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Black mushroom extractive and application thereof

The technology of extract and black oyster mushroom is applied in the field of black oyster mushroom extract, which can solve the problems of enhancing immunity of polysaccharide of black oyster mushroom strain, and achieve the effects of being suitable for production and application, simple extraction process and improved thymus coefficient.

Active Publication Date: 2014-12-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report about the enhancement of immunity by polysaccharides of black oyster mushroom strains at home and abroad

Method used

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  • Black mushroom extractive and application thereof
  • Black mushroom extractive and application thereof
  • Black mushroom extractive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of black oyster mushroom extract of the present invention

[0028] The preparation of black oyster mushroom extract is carried out according to the following method:

[0029] 1) Dry the black oyster mushroom at 30-40°C and crush it into powder, pass through a 60-200 mesh sieve to obtain 2g of black oyster mushroom dry powder and mix it with 200ml of ethanol solution with a concentration of 85% by volume, and dry it at 60°C Reflux extraction in a water bath for 45 minutes to remove small molecular substances such as monosaccharides and disaccharides, filter, and collect the filter residue;

[0030] 2) Add distilled water to the above filter residue, the solid-liquid ratio is 1g:30ml, reflux extraction in a 95°C water bath for 1 hour, filter, and collect the filtrate and filter residue respectively; take the filter residue and repeat this step three times, and combine all the filtrates.

[0031] 3) Add trichloroacetic acid (TCA) to the filtr...

Embodiment 2

[0035] Embodiment 2, the preparation of black oyster mushroom extract of the present invention

[0036] The preparation of black oyster mushroom extract is carried out according to the following method:

[0037] 1) Strain black oyster mushroom dry powder (preparation of black oyster mushroom dry powder is the same as in Example 1) 2g is mixed with 600ml of ethanol solution with a concentration of 70% by volume, and refluxed in a water bath at 50°C for 60 minutes to remove monosaccharides and double polysaccharides Sugar and other small molecular substances, filter and collect the filter residue;

[0038] 2) Add distilled water to the above filter residue, the solid-liquid ratio is 1g: 100ml, reflux extraction in a 70°C water bath for 2 hours, filter, collect the filtrate and filter residue respectively; take the filter residue and repeat this step 4 times, and combine all the filtrates.

[0039] 3) Add trichloroacetic acid (TCA) to the filtrate obtained in step 2) (to make th...

Embodiment 3

[0043] Embodiment 3, the preparation of black oyster mushroom extract of the present invention

[0044] The preparation of black oyster mushroom extract is carried out according to the following method:

[0045] 1) Strain black oyster mushroom dry powder (preparation of black oyster mushroom dry powder is the same as in Example 1) 2g is mixed with 200ml of ethanol solution with a concentration of 90% by volume, and reflux extraction in a water bath at 70°C for 30 minutes to remove monosaccharides and double polysaccharides Sugar and other small molecular substances, filter and collect the filter residue;

[0046] 2) Add distilled water to the above filter residue, the solid-liquid ratio is 1g: 10ml, reflux extraction in a water bath at 100°C for 1 hour, filter, and collect the filtrate and filter residue respectively; take the filter residue and repeat this step once, and combine all the filtrates.

[0047] 3) Add trichloroacetic acid (TCA) to the filtrate obtained in step 2) (...

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Abstract

The invention discloses a black mushroom extractive and application thereof. A preparation method of the black mushroom provided by the invention comprises the following steps: 1) mixing the dry powder of black mushroom and an alcohol solution with volume percent concentration of 70-90%, heating to remove micromolecular matters such as monosaccharide and glycan, filtering and collecting filter residues; 2) mixing the filter residue with distilled water in proportion of 1g: 10-100ml, extracting for 1-2 hours by water bath with temperature of 70-100 DEG.C, filtering, respectively collecting filtrate and filter residues, repeating the operation step for 1-4 times and mixing all the filtrate; 3) adding trichloroacetic acid in the filtrate, removing protein out of the filtrate, centrifuging, and collecting the supernate; 4) adding hydrogen peroxide in the supernate, discoloring, concentrating to obtain the solution containing the black mushroom extractive. The black mushroom extractive disclosed by the invention can be used for preparing the medicine or food for enhancing immunity, the preparation method is simple, the raw materials are easily available, the cost is low, and the black mushroom extractive is suitable for production and application.

Description

technical field [0001] The invention relates to a black oyster mushroom extract and its application. Background technique [0002] Improving immunity is an important part of the healthy life advocated by the Ministry of Health of my country. It is of great significance to enhance the body's resistance to disease, anti-infection, anti-tumor and maintain its own physiological balance. The effective way to prevent and improve immunocompromised diseases and immunodeficiency is to improve the dietary structure in nutrition, but most of the immune enhancers used in clinical practice come from chemically synthesized drugs, most of which have toxic side effects Or the problem of high cost, there are certain limitations in application. However, natural food extracts often have the advantages of low toxicity and low side effects. Therefore, screening natural active ingredients with high safety to human body and low cost is an effective way to solve this problem. Studies have shown t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61P37/04A23L1/28A23L1/29A23L33/00
Inventor 燕继晔李兴红刘建华严红赵爽周莹张玮乔广行
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES