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32results about How to "No background distractions" patented technology
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Method for preparing oocyte paraffin cut film
InactiveCN101144760AReduce morphological and structural changesLow costPreparing sample for investigationBiological testingParaffin waxChemistry
The invention provides a method for preparing a paraffin section of an oocyte. The method of the invention comprises the steps of fixing, dehydrating, transparency processing, paraffin embedding and slicing, etc., the method of the invention leads the oocyte to be transferred in the circumstance of liquid during the processes of fixing, dehydrating, transparency processing, etc. without a direct effect from outer force, thus the original morphosis of the oocyte can be maintained in maximum extent; a colorless transparent vessel is used for being filled with the oocyte, to lead the oocyte to be seen clearly; after the oocyte is transferred to a mould box, a xylene is removed to reveal the oocyte, thus the oocyte can be found more easily; before the paraffin embedding, a plurality of colored granules are scattered around the oocyte, thereby being convenient for the location of the oocyte in the paraffin. The method of the invention can make a paraffin section of a few or even a single oocyte of cursorial bird with the diameter of 100 microns to 600 microns, the structure of the gained section is clear and complete, and the staining effect is good.
Owner:CHINA AGRI UNIV
Method for matching vehicle speed based on linear array CCD image
InactiveCN101206229AThe speed measurement method works wellHigh resolutionRoad vehicles traffic controlDevices using time traversedImage resolutionTime difference
The invention discloses a vehicle speed matching method based on linear array CCD image, including the following steps that: two linear array CCD video cameras are respectively arranged on two sections positioned on a road at intervals; both linear array CCD video cameras face downwards and are provided with infrared ray laser source; the frame rate of each CCD video camera is more than 1,000 frames per second, and the resolution is more than 1,024 lines; correlation matching computation of the image sequences obtained by both linear array CCD video cameras is carried out to obtain the time difference of the same object in two image sequences; finally, by means of the time difference and the distance between both cameras, the driving speed of an object vehicle can be obtained according to the velocity formula V=s / t. The invention is suitable for vehicle speed measurement at a traffic station, etc.
Owner:CHANGAN UNIV +2
ESPR-based heavy metal analyzer and analysis method thereof
InactiveCN102628798ADetection interference is smallResponsivePhase-affecting property measurementsScattering properties measurementsSurface plasmon resonance spectroscopyInformation processing
The invention discloses an ESPR-based heavy metal analyzer, which comprises a chemical reaction part, a surface plasma resonance spectrometer and an information processing system, wherein the chemical reaction part includes a detection cell, a counter electrode, a reference electrode, an array electrode and an electrochemical workstation; the array electrode is positioned at the bottom of the detection cell, the counter electrode and the reference electrode are inserted in the detection cell, the counter electrode, the reference electrode and the array electrode are respectively connected to the electrochemical workstation; the electrochemical workstation and the surface plasma resonance spectrometer are respectively connected to the information processing system. The invention further discloses an analysis method of the ESPR-based heavy metal analyzer, which achieves the simultaneous detection of multi-objective heavy metals, enhances the detection sensitivity and spatial resolution, and reduces damages on thin metallic membrane plate (the array electrode and active points thereof).
Owner:GUANGZHOU INGSENS SENSOR TECH
Image acquisition semiconductor film for high-resolution mass-spectral imaging system, and preparation method and application of image acquisition semiconductor film
ActiveCN104597113AOvercome limitationsNo background distractionsMaterial nanotechnologyZinc oxides/hydroxidesPhysical chemistryMass spectrometry imaging
The invention belongs to the field of mass-spectral imaging, and in particular relates to an image acquisition semiconductor film for a high-resolution mass-spectral imaging system, and a preparation method and application of the image acquisition semiconductor film. The preparation method of the image acquisition semiconductor film for the high-resolution mass-spectral imaging system comprises the following steps: weighing semiconductor nano-particles, putting the semiconductor nano-particles into a muffle furnace for calcining firstly, further grinding by using an agate mortar, and uniformly dispersing the semiconductor nano-particles so as to obtain semiconductor nano-powder; and finally, pressing the semiconductor nano-powder in a tablet press so as to obtain the semiconductor film. By utilizing the laser-induced tunnel electron capture principle of a semiconductor nano-material, a sample is molecularized and ionized without background interference; the limitation of a conventional MALDI substrate is overcome; the semiconductor film is simple and easy to obtain, steady in mass-spectral signal, uniform and smooth in surface and free of the background interference, and can be used for fingerprint analysis and animal and plant tissue slice analysis; the semiconductor film is particularly applied to accurate mass-spectral imaging of small molecular substances; and quality control and industrialization are carried out conveniently.
