32results about How to "No background distractions" patented technology

The invention belongs to the field of mass-spectral imaging, and in particular relates to an image acquisition semiconductor film for a high-resolution mass-spectral imaging system, and a preparation method and application of the image acquisition semiconductor film. The preparation method of the image acquisition semiconductor film for the high-resolution mass-spectral imaging system comprises the following steps: weighing semiconductor nano-particles, putting the semiconductor nano-particles into a muffle furnace for calcining firstly, further grinding by using an agate mortar, and uniformly dispersing the semiconductor nano-particles so as to obtain semiconductor nano-powder; and finally, pressing the semiconductor nano-powder in a tablet press so as to obtain the semiconductor film. By utilizing the laser-induced tunnel electron capture principle of a semiconductor nano-material, a sample is molecularized and ionized without background interference; the limitation of a conventional MALDI substrate is overcome; the semiconductor film is simple and easy to obtain, steady in mass-spectral signal, uniform and smooth in surface and free of the background interference, and can be used for fingerprint analysis and animal and plant tissue slice analysis; the semiconductor film is particularly applied to accurate mass-spectral imaging of small molecular substances; and quality control and industrialization are carried out conveniently.

The invention provides a method for preparing a paraffin section of an oocyte. The method of the invention comprises the steps of fixing, dehydrating, transparency processing, paraffin embedding and slicing, etc., the method of the invention leads the oocyte to be transferred in the circumstance of liquid during the processes of fixing, dehydrating, transparency processing, etc. without a direct effect from outer force, thus the original morphosis of the oocyte can be maintained in maximum extent; a colorless transparent vessel is used for being filled with the oocyte, to lead the oocyte to beseen clearly; after the oocyte is transferred to a mould box, a xylene is removed to reveal the oocyte, thus the oocyte can be found more easily; before the paraffin embedding, a plurality of coloredgranules are scattered around the oocyte, thereby being convenient for the location of the oocyte in the paraffin. The method of the invention can make a paraffin section of a few or even a single oocyte of cursorial bird with the diameter of 100 microns to 600 microns, the structure of the gained section is clear and complete, and the staining effect is good.

The invention belongs to the field of mass-spectral imaging, and in particular relates to an image acquisition semiconductor film for a high-resolution mass-spectral imaging system, and a preparation method and application of the image acquisition semiconductor film. The preparation method of the image acquisition semiconductor film for the high-resolution mass-spectral imaging system comprises the following steps: weighing semiconductor nano-particles, putting the semiconductor nano-particles into a muffle furnace for calcining firstly, further grinding by using an agate mortar, and uniformly dispersing the semiconductor nano-particles so as to obtain semiconductor nano-powder; and finally, pressing the semiconductor nano-powder in a tablet press so as to obtain the semiconductor film. By utilizing the laser-induced tunnel electron capture principle of a semiconductor nano-material, a sample is molecularized and ionized without background interference; the limitation of a conventional MALDI substrate is overcome; the semiconductor film is simple and easy to obtain, steady in mass-spectral signal, uniform and smooth in surface and free of the background interference, and can be used for fingerprint analysis and animal and plant tissue slice analysis; the semiconductor film is particularly applied to accurate mass-spectral imaging of small molecular substances; and quality control and industrialization are carried out conveniently.

The invention discloses a preparation method of a functionalized rare earth long-lasting nanocomposite material and its application in latent fingerprint imaging, belonging to the technical field of biological analysis and fingerprint detection. Avidin was modified to the surface of amino-functionalized strontium aluminate rare earth doped long-lasting nanomaterials to prepare avidin-functionalized rare-earth long-lasting nanocomposites, and then using the recognition function between biotin and avidin, the Avidinylated rare earth long-persistence nanocomposites attached to latent fingerprints labeled with biotinylated antibodies. The strontium aluminate rare earth doped long afterglow nanomaterial has long afterglow characteristics, and after being irradiated by a light source and removed, it still keeps emitting light, which can realize latent fingerprint imaging. The method of the invention is fast, simple and has no background interference.

The invention relates to anti-liver cancer derived growth factor monoclonal antibody and an application of the same. The invention makes the specific monoclonal antibody of anti-hepatoma derived growth factor (HDGF), wherein the monoclonal antibody can be used to make HDGF detection reagent suitable for a plurality of detection method.

