Method for propagating citrus and pomelo seedlings resistant to citrus greening disease by chemical mutagenesis

Abstract

The invention relates to a method for propagating citrus and pomelo seedlings resistant to citrus greening disease by chemical mutagenesis and belongs to the technical field of seedling propagation. The method is achieved by chemical mutagenesis and includes: selecting a citrus / pomelobudstick suffering from citrus greening disease as a scion, treating with sodium nitrite drug for the first time, treating with disodium hydrogen phosphate drug for the second time, flushing with fresh water, grafting for the first time to form a mutant, grafting for the second time to form a disease resistant, grafting for the third time to form a disease-resisting seedling, testing exterior syndromes, and propagating and popularizing. The disease-resisting seedling propagated by the method is resistant. After planted in a field and even infected with disease-carrying Diaphorinacitri, the seedling which is resistant and immune is capable of killing citrus greening disease invaded inside, and immunizing and disease resisting are achieved. The method is effective in restraining the citrus greening disease from spreading and conducive to promoting great development of citrus and pomelo industries in Guangdong province.

Description

[0001] technical field [0002] The invention relates to a seedling breeding method, in particular to a method for breeding citrus pomelo seedlings resistant to Huanglongbing by chemical mutagenesis, and belongs to the technical field of seedling breeding. Background technique [0003] Citrus pomelo huanglongbing (Liberobacter asiaticum) is considered to be a devastating disease of citrus pomelo production in the world. Infected citrus pomelo varieties lose fruiting ability within 3 to 5 years after getting sick, and even die. In my country, the disease is currently distributed in Guangdong, Guangxi, Hainan, Fujian, Hunan, Guizhou, Yunnan, Sichuan, Chongqing, Jiangxi, Zhejiang, and Taiwan provinces, cities, and districts south of the Yangtze River; abroad, it is mainly distributed in Southeast Asia in Asia. , Indian Peninsula, Arabian Peninsula; in addition, some countries in Africa, Brazil and parts of the United States also have. [0004] In the 1970s, the citrus field...

AI Technical Summary

Problems solved by technology

The prevention and control of the three major measures is a series of technical system engineering, which requires the combination of government actions and technical guidance. If the fruit seedling quarantine system is not strictly implemented, the diseased seedlings will flow into the market and the Huanglong disease trees in the orchards of thousands of households will not be cut down. Drugs to kill citrus psyllids are not uniformly deployed, leaving many future troubles, which will inevitably lead to failure to prevent and control

[0009] ④ Huanglongbing bacteria are seriously harmful in the phloem vascular bundles of citrus pomelo plants, causing obstacles to the transportation of organic nutrients in the plants and causing yellowing

[0011] The inventor has always regarded the prevention and treatment of citrus and pomelo huanglongbing as a key research topic, and has done a lot of research work, including: breeding of disease-free seedlings; application of various antibiotics for high-pressure injection control research; improvement of fertilization technology; use of naphthalene acetic acid rooting agent , ammonia-resistant hydrogen-fixing bacteria, "802" regulator and other root-promoting experiments; research on the prevention and treatment of citrus psyllids; and experimental research on high-level diseased tree grafting and improvement of yellowed trees, all of which have not received results