Owner:HUAZHONG NORMAL UNIV
A traffic information acquisition system based on linear array CCD video camera imaging
InactiveCN101216997AThe speed measurement method works wellHigh resolutionDetection of traffic movementClosed circuit television systemsSplit linesCcd camera
The invention discloses a traffic information acquiring system based on linear CCD imaging, which comprises two linear CCD cameras spaced at a certain interval and disposed on a support above two road sections, each is provided with an auxiliary lighting headlight; and a plurality of auxiliary planar array cameras arranged at the position 15 m away from the front of the two linear CCD cameras and respectively located on the middle of a driveway and on the separating line of the driveway, wherein the auxiliary planar array cameras in the middle of the driveway can shoot vehicles passing the road, and the auxiliary planar array cameras in the driveway separating line can perform panning shot. When the vehicles pass the road section, the linear CCD cameras can acquire linear image data of the vehicles passing the road section; the acquired linear image data is processed and analyzed to obtain traffic parameters such as traffic flow, vehicle velocity, traffic stream density and road occupation rate, and the planar array cameras can shoot vehicle image which is analyzed and processed to obtain license plate information.
Owner:CHANGAN UNIV
Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof
InactiveCN1423131AReduce testing costsEconomicalBiological testingFluorescence/phosphorescenceSolid phasesFluorescence
Glass thin slice with the multiple reaction ponds is set up on the outer casing of the compound chip for detecting oneself immunity antibody of diabetes. the detecting micro array is built in each pond. Four different kinds of protein solid phase detecting pieces in mini type with sample specification size constitute each detecting micro array. These detecting pieces are the piece of the islet cells, the piece of GAD protein, the PTP protein and the Insulin protein. First, these detecting pieces are prepared, then these pieces are assembled. The detecting method includes two steps: first, the indirect fluorescence method is used; then, the biochip laser co-focusing scanner determines the result. Comparing with the prior art the invention possesses the advantages of the accurate clinical analysis and the low cost.
Owner:XIAN BESTEDIT BIOMEDICAL SERVICES
High-resolution tomography optical microscopic imaging device for tissue samples
InactiveCN103207150AEnables tomographic micro-optical imagingHigh resolutionWithdrawing sample devicesMaterial analysis by optical meansMicroscopic imageTissue sample
The invention discloses a high-resolution tomography optical microscopic imaging device for tissue samples. The high-resolution tomography optical microscopic imaging device based on mechanical cutting can cut the tissue samples into thin slices and simultaneously carry out microscopic optical imaging for the thin slices. The device comprises a tissue sample cutting module and an optical microscopic imaging system. A cutting tool of the device is subjected to opacity treatment. The high-resolution tomography optical microscopic imaging device has the advantages that the slices are physically separated from remaining tissue samples in a cutting procedure while tomography images are acquired, illumination light or excitation light of the optical microscopic system cannot be transmitted through the thin slices and the cutting tool to illuminate or excite the remaining tissue samples below the cutting tool owing to the opacity treatment for the cutting tool, and accordingly interference of the tissue samples which are not cut currently on sampling for the slices is eliminated. The high-resolution tomography optical microscopic imaging device can be used for quick high-resolution tomography microscopic imaging for tissue samples.
