Method for promoting excess sludge microorganisms to hydrolyze by using thermophilic geobacillus sp

A technology of Geobacillus and excess sludge, which is applied in sludge treatment, biological sludge treatment and other directions, can solve the problems of high cost and low microbial biohydrolysis rate of excess sludge, and can improve the cracking rate and the hydrolysis rate. Effect

Inactive Publication Date: 2013-07-10
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the technical problems of low biological hydrolysis rate and high cost of excess sludge microorganisms, thereby providing a method for promoting the hydrolysis of excess sludge microorganisms by Geobacillus thermophiles

Method used

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  • Method for promoting excess sludge microorganisms to hydrolyze by using thermophilic geobacillus sp
  • Method for promoting excess sludge microorganisms to hydrolyze by using thermophilic geobacillus sp

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specific Embodiment approach 1

[0016] Specific embodiment one: a kind of thermophilic geobacillus of the present embodiment promotes the method for microbial hydrolysis of surplus sludge to carry out according to the following steps:

[0017] 1. Inoculate a ring of cryopreserved Geobacillus thermophiles into LB medium, and culture on a rotary shaker at 40-60°C and 100-120r / min for 24-36 hours with ventilation to obtain activated Geobacillus thermophiles;

[0018] 2. Take a ring of activated Geobacillus thermophila and inoculate it in the fermentation medium, and cultivate it at 40-80°C for 12-24 hours to obtain the fermentation broth of Geobacillus thermophila;

[0019] 3. Take 75-200 mL of the supernatant of the Geobacillus thermophila fermented liquid obtained in step 2, and add it to the remaining sludge to obtain a 300-mL mixed solution, and place the mixed solution in a water bath at 60-80°C for 1-6 hours. Complete the thermophilic Geobacillus to promote the microbial hydrolysis of excess sludge;

[0...

specific Embodiment approach 2

[0026] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, culture is carried out on a rotary shaker at 60° C. and 120 r / min for 24 hours with aeration. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0027] Specific embodiment 3: This embodiment differs from specific embodiment 1 or 2 in that: in step 2, culture at 60° C. for 24 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for promoting excess sludge microorganisms to hydrolyze by using thermophilic geobacillus sp, and relates to a method for promoting excess sludge microorganisms to hydrolyze. The invention aims to solve the technical problems of being low in biological hydrolysis rate of excess sludge microorganisms and high in cost. The method disclosed by the invention comprises the following steps of: 1, obtaining activated thermophilic geobacillus sp; 2, inoculating activated thermophilic geobacillus sp into a fermented culture medium to culture, and obtaining fermentation liquid of thermophilic geobacillus sp; and 3, adding supernate of fermentation liquid of thermophilic geobacillus sp into excess sludge to react, so that hydrolysis promotion of excess sludge microorganisms by using thermophilic geobacillus sp is completed. The method disclosed by the invention is capable of promoting the cracking rate of microorganisms and increasing the hydrolysis rate of sludge and is applicable in the technical field of hydrolysis of microorganisms.

Description

technical field [0001] The invention relates to a method for promoting microbial hydrolysis of excess sludge. Background technique [0002] The wastewater treatment process is accompanied by the generation of a large amount of solid waste, the most important component of which is excess sludge. In China, in 2008, the output of excess sludge (80% moisture content) was as high as 18 million tons per year, and it continued to grow at a rate of 10% per year. Its treatment and disposal cost accounts for about 60% of the total operating cost of the sewage treatment plant and more than 40% of the total investment cost. The proportion of microorganisms in the remaining sludge reaches more than 70%, and is dominated by Gram-negative bacteria, and is rich in various organic carbon sources, such as: protein, carbohydrates and lipids. At present, for the treatment and disposal of excess sludge, more and more researches focus on the utilization of excess sludge as a cheap and valuable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02
CPCY02W10/20
Inventor 王爱杰杨春雪周爱娟郭泽冲侯雅男
Owner HARBIN INST OF TECH
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