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A method for supercritical CO2 extraction of gamma-linolenic acid in spirulina

A spirulina and supercritical technology, which is applied in solid solvent extraction, separation/purification of carboxylic acid compounds, bulk chemical production, etc., can solve the problems that have not yet been found to extract γ-linolenic acid patents and literature, and achieve the extraction time Short, low extraction temperature, avoid decomposition and destruction

Active Publication Date: 2015-07-29
YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The same patent and literature on the method of extracting gamma-linolenic acid from spirulina have not yet been found

Method used

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  • A method for supercritical CO2 extraction of gamma-linolenic acid in spirulina
  • A method for supercritical CO2 extraction of gamma-linolenic acid in spirulina

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Embodiment 1

[0034] A supercritical CO 2 Method for extracting gamma-linolenic acid in spirulina, including CO 2 Gas cylinders, high-pressure pumps, temperature controllers, extraction kettles, separation kettles I, separation kettles II, glass balls, filter paper and other equipment, in supercritical CO 2 When extracting gamma-linolenic acid in spirulina, an entrainer is added, which is characterized in that: the entrainer is entrainer A and entrainer B, and salad oil is first used as entrainer A to mix with raw materials and then loaded into the extraction kettle , and then add entrainer B to extract during the extraction process.

[0035] The entrainer B is petroleum ether, and the boiling range of petroleum ether is 90-120°C.

[0036] The addition amount of the entrainer A is 30%-40% of the weight of the raw material powder salad oil; the addition amount of the entrainer B is 80%-150% of the weight of the raw material powder petroleum ether.

[0037]The preferred version of the adde...

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Abstract

The invention discloses a method for extracting gamma-linolenic acid in spirulina through supercritical CO2. The method adopts a CO2 gas bottle, a high-pressure pump, a temperature controller, an extraction kettle, a separation kettle I, a separation kettle II, a glass ball, filter paper and the like; and an entrainer is involved while extracting gamma-linolenic acid in the spirulina through supercritical CO2. The method is characterized in that the entrainer includes an entrainer A and an entrainer B, salad oil, which is taken as the entrainer A, is mixed with raw material and is packed into the extraction kettle together with the raw material, and the entrainer B is added in the extraction process to extract. Obtained extract contains 0.9-1.6% of gamma-linolenic acid in terms of dry weight of spirulina powder, and the extracting rate is greater than 96%.

Description

technical field [0001] The invention belongs to the field of plant extraction, in particular to an extraction method for extracting useful components in spirulina. Background technique [0002] Spirulina (spirulina) is a class of lower plants, belonging to Cyanophyta, Oscillatoriaceae. It is a dark blue filamentous microalgae, which can be observed under a high-power microscope. It is a slender spiral algae composed of single cells and single rows, so it is called Spirulina. Spirulina is rich in protein, polysaccharide, β-carotene, γ-linolenic acid, chlorophyll and various vitamins and minerals. Each gram of spirulina contains 11.8 mg of gamma-linolenic acid, which is 500 times that of breast milk. γ-linolenic acid is a structural material that makes up the biological membranes of various human tissues. Experimental and clinical studies have shown that this product has obvious anti-lipid peroxidation, lowering total cholesterol, increasing high-density lipoprotein, inhibit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02C07C57/12C07C51/47
CPCY02P20/54
Inventor 不公告发明人
Owner YUNNAN BLUE DIAMOND BIOLOGICAL TECH CO LTD