Portable electrochemical detection test strip and preparation method thereof

A detection test paper, electrochemical technology, applied in the field of biosensing, can solve the problems of inaccurate blood glucose test test paper results, inaccurate measurement, etc., to achieve the effect of reduced resistance value, high precision, and improved relative activity

Inactive Publication Date: 2013-06-26
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of detection time and the inability to rule out the influence of various interference factors, this kind of blood glucose test strip has problems such as inaccurate results and inaccurate measurement.

Method used

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  • Portable electrochemical detection test strip and preparation method thereof
  • Portable electrochemical detection test strip and preparation method thereof
  • Portable electrochemical detection test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] refer to figure 1 , a portable electrochemical detection test strip, comprising an insulating base 1, on which a working electrode 2 and an L-shaped reference electrode 4 are printed, and a reaction zone 6 composed of an enzyme layer is sprayed on the working electrode 2 and the reference electrode on electrode 4.

[0043] The electrode is a carbon electrode prepared from conductive carbon paste.

[0044] The enzyme layer contains horseradish catalase, benzoquinone, polyethylene glycol and acetic acid buffer solution with a pH value of 7.4, and is used to detect the presence or concentration of hydrogen peroxide in the sample. The usage ratio of each component adopts any known and applicable ratio in the field of electrochemical test strips.

[0045] see Figure 7 , a test strip tester interface used in conjunction with the electrochemical test strip, including a base and a conductive device 12 in two parts. The base includes two parts, an upper end 10 and a lower e...

Embodiment 2

[0055] refer to figure 2 , a portable electrochemical detection test strip, including an insulating substrate 1, printed with working electrodes 2, 3 and a T-shaped reference electrode 4 on the insulating substrate, and an insulating layer 5 covering part of the working electrode and part of the reference electrode , the reaction zone 6 composed of an enzyme layer and a non-enzyme layer is sprayed on the working electrodes 2, 3 and the reference electrode 4.

[0056] The electrode is a carbon electrode prepared from a conductive carbon paste containing 3% by weight of carbon nanotubes.

[0057] The enzyme layer is sprayed on the working electrode 2 and contains glucose oxidase, potassium ferricyanide, polyethylene glycol, hydroxyethyl cellulose and sodium succinate buffer solution with a pH value of 5.0. Used to detect the glucose concentration in the sample.

[0058] The non-enzyme layer is sprayed on the working electrode 3 and the reference electrode 4, and contains pota...

Embodiment 3

[0071] refer to image 3 , a multifunctional portable electrochemical detection test strip, including an insulating substrate 1, printed with working electrodes 2, 3 and a T-shaped reference electrode 4 on the insulating substrate, and an insulating layer 5 covering part of the working electrode and part of the reference electrode. On the electrode, the reaction zone 6 formed by the enzyme layer is printed on the working electrodes 2, 3 and the reference electrode 4 by screen printing, and the double-sided adhesive tape is pasted on the edge of the reaction zone 6 to form the adhesive layer 7, and the upper part is covered with a hydrophilic The membrane 8 is surrounded by the enzyme layer 6, the adhesive layer 7 and the hydrophilic membrane 8 to form a micro-injection cavity 9.

[0072] The thickness of the adhesive layer 7 is 0.1mm, and the distance between the two adhesive layers is 1mm; the volume of the sample injection cavity is not greater than 1.5uL.

[0073] The elec...

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Abstract

The invention belongs to the technical field of biosensing, and relates to a multifunctional portable electrochemical detection test strip and a preparation method thereof. The electrochemical detection test strip comprises an insulating substrate, at least one working electrode and a reference electrode which are arranged on the insulating substrate, and reaction areas which are arranged on the working electrode and the reference electrode, and the electrochemical detection test strip is characterized in that the reference electrode is designed to be of a structure to form an electricity loop with an electrochemical test strip detector after being inserted into the electrochemical test strip detector so that the electrochemical test strip detector is turned on in response to the insertion of the electrochemical detection test strip. The preparation method of the test strip comprises the steps of: arranging the working electrode and the reference electrode on the insulating substrate, and arranging the reaction areas on the electrodes. An interface for the electrochemical test strip comprises a conducting copper belt respectively contacting the working electrode and the reference electrode. The test strip disclosed by the invention allows the turning-on by inserting the strip, the measurement is preformed after a sample is filled and the non-specific interference of the sample is eliminated, the result is accurate and the measurement is precise.

Description

technical field [0001] The invention belongs to the technical field of biosensing, relates to a test strip, in particular to a multifunctional portable electrochemical detection test strip and a preparation method thereof. Background technique [0002] At present, the portable test strips used for electrochemical detection are mainly for testing blood sugar, which are composed of a substrate, an enzyme layer and a siphon tank, and the substrate is generally printed or sprayed with a working electrode, a reference electrode, an electrical circuit and used to communicate with the detector. connected wires. Many blood glucose meters collect data within a limited time after blood sample injection. When the blood volume is small or the sample injection is incomplete, but the time reaches the scheduled time for blood glucose meter data collection, the instrument will automatically perform the test, so the blood sample is not full. The test will cause deviations in the test result...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 彭勇李文韬
Owner 复星诊断科技(长沙)有限公司
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