Owner:HUAZHONG UNIV OF SCI & TECH
Human palm print image acquiring device and processing method
ActiveCN102521584AImprove extraction speedImprove recognition accuracyCharacter and pattern recognitionPalm printImaging quality
The invention provides a human palm print image acquiring device and a processing method. The human palm print image acquiring device consists of a synchronization unit, a sensor unit, a control unit and a computer. The synchronization unit is connected with the sensor unit. The sensor unit is connected with the control unit. The control unit is connected with the computer. The synchronization unit consists of a multiple-reflection optical system, a roller pair, a gear bank and a photoelectric encoder. The sensor unit comprises a CCD (Charge Coupled Device) linear array image sensor and a CIS (Contact Image Sensor) linear array image sensor module. The control unit is a linear array palm print image acquisition control unit on the basis of an FPGA (Field Programmable Gate Array) and a USB (Universal Serial Bus). According to the invention, under the condition of keeping the image quality and the identification precision, the volume is reduced. A palm print image acquired by the human palm print image acquiring device can be spliced into various resolutions such as 100dpi, 200dpi and the like to adapt to different requirements. According to the invention, the mobile palm print identification can be realized and meanwhile, the human palm print image acquiring device can also be used as a fixed palm print identification device. Two different functions can be achieved by one piece of equipment. The human palm print image acquiring device is flexible and is easy to use.
Owner:HARBIN INST OF TECH
Monoclonal antibody of hepatoma-derived growth factor and use thereof
InactiveCN101302254AEasy to identifyEffectively tested and verifiedImmunoglobulins against growth factorsMaterial analysisMonoclonal antibodyLiver cancer
The invention relates to anti-liver cancer derived growth factor monoclonal antibody and an application of the same. The invention makes the specific monoclonal antibody of anti-hepatoma derived growth factor (HDGF), wherein the monoclonal antibody can be used to make HDGF detection reagent suitable for a plurality of detection method.
Owner:曹伯良 +2
Method for detecting small molecule protein and peptides by using sandwich immunization sensing method
InactiveCN1480731AStrong optical signalPlay a role in biological amplificationBiological testingProtein insertionImmune complex deposition
The invention belongs to immunity detection area, especially related to method for quantitative measuring proteins and peptides with molecular weight under 20kDa. Gold-coated slide glass or triangular prism is as detection substrate. The method includes following procedures: forming an assembling body, forming duality immune complex of first antibody - antigen, forming sandwich ternary immune complex of first antibody - antigen - second antibody as well as resonant wavelength testing. The antigens to be detected are insulin bFGF, T3 or T4 etc. the first antibody and second antibody are first and second monoclone antibodies possessing specificities and complementarity for anti each antigen to be detected. The invention monitors dynamic reaction procedure of biomoleculars, and tests change of concentration of object to be detected with high sensitivity. The combination of second anti body generates stronger optical signal, creating biologic amplified activate.
Owner:JILIN UNIV
Human alpha-defensin peptide enzyme linked immunosorbent assay kit
InactiveCN104535771AStrong specificityOvercome the disadvantages that it is difficult to produce specific antibodies, etc.Biological testingBinding ratioAlpha defensin
The invention relates to a human alpha-defensin peptide enzyme linked immunosorbent assay kit and a preparation and detection method thereof. The human alpha-defensin peptide enzyme linked immunosorbent assay kit comprises a mouse-anti-human HNP monoclonal antibody coated ELISA plate, an HNP protein freeze-dried standard substance, a rabbit anti-human HNP polyclonal antibody, a horseradish peroxidase labeled goat anti-rabbit immune globulin polyclonal antibody, standard diluent, sample diluent, a 25-fold concentrated washing buffer solution, antibody diluent, a TMB developing solution and an enzyme reaction terminating solution. The preparation method comprises the following steps: coupling alpha-defensin and a carrier protein, adopting a coupling compound with the optimal coupling binding ratio (the molar ratio of HNP to SPDP to mouse serum albumin is 1:5:1, and the molar ratio of HNP to SPDP to rabbit serum albumin is 1:5:1, preparing to obtain a high-sensitivity antibody by using a complete antigen, and assembling the kit by using the obtained antibody. The kit disclosed by the invention has the advantages of high detection sensitivity, accurate result, wide detection range, simplicity and convenience in operation, long preservation time and the like.
