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Method for on-line synthesizing saccharose-6-laurate by lipase catalysis

A technology of laurate and lipase, applied in biochemical equipment and methods, biochemical instruments, biochemical cleaning devices, etc., can solve the problems of long reaction time, difficult separation, low conversion rate and selectivity, and achieve high reaction Effects of selectivity, shortened reaction time, and high conversion

Active Publication Date: 2013-07-03
ZHEJIANG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method often requires a long reaction time (24h), and the conversion rate and selectivity of the reaction are not high, and subsequent separation is difficult.

Method used

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  • Method for on-line synthesizing saccharose-6-laurate by lipase catalysis
  • Method for on-line synthesizing saccharose-6-laurate by lipase catalysis
  • Method for on-line synthesizing saccharose-6-laurate by lipase catalysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the synthesis of sucrose-6-laurate

[0028] refer to figure 1 , sucrose (0.4mmol) was dissolved in 10mL (tert-amyl alcohol: DMSO = 4:1 (v / v)) mixed solvent, vinyl laurate (4.4mmol) was dissolved in 10mL tert-amyl alcohol, and then packed separately Prepare in a 10mL syringe. 0.87g of lipase Lipozyme TLIM was evenly filled in the reaction channel, and driven by the PHD2000 syringe pump, the two reaction solutions were mixed with 10.4μLmin -1The flow rate enters the reaction channel through the "Y" joint to carry out the acylation reaction. The temperature of the reactor is controlled at 52°C through a water bath thermostat, and the reaction solution flows continuously in the reaction channel for 30 minutes. The reaction solution is collected online by the product collector, and the pressure is reduced The solvent was distilled off, and the column was wet-packed with 200-300 mesh silica gel. The eluent was chloroform:methanol=10:1, the column height was 35...

Embodiment 2-5

[0032] Change the temperature of the microfluidic channel reactor, others are the same as in Example 1, and the reaction results are as shown in Table 1:

[0033] Table 1: Effect of Temperature on Reaction

[0034]

[0035] [a]: The conversion rate was determined by HPLC; [b]: The content of sucrose-6-laurate in the total sucrose ester product

[0036] The results in Table 1 show that when the flow rate is 10.4μLmin -1 , when the reaction time is 30min, the conversion rate increases obviously with the increase of the reaction temperature. When the reaction temperature reaches 52°C, the conversion rate and selectivity of the reaction are the best. The decrease in enzyme activity leads to a decrease in the conversion rate and selectivity of the reaction, so the optimal reaction temperature of sucrose-6-laurate in the microfluidic channel reactor is 52°C.

Embodiment 6-9

[0038] Change the substrate molar ratio of vinyl laurate and sucrose in the microfluidic microchannel reactor to be 8: 1 (Example 6), 9: 1 (Example 7), 10: 1 (Example 8), 12: 1 (embodiment 9), other are with embodiment 1, and the result is as shown in table 2.

[0039] Table 2: Effect of sucrose to vinyl laurate substrate ratio on the reaction

[0040]

[0041]

[0042] [a]: conversion rate is measured by HPLC; [b]: the result of the content table 2 of sucrose-6-laurate in the total sucrose ester product shows that, along with the increase of reactant vinyl laurate, the conversion rate of reaction also increases With the increase, when the substrate ratio is 11:1, the conversion and selectivity of the reaction are optimal. Now if continue to increase the consumption of reactant vinyl laurate, will cause the selectivity of reaction to reduce, thereby, the optimal substrate ratio of this reaction is 11:1.

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Abstract

The invention provides a method for on-line synthesizing saccharose-6-laurate by lipase catalysis, comprising: using saccharose and vinyl laurate, with a mol ratio of 1:8-12, as raw materials, using 0.5-10g of Lipozyme TLIM as a catalyst, and using a mixed solvent of tertiary amyl alcohol and DMSO as a reaction solvent, uniformly filling Lipozyme TLIM in a reaction channel of a microfluidic channel reactor, wherein the internal diameter of the reaction channel of the microfluidic channel reactor is 0.8-2.4mm, and the length of the reaction channel is 0.5-1.0m; continuously introducing the raw materials and the reaction solvent into the reaction channel to perform acylation reaction under 40-55 DEG C for 20-35min, on-line collecting the reaction solution, and then obtaining the saccharose-6-laurate after conventional post-treatment on the reaction solution. The method of the invention has advantages of short reaction time, high selectivity and high yield.

Description

(1) Technical field [0001] The invention relates to a method for lipase-catalyzed on-line controllable selective synthesis of sucrose-6-laurate in a microfluidic channel reactor, which belongs to microfluidic technology and biocatalytic selective synthesis of sucrose esters and other non-ionic surface activities field of agent technology. (2) Background technology [0002] Microfluidics is the technology and science of manipulating nanoliter to picoliter volume fluids in micron-scale structures. It is a new interdisciplinary subject that has risen rapidly in the past decade. At present, the development of microfluidics has greatly surpassed the original purpose of mainly serving analytical chemistry, and is becoming an important technical platform for a new round of innovative research in the entire chemistry discipline, life science, instrument science and even information science. [0003] Since Harrison's research group published the first document on the synthesis of co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12M1/00
Inventor 杜理华罗锡平顾生妹张航诚胡玉婷
Owner ZHEJIANG UNIV OF TECH
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