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Baccharis dracunculifolia propolis quality control method

A quality control method and technology of Bacchus chrysanthemum, which are applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of uneven quality of Bacchus type propolis, lack of quality evaluation methods of Bacchus type propolis, and the like, and achieve sensitivity. High, high feasibility, good separation effect

Active Publication Date: 2013-06-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of reasonable quality evaluation methods of Dionysus-type propolis, the quality of Dionysus-type propolis from different companies or even different batches of the same company is uneven.

Method used

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  • Baccharis dracunculifolia propolis quality control method
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  • Baccharis dracunculifolia propolis quality control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Accurately weigh 10 mg of the standard product of atipirin C, and dilute it to 10 mL with methanol to prepare the standard stock solution of atipirin C; absorb a certain amount of standard stock solution of atipirin C as needed, and dilute it with methanol Dilute to volume and prepare standard working solutions of 0.1, 02, 0.3, 0.4, 0.5, 0.6 mg / mL. They were filtered through microporous membranes and analyzed by HPLC. Draw the standard curve of atipirin C.

[0026] Take a certain amount of test samples for freezing, pulverization and mixing, take 0.25 g Dionysus propolis respectively, the ratio of solid to liquid is 1:15, ultrasonic for 30 min, extract three times, filter and rotary evaporate to obtain the extract, and then use Pure methanol was ultrasonically dissolved to a volume of 50 mL, filtered through a microporous membrane, and determined by HPLC.

[0027] The conditions of the liquid chromatograph are: the flow rate is 1.0 mL / min, the detection wavelength is ...

Embodiment 2

[0040] Take 0.3 mg / mL standard working solution, filter it with a microporous membrane, and analyze it by HPLC.

[0041] A certain amount of Shandong poplar-type propolis and poplar gum were frozen, crushed and mixed, and 10 g were taken respectively, according to the ratio of solid to liquid 1:15, ultrasonically extracted for 3 times, filtered, and rotary evaporated to obtain the extract. Take 0.25 g of the extract respectively, dissolve it in ultrasonic with pure methanol, filter it through a microporous membrane, and measure it by HPLC.

[0042] The conditions of the liquid chromatograph are: the flow rate is 1.0mL / min, the detection wavelength is 310 nm, the injection volume is 2 μL, the chromatographic column is Sepax HP-C18 (4.6×150mm, 5 μm) or equivalent chromatographic column, and the column temperature is is 30°C. The mobile phase is pure methanol and 1% acetic acid aqueous solution with a volume ratio of 1% for gradient elution by volume ratio. The elution program i...

Embodiment 3

[0044] Take 0.3 mg / mL standard working solution, filter it through a microporous membrane, and analyze it by HPLC.

[0045] A certain amount of Australian Eucalyptus propolis and Taiwan Macaranga spp. were frozen and pulverized, 10 g were taken respectively, and the material-to-liquid ratio was 1:15, ultrasonicated for 30 min, extracted three times, filtered, and rotary evaporated to obtain extracts. Take 0.25 g of the extract respectively, dissolve it in ultrasonic with pure methanol, filter it through a microporous membrane, and measure it by HPLC.

[0046] The conditions of the liquid chromatograph are: the flow rate is 1.0mL / min, the detection wavelength is 310 nm, the injection volume is 2 μL, the chromatographic column is Sepax HP-C18 (4.6×150mm, 5 μm) or equivalent chromatographic column, and the column temperature is is 30°C. The mobile phase is pure methanol and 1% acetic acid aqueous solution with a volume ratio of 1% for gradient elution by volume ratio. The elutio...

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Abstract

The invention provides a Baccharis dracunculifolia propolis quality control method. The method comprises the following steps: refrigerating a test sample, crushing, uniformly mixing, carrying out ultrasonic extraction with ethanol, filtering, concentrating to obtain an extract, dissolving the extract by ethanol, filtering through a micro-porous film, determining through a high performance liquid chromatography determination process, determining that whether there is Baccharis dracunculifolia propolis or not through the existence or inexistence of artepillin C, and identifying the good and bad quality standard of the Baccharis dracunculifolia propolis through the content of artepillin C. The detection method for distinguishing the Baccharis dracunculifolia propolis and other types of propolis is established for the first time, and a case that artepillin C is a Baccharis dracunculifolia propolis discriminating index and also is a Baccharis dracunculifolia propolis quality evaluation index is provided. The method has the advantages of reasonable design, simple operation, good separating effect, high sensitivity and strong feasibility, and solves an important technical problem in the discrimination and quality control of the Baccharis dracunculifolia propolis.

Description

technical field [0001] The invention belongs to the field of propolis quality control, and relates to a quality control method of Dionysus type propolis, which is used for identifying Dionysus type propolis and evaluating the quality of Dionysus type propolis. Background technique [0002] Propolis is a fragrant solid made by bees collecting the resin secreted by plant leaf buds or bark cracks and mixed with mandibular gland secretions and beeswax. Propolis has various physiological and pharmacological activities such as antibacterial, anti-protozoa, anti-virus, anti-oxidation, anti-tumor, regulating blood lipid and blood sugar, and improving immunity. However, the chemical composition and pharmacological activity of propolis vary greatly depending on the geographical location where propolis is collected. [0003] According to the plant source of propolis, propolis in the world can be divided into five types: poplar type, Dionysus type, Crucy type, Macaranga type and Medite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张翠平胡福良黄帅
Owner ZHEJIANG UNIV
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