Owner:WUHAN NCE BIOMEDICAL CO LTD
Qualitative and quantitative foot and mouth disease virus antigen detection method
InactiveCN105044344AImprove stabilityWill not affect test resultsMaterial analysisVaccine ProductionFoot mouth disease virus
The invention provides a western blotting luminescence and grey level combined foot and mouth disease virus antigen detection method. The method comprises the following steps: (1) SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gelelectrophoresis); (2) blotting transfer; (3) immune reaction; (4) developing or luminous reaction; (5) judgment of the property of an antigen to be detected according to a specific antigen band; (6) calculation of the content according to the luminous grey level of the antigen. The method can not only qualitatively but also quantitatively detect the foot and mouth disease virus antigen, has the characteristics of being safe, quick, simple, convenient, accurate and specific, realizes specific quantitative detection of various foot and mouth disease virus antigens, originates the mode of adopting western blotting luminescence to quantitatively detect the foot and mouth disease virus antigen, and has an important guiding meaning for vaccine production.
Owner:CHINA ANIMAL HUSBANDRY IND
Preparation of an electrolytic solution and use thereof for electrochemically detecting content of sanshool quickly
ActiveCN110501394AConductiveNo background distractionsMaterial electrochemical variablesElectrolytic agentSupporting electrolyte
The invention, which belongs to the technical field of detection in food, particularly relates to preparation of an electrolytic solution and the use thereof for electrochemically detecting the content of sanshool quickly. The method comprises: providing an electrolytic solution containing a polar solvent and a supporting electrolyte; extracting a sanshool mother solution of a sample, carrying outdilution by using the electrolytic solution to obtain a to-be-tested solution, and adding an alpha-SOH standard solution to obtain a mixed solution; preparing an equal-concentration equal-volume to-be-tested solution again and adding two-times-volume alpha-SOH standard solution to obtain a mixed solution; repeating the process by multiple times and then detecting differential pulse voltammetry curves of sanshool substances in the mixed solution respectively by using a three-electrode system; and calculating the content of sanshool by combining a formula. The electrolytic solution has conductivity and no background interference exists during detection. The electrolytic solution has advantages of fast detection, high sensitivity, simpleness, high measurement accuracy, short time required for sample analysis, low cost, and great convenience in on-line detection and the like in the specific detection process.
Owner:JIANGSU UNIV
Method for detecting telomere length
ActiveCN108486221ANo distractionHigh sensitivityMicrobiological testing/measurementFluorescenceFlow cytometry
The invention relates to a method for detecting telomere length. The method comprises the following steps: S1, pretreating a cell sample to be detected to obtain a M-phase chromosome suspension of thecell sample to be detected; S2, centrifuging the chromosome suspension, removing a supernatant to obtain a first precipitate containing M-phase chromosomes, mixing the first precipitate with a hybridization solution containing a telomere-specific peptide nucleic acid fluorescent probe, denaturing and hybridizing to obtain a suspension containing the hybrid chromosomes; S3, washing and centrifuging the hybrid chromosome suspension, removing a supernatant to obtain a second precipitate containing the hybrid chromosomes, suspending the second precipitate by using a suspending medium to obtain adetection sample suspension; S4, detecting fluorescent signals of the chromosomes in the detection sample suspension one by one in a single-particle level by an ultrahigh-sensitivity flow cytometry. By the method, fast, accurate and quantitative detection of the human telomere length and the short telomere ratio in a single-chromosome level can be achieved.
