivation method of lentinula edodes strain

A cultivation method and technology of shiitake mushrooms, which are applied in the field of shiitake mushroom strains and their factory cultivation, can solve the problems of reduced mushroom quality, unstable output, high pollution rate of shiitake mushroom sticks, and achieve fast budding of mushroom sticks, small individual differences, Neat fruiting effect

Active Publication Date: 2019-05-17
SHANDONG QIHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a kind of shiitake mushroom strain suitable for factory cultivation, which solves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The strain "Qihe 17" of the present invention and the parent strain "Qihe 2" were used as test materials, and PDA medium was used. The above two strains were respectively inoculated onto culture dishes for confrontation culture (group 7), cultured at a constant temperature at 25°C, and the colonies of the two strains in the culture dish were observed after 20 days. The antagonism in each culture medium is shown in Table 1, wherein, " +++" indicates that the antagonism is very obvious, "++" indicates that the antagonism is more obvious, and "+" indicates that there seems to be antagonism.

[0042] Antagonism situation in each culture dish of table 1

[0043] serial number

[0044] It can be seen from Table 1 that the "Qihe 17" strain obtained by spore hybridization with "Qihe 2" as the starting strain is a completely different new variety of shiitake mushroom.

Embodiment 2

[0046] (1) Prepare strains:

[0047] Erlenmeyer flask liquid strain: cut the mycelial culture into 4-5mm square pieces, take 4-10 pieces and put them in the sterilized Erlenmeyer flask liquid culture medium, stir evenly, and cultivate at a constant temperature at 25°C. After 7-8 days, the shiitake mushroom balls grow well and are used as secondary species. The preparation method of Erlenmeyer flask liquid medium is as follows: add 100-500g of potatoes per liter of water to boil and filter, add glucose 10-30g, peptone 1-5g, potassium dihydrogen phosphate 1-4g, anhydrous magnesium sulfate 0.5- 2g, mix well.

[0048] Fermentation tank liquid strain: put the liquid strain in the Erlenmeyer flask into the fermenter liquid culture medium under aseptic operation, keep the tank pressure at 0.01-0.05MPa, and cultivate at a constant temperature of 25°C. After 7-9 days, the fermentation liquid will become thick used when. The preparation method of the fermenter liquid medium formula i...

Embodiment 3

[0062] (1) Strain preparation:

[0063] Mother species: Cut the mycelium culture to a size of 4mm×4mm, place the mycelium culture in a sterilized PDA test tube, and cultivate it at a constant temperature at 25°C, and use it as a mother species after the mycelia of the shiitake mushrooms are overgrown.

[0064] Original species: Soak 0.7cm×0.7cm thick and 15cm poplar wood strips in water, dip in corn paste and bran, put them in polyethylene or polypropylene plastic bags, and put 40-70 sticks in each bag, and press high pressure Sterilize at 121°C for 2 hours, after cooling, inoculate the mother seed under aseptic conditions, and cultivate at 25°C until the mycelia cover the whole bag to obtain the original seed wood strips.

[0065] Production species: Mix 3-6mm sieved hard broad-leaved wood chips and bran at a mass ratio of 4:1, add water to adjust the water content to 58-60%, put it into a 17×35cm polypropylene plastic bag, seal it, and sterilize at 121°C After 2 hours, afte...

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Abstract

The invention relates to the technical field of industrialized fungus cultivation, in particular to a lentinula edodes strain suitable for industrialized cultivation and an industrialized cultivationmethod of the lentinula edodes strain. The lentinula edodes strain suitable for the industrialized cultivation is collected in CGMCC (China General Microbiological Culture Collection Center) with thecollection number of CGMCC 16919 on December 25th, 2018, and the name is Qihe 17. The industrialized cultivation method of the lentinula edodes strain suitable for industrialized cultivation comprisessteps as follows: preparation of strains, production of fungus sticks or tied fungus bags, inoculation, preculture, culture and fruiting management. The problems of high contamination rate, unstableyield and reduction of fruiting quality in traditional lentinula edodes stick production are solved, biotransformation rate of two crops of lentinula edodes is 70%-83%, and 98% or more of lentinula edodes sticks can realize normal budding and fruiting, fruiting is neat, the lentinula edodes is in middle-large size, and flower shape is round and normal.

Description

technical field [0001] The invention relates to the technical field of mushroom factory cultivation, in particular to a shiitake mushroom strain suitable for factory cultivation and a factory cultivation method thereof. Background technique [0002] my country is the largest country in the production of edible fungi. In 2017, the total output of shiitake mushrooms in my country was more than 9 million tons, and the annual output of shiitake mushrooms has increased year by year. At the same time, with the development of shiitake mushroom sauce and other processed shiitake mushrooms, new vitality has been added to the vigorous rise of the shiitake mushroom industry. However, the production mode of most of the main production areas of shiitake mushrooms in my country is backward, and the adaptability and quality of the strains are prominent, resulting in frequent problems such as batch pollution of traditional shiitake mushroom cultivation, strain degradation, and low quality a...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/00A01G18/20
Inventor 张振宇李洁董海英高霞黄雪郝宇晴
Owner SHANDONG QIHE BIOTECH
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