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Method for preparing brain protein hydrolyzate

A technology of cerebroprotein hydrolyzate and pig brain, which is applied in the field of preparation of cerebroprotein hydrolyzate, can solve the problems of human and environmental hazards, low production efficiency, etc., and achieve the effect of improving stability

Inactive Publication Date: 2013-07-10
黑龙江迪龙制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of low preparation efficiency of the existing method and the use of organic matter in the preparation process, thereby causing harm to people and the environment, and provides a preparation method of cerebroprotein hydrolyzate

Method used

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  • Method for preparing brain protein hydrolyzate
  • Method for preparing brain protein hydrolyzate

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specific Embodiment approach 1

[0009] Specific embodiment one: the preparation method of a kind of cerebroprotein hydrolyzate of this embodiment is carried out through the following steps: 1. Brain tissue homogenate: take pig brain tissue, remove fascia and blood vessel, rinse with purified water, then add 3 times the quality of purified water, homogenate in a homogenizer for 5 to 15 minutes, and use an ultrasonic crusher to ultrasonically treat at an ultrasonic frequency of 25000 Hz for 15 minutes to obtain a homogenate of brain tissue; 2. Degreasing: the homogenate of brain tissue in step 1, Use 10% hydrochloric acid by mass to adjust the pH value to 5.0-5.5, then stand still at 0-10°C for 4-6 hours, then separate with a milk fat separator, remove the turbid liquid, collect the clear liquid, and press the button to collect the clear liquid. 0.5-1.0% of the total weight of the solution, add medicinal diatomite to the clear solution, stir for 30min, then filter with a plate and frame filter, remove the diato...

specific Embodiment approach 2

[0012] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: in step 2, rest at 0-4° C. for 4-6 hours. Others are the same as the first embodiment.

[0013] Prove the beneficial effect of the present invention by following test:

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Abstract

The invention relates to a method for preparing brain protein hydrolyzate. The method is used for solving the problems in existing methods that the production efficiency is low and the injury / damage to human / environment is caused due to the fact that organic matters are used during production. The method comprises the following steps of: (1) carrying out brain tissue homogenization; (2) degreasing; (3) carrying out enzymolysis; (4) carrying out ultrafiltration; and (5) carrying out nanofiltration, thereby obtaining the brain protein hydrolyzate. According to the method, fatty substances are removed from the brain tissue by using purely-mechanical physical methods instead of organic solvent methods, so that the method is very environment-friendly; meanwhile, because no organic matters remain in the brain protein hydrolyzate, the brain protein hydrolyzate is safer; and the fatty substances remaining in the brain protein hydrolyzate are removed by adopting diatomite adsorption twice, so that the stability of drugs is further improved. The method is applied to the field of drug preparation.

Description

technical field [0001] The invention relates to a preparation method of cerebroprotein hydrolyzate. Background technique [0002] Cerebroprotein hydrolyzate is a mixture rich in various amino acids and small molecular polypeptides obtained from animal brain tissue hydrolyzed by protease. Because it contains a large amount of amino acids necessary for human brain and a variety of active peptides for nerve growth, it can regulate And improve the metabolism of neurons. Clinically, cerebroprotein hydrolyzate is mainly used for the recovery of sequelae of craniocerebral injury and cerebrovascular diseases (such as cerebral infarction and cerebral hemorrhage, etc.), and accelerates the functional recovery of the brain nervous system. [0003] Because animal brain tissue contains a large amount of fatty substances, the current preparation method of brain protein hydrolyzate mainly includes two pre-treatment processes, one is to use acetone method to extract the brain tissue homogen...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K35/30
Inventor 董继胜王金富冯文善李永久赵波
Owner 黑龙江迪龙制药有限公司