Owner:XIAMEN UNIV
Method for preparing oocyte paraffin cut film
InactiveCN100587452CUtensils are easy to getLow costPreparing sample for investigationBiological testingParaffin waxDirect effects
The invention provides a method for preparing a paraffin section of an oocyte. The method of the invention comprises the steps of fixing, dehydrating, transparency processing, paraffin embedding and slicing, etc., the method of the invention leads the oocyte to be transferred in the circumstance of liquid during the processes of fixing, dehydrating, transparency processing, etc. without a direct effect from outer force, thus the original morphosis of the oocyte can be maintained in maximum extent; a colorless transparent vessel is used for being filled with the oocyte, to lead the oocyte to beseen clearly; after the oocyte is transferred to a mould box, a xylene is removed to reveal the oocyte, thus the oocyte can be found more easily; before the paraffin embedding, a plurality of coloredgranules are scattered around the oocyte, thereby being convenient for the location of the oocyte in the paraffin. The method of the invention can make a paraffin section of a few or even a single oocyte of cursorial bird with the diameter of 100 microns to 600 microns, the structure of the gained section is clear and complete, and the staining effect is good.
Owner:CHINA AGRI UNIV
A high-resolution mass spectrometry imaging system image acquisition semiconductor thin film, preparation method and application
ActiveCN104597113BOvercome limitationsNo background distractionsMaterial nanotechnologyZinc oxides/hydroxidesResolution (mass spectrometry)Physical chemistry
Owner:HUAZHONG NORMAL UNIV
Preparation method of functionalized rare earth long-persistence nanocomposites and its application in latent fingerprint imaging
InactiveCN105295907BAchieve imagingFast wayDiagnostic recording/measuringSensorsLatent fingerprintFingerprint detection
The invention discloses a preparation method of a functionalized rare earth long-lasting nanocomposite material and its application in latent fingerprint imaging, belonging to the technical field of biological analysis and fingerprint detection. Avidin was modified to the surface of amino-functionalized strontium aluminate rare earth doped long-lasting nanomaterials to prepare avidin-functionalized rare-earth long-lasting nanocomposites, and then using the recognition function between biotin and avidin, the Avidinylated rare earth long-persistence nanocomposites attached to latent fingerprints labeled with biotinylated antibodies. The strontium aluminate rare earth doped long afterglow nanomaterial has long afterglow characteristics, and after being irradiated by a light source and removed, it still keeps emitting light, which can realize latent fingerprint imaging. The method of the invention is fast, simple and has no background interference.
Owner:NANCHANG UNIV
Monoclonal antibody of hepatoma-derived growth factor and use thereof
InactiveCN101302254BEasy to identifyEffectively tested and verifiedImmunoglobulins against growth factorsMaterial analysisMonoclonal antibodyLiver cancer
The invention relates to anti-liver cancer derived growth factor monoclonal antibody and an application of the same. The invention makes the specific monoclonal antibody of anti-hepatoma derived growth factor (HDGF), wherein the monoclonal antibody can be used to make HDGF detection reagent suitable for a plurality of detection method.
Owner:曹伯良 +2
Human palm print image acquiring device and processing method
ActiveCN102521584BImprove extraction speedImprove recognition accuracyCharacter and pattern recognitionPalm printImaging quality
The invention provides a human palm print image acquiring device and a processing method. The human palm print image acquiring device consists of a synchronization unit, a sensor unit, a control unit and a computer. The synchronization unit is connected with the sensor unit. The sensor unit is connected with the control unit. The control unit is connected with the computer. The synchronization unit consists of a multiple-reflection optical system, a roller pair, a gear bank and a photoelectric encoder. The sensor unit comprises a CCD (Charge Coupled Device) linear array image sensor and a CIS (Contact Image Sensor) linear array image sensor module. The control unit is a linear array palm print image acquisition control unit on the basis of an FPGA (Field Programmable Gate Array) and a USB (Universal Serial Bus). According to the invention, under the condition of keeping the image quality and the identification precision, the volume is reduced. A palm print image acquired by the human palm print image acquiring device can be spliced into various resolutions such as 100dpi, 200dpi and the like to adapt to different requirements. According to the invention, the mobile palm print identification can be realized and meanwhile, the human palm print image acquiring device can also be used as a fixed palm print identification device. Two different functions can be achieved by one piece of equipment. The human palm print image acquiring device is flexible and is easy to use.
