Preparation method of aquaporin AqpZ
An aquaporin and fusion protein technology, applied in the field of aquaporin AqpZ, can solve the problems of difficult to meet the structural research and prepare a water recovery device, and achieve the effect of avoiding toxicity
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[0032] The preparation method of aquaporin AqpZ of the present invention comprises the following steps:
[0033] 1. Amplification of E. coli aquaporin AqpZ-HIS: Two pairs of primers were designed according to the E. coli aquaporin gene sequence on NCBI. Eight HIS affinity tags were artificially added to the 3' end of the AqpZ gene by designing primers. The oligonucleotide sequence of the upstream primer of the first primer is shown in SEQ ID No.1, the oligonucleotide sequence of the downstream primer of the first primer is shown in SEQ ID No.2, and the oligonucleotide sequence of the upstream primer of the second primer is shown in SEQ ID No.2. The nucleotide sequence is shown in SEQ ID No.3, and the oligonucleotide sequence of the downstream primer of the second primer is shown in SEQ ID No.4. According to the characteristics of the multiple cloning sites of the two vectors pMAL-p2 and pMAL-c5e, add the upstream and downstream of the first primer respectively Bam HI and ...
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