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Stirring-type and air-lifting combined bioreactor and application thereof in preparing rhamnose gum

A technology of stirred reactor and airlift reactor, which is applied in the application field of rhamnose gum preparation, can solve the problems of unseen fermentation research and industrial production reports, and achieve the improvement of mass transfer rate and dissolved oxygen level , small shear force, and the effect of reducing the apparent viscosity

Inactive Publication Date: 2013-07-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on rhamnose gum in China is still blank, and there are no relevant fermentation research and industrial production reports.

Method used

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  • Stirring-type and air-lifting combined bioreactor and application thereof in preparing rhamnose gum
  • Stirring-type and air-lifting combined bioreactor and application thereof in preparing rhamnose gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: A 3L stirred reactor used glucose as a carbon source to produce rhamnose gum in a single batch of free cell fermentation (no airlift reactor).

[0032] Using glucose as carbon source, rhamnan gum was produced by single-batch fermentation of free cells of Sphingomonas sp.RH-1 (CGMCC NO.6833), which has been patented by our laboratory.

[0033] Seed medium: glucose 10g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O 0.5g / L, use NaOH solution to adjust the pH to 6.8.

[0034] Fermentation medium: glucose 40g / L, yeast extract 5g / L, (NH 4 ) 2 SO 4 5g / L, K 2 HPO 4 ·3H 2 O 2g / L, MgSO 4 ·7H 2 O 0.5g / L, use NaOH solution to adjust the pH to 6.8.

[0035] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the condition of 30°C and 200r / min shaker for 18h, and the seed solution is 8% (v / v) Inoculate in a 3L stirred reactor 11 pre-installed with 2.1L sterilized fermentation medium fo...

Embodiment 2

[0036] Example 2: A 3L stirred and air-lift bioreactor used glucose as a carbon source for single-batch fermentation to produce rhamnose gum.

[0037] Bacterial strain, seed culture medium and fermentation medium are the same as embodiment 1.

[0038] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the condition of 30°C and 200r / min shaker for 18h, and the seed solution is 8% (v / v) Inoculate in a 3L stirred reactor 11 pre-installed with 2.6L sterilized fermentation medium for cultivation, turn on the constant flow pump 2-3 to realize the material exchange in the stirred reactor 11 and the airlift reactor 12, Turn on the constant temperature water bath 13 to realize that the temperature of the material in the airlift reactor 12 is constant at 30°C. The reaction conditions of the stirred reactor are: 30°C, 600r / min, and the ventilation rate is 2.3L / min. During the fermentation process, the pH automatic ...

Embodiment 3

[0039] Example 3: Multi-batch fermentation of rhamnose gum by using glucose as a carbon source in a 3L stirred and air-lift bioreactor.

[0040] Bacterial strain, seed culture medium and fermentation medium are the same as embodiment 1.

[0041] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the conditions of 30°C and 200r / min shaker for 18h, and the seed solution is 10% (v / v) of the seed amount Inoculate in a 3L stirred reactor 11 pre-installed with 2.6L sterilized fermentation medium for cultivation, turn on the constant flow pump 2-3 to realize the material exchange in the stirred reactor 11 and the airlift reactor 12, Turn on the constant temperature water bath 13 to realize that the temperature of the material in the airlift reactor 12 is constant at 32°C. The reaction conditions of the stirred reactor are: 32°C, 1000r / min, and the ventilation rate is 2.3L / min. During the fermentation process, t...

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Abstract

The invention discloses a stirring-type and air-lifting combined bioreactor which consists of a stirring-type reactor part and an air-lifting reactor part, which are connected with each other through a constant-current pump so as to exchange materials. The stirring-type reactor part consists of a stirring-type reactor, an automatic pH control device, a replenishing device, a first ventilation device and a stirring device; and the air-lifting reactor part consists of an air-lifting reactor, a constant-temperature water bath and a second ventilation device. The invention also discloses an application of the stirring-type and air-lifting combined bioreactor in fermenting and preparing the rhamnose gum. By utilizing the device and the process, the production intensity of the rhamnose gum is high, the product concentration is high, industrialization can be realized, and an efficient production method can be realized.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and process engineering, and relates to a bioreactor combined with stirring type and airlift type and its application in the preparation of rhamnose gum. Background technique [0002] Rhamnan Gum (Rhamsan Gum) is an exopolysaccharide produced by Sphingomonas, a kind of gellan gum polysaccharide, also known as S-194. Gellan gum polysaccharides have the same main chain structure of four sugar repeating units: D-glucose, D-glucuronic acid, D-glucose and L-rhamnose, but there are some slight differences in side chains or other structures. The structure of rhamnose gum is shown in (1). With its unique physical and rheological properties and safety in use, rhamnose gum can be widely used as suspending agent, stabilizer, thickener, emulsifier, film-forming agent, etc. in food, petroleum, cement, coatings, etc. industry. [0003] [0004] At present, the research on rhamnose gum in...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/02C12M1/06C12P19/04C12R1/01
Inventor 徐虹徐晓滢朱萍李莎梁金丰陈晓晔
Owner NANJING UNIV OF TECH
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