Owner:HARBIN INST OF TECH
Preparation of electrolytic solution and its use in electrochemical rapid detection of pepperocannin content
ActiveCN110501394BLong analysis timeConductiveMaterial electrochemical variablesSupporting electrolyteElectrolytic agent
The invention belongs to the technical field of detection in food, and in particular relates to the preparation of an electrolytic solution and its use for electrochemical rapid detection of the content of xanthanin; the method is as follows: firstly, an electrolytic solution is provided, which is composed of a polar solvent and a supporting electrolyte ; Then extract the peppercornin mother liquor of the sample, dilute it with electrolyte to obtain the solution to be tested, add α-SOH standard solution, and obtain a mixed solution; reconfigure the solution to be tested with equal concentration and volume, and add 2 times the volume of α-SOH standard solution , to obtain the mixed solution; repeating this many times, finally using the three-electrode system to detect the differential pulse voltammetry curve of the pepperonamide substance in the mixed solution respectively; There is no background interference during detection; in the specific detection process, it has the advantages of fast detection, sensitivity, simplicity, high measurement accuracy, short sample analysis time, low cost, and convenient online detection.
Owner:JIANGSU UNIV
Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof
InactiveCN1176377CHas clinical practical valueReduce testing costsBiological testingFluorescence/phosphorescenceDiabetes mellitusFluorescence
Glass thin slice with the multiple reaction ponds is set up on the outer casing of the compound chip for detecting oneself immunity antibody of diabetes. the detecting micro array is built in each pond. Four different kinds of protein solid phase detecting pieces in mini type with sample specification size constitute each detecting micro array. These detecting pieces are the piece of the islet cells, the piece of GAD protein, the PTP protein and the Insulin protein. First, these detecting pieces are prepared, then these pieces are assembled. The detecting method includes two steps: first, the indirect fluorescence method is used; then, the biochip laser co-focusing scanner determines the result. Comparing with the prior art the invention possesses the advantages of the accurate clinical analysis and the low cost.
Owner:XIAN BESTEDIT BIOMEDICAL SERVICES
Micro-plasma excitation source and excitation method based on ultrasonic atomization sample introduction
ActiveCN113720811AReduce the temperatureImprove detection accuracyAnalysis by material excitationPlasma techniqueAerosolizeAlternating current
The invention provides a micro-plasma excitation source based on ultrasonic atomization sample introduction, and relates to the field of emission spectrum element analysis excitation light sources. The micro-plasma excitation source comprises an AC power supply, two metal electrodes and an ultrasonic atomization sheet; the AC power supply is electrically connected with one end of each metal electrode; the discharge ends of the metal electrodes are positioned in the same plane; the ultrasonic atomization sheet is horizontally arranged above the discharge ends of the metal electrodes and is used for converting a solution to be detected into aerosol and downwards spraying the aerosol to the discharge ends of the metal electrodes and space between the metal electrodes; and the AC power supply, the metal electrodes and the aerosol are matched to form a V-shaped micro plasma below the metal electrodes. The invention further provides an excitation method adopting the micro-plasma excitation source based on ultrasonic atomization sample introduction, and the detection precision of metal elements can be effectively improved.
Owner:CHINA UNIV OF GEOSCIENCES (WUHAN)
Method for detection of residual organic matter in fire scene based on surface-enhanced Raman spectroscopy
ActiveCN105424677BEasy to operateSimple structureRaman scatteringSurface-enhanced Raman spectroscopyFiltration
The invention relates to a method for detecting residual organics on a fire scene based on a surface enhancing raman spectrum. The method comprises the following steps: constructing a detection substrate material which comprises a metal film and a nanometer particle, wherein the surface of the metal film is modified with functional groups, the metal film has a hydrophobic effect and is smooth and flat, and the nanometer particle has surface enhancing raman activity and is free from modification with the functional groups; dropwise adding the filtration liquid onto the surface of the metal film with the hydrophobic effect; after finishing the dropwise adding, naturally volatilizing the liquid; dropwise adding the colloid containing the nanometer particle with the surface enhancing activity on the surface of the metal film and utilizing a raman spectrometer to detect the colloid on the surface of the metal film; comparing and analyzing the acquired spectrum chart and identifying the category of the organic compound in the to-be-detected article. According to the method, the operation is simple and convenient; the detecting base structure is simple and can be directly used for extracting, absorbing and enriching the analyte in the solution sample; the designed and constructed enhancing system can realizably acquire high-quality SERS test result; the method has the advantages of high sensitivity, excellent repeatability, zero background interference, and the like.