The invention provides a human palm print image acquiring device and a processing method. The human palm print image acquiring device consists of a synchronization unit, a sensor unit, a control unit and a computer. The synchronization unit is connected with the sensor unit. The sensor unit is connected with the control unit. The control unit is connected with the computer. The synchronization unit consists of a multiple-reflection optical system, a roller pair, a gear bank and a photoelectric encoder. The sensor unit comprises a CCD (Charge Coupled Device) linear array image sensor and a CIS (Contact Image Sensor) linear array image sensor module. The control unit is a linear array palm print image acquisition control unit on the basis of an FPGA (Field Programmable Gate Array) and a USB (Universal Serial Bus). According to the invention, under the condition of keeping the image quality and the identification precision, the volume is reduced. A palm print image acquired by the human palm print image acquiring device can be spliced into various resolutions such as 100dpi, 200dpi and the like to adapt to different requirements. According to the invention, the mobile palm print identification can be realized and meanwhile, the human palm print image acquiring device can also be used as a fixed palm print identification device. Two different functions can be achieved by one piece of equipment. The human palm print image acquiring device is flexible and is easy to use.

The invention belongs to the technical field of detection in food, and in particular relates to the preparation of an electrolytic solution and its use for electrochemical rapid detection of the content of xanthanin; the method is as follows: firstly, an electrolytic solution is provided, which is composed of a polar solvent and a supporting electrolyte ; Then extract the peppercornin mother liquor of the sample, dilute it with electrolyte to obtain the solution to be tested, add α-SOH standard solution, and obtain a mixed solution; reconfigure the solution to be tested with equal concentration and volume, and add 2 times the volume of α-SOH standard solution , to obtain the mixed solution; repeating this many times, finally using the three-electrode system to detect the differential pulse voltammetry curve of the pepperonamide substance in the mixed solution respectively; There is no background interference during detection; in the specific detection process, it has the advantages of fast detection, sensitivity, simplicity, high measurement accuracy, short sample analysis time, low cost, and convenient online detection.

Glass thin slice with the multiple reaction ponds is set up on the outer casing of the compound chip for detecting oneself immunity antibody of diabetes. the detecting micro array is built in each pond. Four different kinds of protein solid phase detecting pieces in mini type with sample specification size constitute each detecting micro array. These detecting pieces are the piece of the islet cells, the piece of GAD protein, the PTP protein and the Insulin protein. First, these detecting pieces are prepared, then these pieces are assembled. The detecting method includes two steps: first, the indirect fluorescence method is used; then, the biochip laser co-focusing scanner determines the result. Comparing with the prior art the invention possesses the advantages of the accurate clinical analysis and the low cost.

The invention relates to a method for detecting residual organics on a fire scene based on a surface enhancing raman spectrum. The method comprises the following steps: constructing a detection substrate material which comprises a metal film and a nanometer particle, wherein the surface of the metal film is modified with functional groups, the metal film has a hydrophobic effect and is smooth and flat, and the nanometer particle has surface enhancing raman activity and is free from modification with the functional groups; dropwise adding the filtration liquid onto the surface of the metal film with the hydrophobic effect; after finishing the dropwise adding, naturally volatilizing the liquid; dropwise adding the colloid containing the nanometer particle with the surface enhancing activity on the surface of the metal film and utilizing a raman spectrometer to detect the colloid on the surface of the metal film; comparing and analyzing the acquired spectrum chart and identifying the category of the organic compound in the to-be-detected article. According to the method, the operation is simple and convenient; the detecting base structure is simple and can be directly used for extracting, absorbing and enriching the analyte in the solution sample; the designed and constructed enhancing system can realizably acquire high-quality SERS test result; the method has the advantages of high sensitivity, excellent repeatability, zero background interference, and the like.

The invention discloses a vehicle speed matching method based on linear array CCD image, including the following steps that: two linear array CCD video cameras are respectively arranged on two sections positioned on a road at intervals; both linear array CCD video cameras face downwards and are provided with infrared ray laser source; the frame rate of each CCD video camera is more than 1,000 frames per second, and the resolution is more than 1,024 lines; correlation matching computation of the image sequences obtained by both linear array CCD video cameras is carried out to obtain the time difference of the same object in two image sequences; finally, by means of the time difference and the distance between both cameras, the driving speed of an object vehicle can be obtained according tothe velocity formula V=s / t. The invention is suitable for vehicle speed measurement at a traffic station, etc.