32results about How to "No background distractions" patented technology

The invention provides a method for preparing a paraffin section of an oocyte. The method of the invention comprises the steps of fixing, dehydrating, transparency processing, paraffin embedding and slicing, etc., the method of the invention leads the oocyte to be transferred in the circumstance of liquid during the processes of fixing, dehydrating, transparency processing, etc. without a direct effect from outer force, thus the original morphosis of the oocyte can be maintained in maximum extent; a colorless transparent vessel is used for being filled with the oocyte, to lead the oocyte to be seen clearly; after the oocyte is transferred to a mould box, a xylene is removed to reveal the oocyte, thus the oocyte can be found more easily; before the paraffin embedding, a plurality of colored granules are scattered around the oocyte, thereby being convenient for the location of the oocyte in the paraffin. The method of the invention can make a paraffin section of a few or even a single oocyte of cursorial bird with the diameter of 100 microns to 600 microns, the structure of the gained section is clear and complete, and the staining effect is good.

Glass thin slice with the multiple reaction ponds is set up on the outer casing of the compound chip for detecting oneself immunity antibody of diabetes. the detecting micro array is built in each pond. Four different kinds of protein solid phase detecting pieces in mini type with sample specification size constitute each detecting micro array. These detecting pieces are the piece of the islet cells, the piece of GAD protein, the PTP protein and the Insulin protein. First, these detecting pieces are prepared, then these pieces are assembled. The detecting method includes two steps: first, the indirect fluorescence method is used; then, the biochip laser co-focusing scanner determines the result. Comparing with the prior art the invention possesses the advantages of the accurate clinical analysis and the low cost.

The invention provides a human palm print image acquiring device and a processing method. The human palm print image acquiring device consists of a synchronization unit, a sensor unit, a control unit and a computer. The synchronization unit is connected with the sensor unit. The sensor unit is connected with the control unit. The control unit is connected with the computer. The synchronization unit consists of a multiple-reflection optical system, a roller pair, a gear bank and a photoelectric encoder. The sensor unit comprises a CCD (Charge Coupled Device) linear array image sensor and a CIS (Contact Image Sensor) linear array image sensor module. The control unit is a linear array palm print image acquisition control unit on the basis of an FPGA (Field Programmable Gate Array) and a USB (Universal Serial Bus). According to the invention, under the condition of keeping the image quality and the identification precision, the volume is reduced. A palm print image acquired by the human palm print image acquiring device can be spliced into various resolutions such as 100dpi, 200dpi and the like to adapt to different requirements. According to the invention, the mobile palm print identification can be realized and meanwhile, the human palm print image acquiring device can also be used as a fixed palm print identification device. Two different functions can be achieved by one piece of equipment. The human palm print image acquiring device is flexible and is easy to use.

The invention belongs to immunity detection area, especially related to method for quantitative measuring proteins and peptides with molecular weight under 20kDa. Gold-coated slide glass or triangular prism is as detection substrate. The method includes following procedures: forming an assembling body, forming duality immune complex of first antibody - antigen, forming sandwich ternary immune complex of first antibody - antigen - second antibody as well as resonant wavelength testing. The antigens to be detected are insulin bFGF, T3 or T4 etc. the first antibody and second antibody are first and second monoclone antibodies possessing specificities and complementarity for anti each antigen to be detected. The invention monitors dynamic reaction procedure of biomoleculars, and tests change of concentration of object to be detected with high sensitivity. The combination of second anti body generates stronger optical signal, creating biologic amplified activate.

The invention relates to a human alpha-defensin peptide enzyme linked immunosorbent assay kit and a preparation and detection method thereof. The human alpha-defensin peptide enzyme linked immunosorbent assay kit comprises a mouse-anti-human HNP monoclonal antibody coated ELISA plate, an HNP protein freeze-dried standard substance, a rabbit anti-human HNP polyclonal antibody, a horseradish peroxidase labeled goat anti-rabbit immune globulin polyclonal antibody, standard diluent, sample diluent, a 25-fold concentrated washing buffer solution, antibody diluent, a TMB developing solution and an enzyme reaction terminating solution. The preparation method comprises the following steps: coupling alpha-defensin and a carrier protein, adopting a coupling compound with the optimal coupling binding ratio (the molar ratio of HNP to SPDP to mouse serum albumin is 1:5:1, and the molar ratio of HNP to SPDP to rabbit serum albumin is 1:5:1, preparing to obtain a high-sensitivity antibody by using a complete antigen, and assembling the kit by using the obtained antibody. The kit disclosed by the invention has the advantages of high detection sensitivity, accurate result, wide detection range, simplicity and convenience in operation, long preservation time and the like.

The invention, which belongs to the technical field of detection in food, particularly relates to preparation of an electrolytic solution and the use thereof for electrochemically detecting the content of sanshool quickly. The method comprises: providing an electrolytic solution containing a polar solvent and a supporting electrolyte; extracting a sanshool mother solution of a sample, carrying outdilution by using the electrolytic solution to obtain a to-be-tested solution, and adding an alpha-SOH standard solution to obtain a mixed solution; preparing an equal-concentration equal-volume to-be-tested solution again and adding two-times-volume alpha-SOH standard solution to obtain a mixed solution; repeating the process by multiple times and then detecting differential pulse voltammetry curves of sanshool substances in the mixed solution respectively by using a three-electrode system; and calculating the content of sanshool by combining a formula. The electrolytic solution has conductivity and no background interference exists during detection. The electrolytic solution has advantages of fast detection, high sensitivity, simpleness, high measurement accuracy, short time required for sample analysis, low cost, and great convenience in on-line detection and the like in the specific detection process.

The invention provides a micro-plasma excitation source based on ultrasonic atomization sample introduction, and relates to the field of emission spectrum element analysis excitation light sources. The micro-plasma excitation source comprises an AC power supply, two metal electrodes and an ultrasonic atomization sheet; the AC power supply is electrically connected with one end of each metal electrode; the discharge ends of the metal electrodes are positioned in the same plane; the ultrasonic atomization sheet is horizontally arranged above the discharge ends of the metal electrodes and is used for converting a solution to be detected into aerosol and downwards spraying the aerosol to the discharge ends of the metal electrodes and space between the metal electrodes; and the AC power supply, the metal electrodes and the aerosol are matched to form a V-shaped micro plasma below the metal electrodes. The invention further provides an excitation method adopting the micro-plasma excitation source based on ultrasonic atomization sample introduction, and the detection precision of metal elements can be effectively improved.

The invention belongs to the technical field of immunological detection, and particularly relates to preparation of a near-infrared light-emitting Yb and Tm co-doped core-shell structure nanocrystal and application of the nanocrystal in immunochromatographic detection. A synthesis scheme of the core-shell structure nanocrystal is adopted, by means of the scheme, sensitizing ions Yb can be highly doped to 98%, and therefore, the absorption capacity of the nanocrystal to 980nm light is improved; meanwhile, the doping proportion of luminous ions Tm ions in the middle shell layer is determined to be 2%. Under the proportion, a cross relaxation quenching phenomenon and the balance of effective energy transfer between Yb and Tm are realized, and finally, the near-infrared luminous intensity of the core-shell structure nanocrystal is greatly improved (the luminous intensity is improved by 850 times compared with that of standard Yb and Tm bare core nanocrystals). An immunochromatography detection method is established by using the high-brightness near-infrared light-emitting nanocrystal as an immunofluorescence probe, thereby realizing the immunochromatography method for detecting disease indexes without background interference and with high sensitivity.