Owner:SHANGHAI FIRE RES INST OF THE MIN OF PUBLIC SECURITY +1
Method for matching vehicle speed based on linear array CCD image
InactiveCN101206229BThe speed measurement method works wellHigh resolutionRoad vehicles traffic controlDevices using time traversedImage resolutionTime difference
Owner:CHANGAN UNIV +2
A traffic information acquisition system based on linear array CCD video camera imaging
InactiveCN100592350CThe speed measurement method works wellHigh resolutionDetection of traffic movementClosed circuit television systemsSplit linesTraffic flow
The invention discloses a traffic information acquiring system based on linear CCD imaging, which comprises two linear CCD cameras spaced at a certain interval and disposed on a support above two roadsections, each is provided with an auxiliary lighting headlight; and a plurality of auxiliary planar array cameras arranged at the position 15 m away from the front of the two linear CCD cameras andrespectively located on the middle of a driveway and on the separating line of the driveway, wherein the auxiliary planar array cameras in the middle of the driveway can shoot vehicles passing the road, and the auxiliary planar array cameras in the driveway separating line can perform panning shot. When the vehicles pass the road section, the linear CCD cameras can acquire linear image data of thevehicles passing the road section; the acquired linear image data is processed and analyzed to obtain traffic parameters such as traffic flow, vehicle velocity, traffic stream density and road occupation rate, and the planar array cameras can shoot vehicle image which is analyzed and processed to obtain license plate information.
Owner:CHANGAN UNIV
A method for in situ detection of small molecular compounds entering mitochondria in cells
ActiveCN106770611BTruly reflect completenessResponse integrityMaterial analysis by electric/magnetic meansMass spectrometryCytoplasmic protein
The invention discloses a method for detecting entry of small molecule compounds into mitochondria at a cell in-situ level. The method particularly comprises the following steps: selecting suitable small molecule compounds to be incubated with cells; acquiring mitochondria with complete physiological structures without destroying cell mitochondria; collecting cytoplasm; incubating with the small molecule compounds and the acquired mitochondria; respectively pyrolyzing the obtained mitochondria after incubating the small molecule compounds and the cytoplasm collected before under non-denaturing conditions to acquire a mitochondrial protein lysate and a cytoplasmic protein lysate; and then, detecting whether the small molecule compounds enter the mitochondria at the cell in-situ level by combining with mass spectrum analysis. The method can be adopted to detect whether the small molecule compounds enter the mitochondria at the cell in-situ level, and can further detect a way in which the small molecule compounds enter the mitochondria, can be directly applied to target region verification of a pilot drug or screening of the pilot drug using the mitochondrion as an effector, and has the advantages of high reliability and high sensitivity.
