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Preparation of near-infrared light-emitting Yb and Tm co-doped core-shell-shell structure nanocrystal and application of nanocrystal in immunochromatography detection

A near-infrared, co-doping technology, applied in the field of immunological detection, can solve the problems of cross-relaxation fluorescence quenching, and the ability to absorb 980nm photons cannot be significantly improved.

Active Publication Date: 2022-01-28
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the high doping of the sensitizing ion Yb of the core-shell nanocrystals used in this patent can easily cause cross-relaxation fluorescence quenching, resulting in no significant improvement in its ability to absorb 980nm photons.

Method used

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  • Preparation of near-infrared light-emitting Yb and Tm co-doped core-shell-shell structure nanocrystal and application of nanocrystal in immunochromatography detection
  • Preparation of near-infrared light-emitting Yb and Tm co-doped core-shell-shell structure nanocrystal and application of nanocrystal in immunochromatography detection
  • Preparation of near-infrared light-emitting Yb and Tm co-doped core-shell-shell structure nanocrystal and application of nanocrystal in immunochromatography detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Near-infrared luminescent Yb and Tm co-doped core-shell structure nanocrystals (NaYF 4 @NaF 4 :Yb98%,Tm2%@NaYF 4 Preparation of core-shell-shell nanocrystals)

[0051] 1. Preparation of nuclear NaYF 4

[0052] 1) First add 7.5mL oleic acid (OA) and 15mL octadecene (ODE) into a three-necked flask, then add 1mmol of YAc 3 , 4H 2 O (yttrium acetate tetrahydrate), then heated to 160 ° C for 120 minutes to completely dissolve to remove moisture and oxygen, and finally cooled to room temperature to obtain a mixed solution;

[0053] 2) Dissolve 10mL containing NH 4 Add the methanol solution of F (1mmoL) and NaOH (1mmoL) to the mixture in the above-mentioned three-necked flask, and heat to 120°C for 30min to remove methanol; then pass a sufficient amount of nitrogen and heat the mixture to 300°C for 60min;

[0054] 3) After the reaction, the obtained liquid was naturally cooled to below 100° and transferred to a centrifuge tube, 5 mL of absolute ethanol was add...

Embodiment 2

[0073] Example 2 Preparation of lateral flow test strips based on near-infrared excitation and emission for luminescent detection of serum markers

[0074] The lateral flow test strip of the present embodiment is a lateral flow test strip using the NaYF4@NaYF4:Yb, Tm@NaYF4 core-shell structure nanocrystal of embodiment 1 as an immunolabeled lateral flow test strip, which consists of NaYF4@NaYF4 : Yb, Tm@NaYF4 core-shell structure nanocrystal, PVC support backplane, detection pad, binding pad, sample pad, water-absorbing pad (purchased by whatman company);

[0075] Among them, the detection pad is composed of a nitrocellulose membrane (nitrocellulose membrane) and a detection line and a control line drawn in parallel on the nitrocellulose membrane; the detection line is a line drawn with 1-2 mg / mL serum index antibody, and the line The width is 1-1.2 mm, and the volume of dropping is 1-1.2 L / cm; the control line is a line drawn with 1-2 mg / mL goat anti-chicken IgY antibody solu...

Embodiment 3

[0092] Example 3 Detection Limit of Lateral Flow Test Strip Based on Near-infrared Excitation and Emission of Serum Marker Luminescent Detection

[0093] Explore the detection limit of the lateral flow test strip prepared in Example 2 to measure the SARS-CoV-2 RBD-IgG antibody, the specific method is as follows:

[0094] 1) SARS-CoV-2RBD-IgG antibody standard (purchased from Jinan Protein Technology Co., Ltd.) was added to the serum sample without SARS-CoV-2RBD-IgG antibody before the epidemic to prepare SARS-CoV-2RBD-IgG A series of serum samples with antibody concentrations of 0ug / mL, 0.05ug / mL, 0.1ug / mL, 1ug / mL, 5ug / mL, 10ug / mL, and 20ug / mL;

[0095] 2) Add 20 microliters of serum samples of each concentration to the sample hole of the test paper card (the lateral flow test strip of Example 2), and then add 80 microliters of sample Tris buffer [20mM Tris-base, 0.85% (w / v ) NaCl, 0.5% (w / v) TX-100, 0.1% (v / v) Tween-20, 0.1% (w / v) S9, PH 8.0]; Invention patent 202021494870....

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Abstract

The invention belongs to the technical field of immunological detection, and particularly relates to preparation of a near-infrared light-emitting Yb and Tm co-doped core-shell structure nanocrystal and application of the nanocrystal in immunochromatographic detection. A synthesis scheme of the core-shell structure nanocrystal is adopted, by means of the scheme, sensitizing ions Yb can be highly doped to 98%, and therefore, the absorption capacity of the nanocrystal to 980nm light is improved; meanwhile, the doping proportion of luminous ions Tm ions in the middle shell layer is determined to be 2%. Under the proportion, a cross relaxation quenching phenomenon and the balance of effective energy transfer between Yb and Tm are realized, and finally, the near-infrared luminous intensity of the core-shell structure nanocrystal is greatly improved (the luminous intensity is improved by 850 times compared with that of standard Yb and Tm bare core nanocrystals). An immunochromatography detection method is established by using the high-brightness near-infrared light-emitting nanocrystal as an immunofluorescence probe, thereby realizing the immunochromatography method for detecting disease indexes without background interference and with high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of immunological detection, and in particular relates to the preparation of a near-infrared luminescent Yb and Tm co-doped core-shell-shell nanocrystal and its application in immunochromatographic detection. Background technique [0002] In recent years, with the continuous development of mobile medicine and home medicine, researchers have been seeking new types of luminescent materials as luminescent probes to achieve high sensitivity and high precision in the determination of various disease markers in serum, including myocardial infarction markers (CTNI and MYO); bacterial and viral infection markers (CRP, SAA, and PCT); diabetes markers (glycosylated hemoglobin); preoperative infectious disease markers (HIV, syphilis, hepatitis B and hepatitis C) and sudden infectious diseases, etc. Among them, the detection method based on gold nanoparticles has developed rapidly, and has been rapidly applied in the fie...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02G01N33/558B82Y20/00B82Y30/00
CPCC09K11/02C09K11/7705G01N33/558B82Y20/00B82Y30/00Y02A50/30
Inventor 冀天星魏嵬
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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