High-resolution tomography optical microscopic imaging device for tissue samples

Abstract

The invention discloses a high-resolution tomography optical microscopic imaging device for tissue samples. The high-resolution tomography optical microscopic imaging device based on mechanical cutting can cut the tissue samples into thin slices and simultaneously carry out microscopic optical imaging for the thin slices. The device comprises a tissue sample cutting module and an optical microscopic imaging system. A cutting tool of the device is subjected to opacity treatment. The high-resolution tomography optical microscopic imaging device has the advantages that the slices are physically separated from remaining tissue samples in a cutting procedure while tomography images are acquired, illumination light or excitation light of the optical microscopic system cannot be transmitted through the thin slices and the cutting tool to illuminate or excite the remaining tissue samples below the cutting tool owing to the opacity treatment for the cutting tool, and accordingly interference of the tissue samples which are not cut currently on sampling for the slices is eliminated. The high-resolution tomography optical microscopic imaging device can be used for quick high-resolution tomography microscopic imaging for tissue samples.

Description

technical field [0001] The invention is a device for realizing tomographic microscopic imaging of tissue samples based on mechanical cutting. Background technique [0002] High-resolution tomographic optical microscopy imaging of tissue samples, followed by three-dimensional reconstruction, can obtain the three-dimensional spatial distribution of subcellular structures. For neural tissues, the three-dimensional spatial distribution of dendrites and axons of specific neurons can be tracked. To understand the interconnection relationship between neurons. [0003] In order to realize tomographic optical microscopic imaging of tissue samples, optical tomographic imaging technology can be used: confocal imaging technology, through the use of confocal pinholes to block signals outside the focal plane, to achieve tomography; two-photon microscopic imaging technology, using non- Linear effect, the effective optical signal only comes from the focus position to achieve tomography; in...

AI Technical Summary

Problems solved by technology

The thin sections of tissue samples cut in this way are spatially separated from the rest of the tissue samples (depending on the thickness of the knife), but since thin sections of plastically embedded tissue samples are usually made of transparent diamond knives, the The remaining sample under the diamond knife will still affect the optical imaging results through the diamond knife, and the interference background will be superimposed on the image

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