Owner:江西海普洛斯医学检验实验室有限公司
Preparation of near-infrared light-emitting Yb and Tm co-doped core-shell-shell structure nanocrystal and application of nanocrystal in immunochromatography detection
ActiveCN113980682AInhibition of cross-relaxation quenchingEnhanced near-infrared luminous intensityMaterial nanotechnologyNanoopticsDiseaseLuminous intensity
The invention belongs to the technical field of immunological detection, and particularly relates to preparation of a near-infrared light-emitting Yb and Tm co-doped core-shell structure nanocrystal and application of the nanocrystal in immunochromatographic detection. A synthesis scheme of the core-shell structure nanocrystal is adopted, by means of the scheme, sensitizing ions Yb can be highly doped to 98%, and therefore, the absorption capacity of the nanocrystal to 980nm light is improved; meanwhile, the doping proportion of luminous ions Tm ions in the middle shell layer is determined to be 2%. Under the proportion, a cross relaxation quenching phenomenon and the balance of effective energy transfer between Yb and Tm are realized, and finally, the near-infrared luminous intensity of the core-shell structure nanocrystal is greatly improved (the luminous intensity is improved by 850 times compared with that of standard Yb and Tm bare core nanocrystals). An immunochromatography detection method is established by using the high-brightness near-infrared light-emitting nanocrystal as an immunofluorescence probe, thereby realizing the immunochromatography method for detecting disease indexes without background interference and with high sensitivity.
Owner:THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV +1
A silicon nanowire array chip method for detecting cell metabolites and lipids
ActiveCN110530965BAvoid background peakingNo background distractionsMaterial analysis by electric/magnetic meansSemiconductor/solid-state device manufacturingEtchingCell metabolite
The invention discloses a method for detecting cell metabolites and lipids by a silicon nanowire array chip. The invention obtains a silicon nanowire array chip with multiple sites through photolithographic patterning and metal-assisted etching, and then in multiple surface modification on the basis of silicon nanowire array chip at the site; the obtained chip integrates superior desorption ability, ionization ability and high-throughput detection ability of matrix-free mass spectrometry, which simplifies the pretreatment and processing of complex samples. The detection process; and by establishing a spectrogram analysis model, the method can simultaneously detect the cell subtypes of single cell samples, mixed cell samples, xenograft tumor tissue samples, and cell samples after drug action. The operation is simple and the application range is wide.
Owner:HANGZHOU WELL HEALTHCARE TECH CO LTD
Methods for measuring telomere length
ActiveCN108486221BNo distractionHigh sensitivityMicrobiological testing/measurementCytometryGenomic clone
The present disclosure relates to a method for detecting telomere length, the method comprising the following steps: S1. Pretreating the cell sample to be tested to obtain the M phase chromosome suspension of the cell sample to be tested; S2. Centrifuging the chromosome suspension and removing the supernatant to obtain the first precipitate containing chromosomes in the M phase, mixing the first precipitate with a hybridization solution containing a telomere-specific peptide nucleic acid fluorescent probe, performing denaturation and hybridization to obtain a suspension containing hybrid chromosomes; S3. Washing, centrifuging, and removing the supernatant of the hybrid chromosome suspension to obtain a second precipitate containing the hybrid chromosome, suspending the second precipitate with a suspension medium to obtain a sample loading suspension; S4. Using ultra-high sensitivity flow Cytometry detects the fluorescent signals of the chromosomes in the detection loading suspension one by one at the single particle level. The method can realize rapid, accurate and quantitative detection of human telomere length and short telomere ratio at the single-chromosome level.
Owner:XIAMEN UNIV
A rapid and sensitive method for the detection of small RNAs
InactiveCN104232772BHigh sensitivityNo background distractionsMicrobiological testing/measurementCelluloseBiotin
The invention discloses a method for quickly and sensitively detecting small RNA, belonging to the field of molecular biology, comprising the following steps: (1) extracting and purifying the small RNA to be detected; (2) probe design: the probe uses biotin or ground (3) liquid-phase hybridization: hybridize the small molecule RNA to be detected with the probe in the buffer, and use exonuclease to excise the unhybridized excess single strand and then perform gel electrophoresis; (4) Membrane transfer: transfer the hybridization product on the gel after electrophoresis to a solid support (such as nylon membrane, cellulose membrane); (5) Antibody incubation: transfer the hybridization product obtained in step (4) The solid phase support and the specific antibody are incubated for hybridization; (6) signal detection. The method can detect at least 0.005 fmol of miRNA, and the sensitivity is at least 10 times higher than that of the prior art. The method has a fast detection speed and can realize high-throughput detection and quantitative detection.
Owner:SICHUAN UNIV
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