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Microorganism concentration process and device

一种浓集、聚合物的技术，应用在捕获或浓集微生物领域，能够解决昂贵、速度慢等问题，达到简单成本、低成本、高抗阻塞性的效果

Active Publication Date: 2013-07-24
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such methods tend to be expensive and are still somewhat slower than desired for at least some diagnostic applications

Method used

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  • Microorganism concentration process and device
  • Microorganism concentration process and device
  • Microorganism concentration process and device

Examples

Experimental program
Comparison scheme
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example

[0129] Objects and advantages of this invention are further illustrated by the following examples, but the particular materials and amounts thereof recited in these examples, as well as other conditions and details, should not be construed to unduly limit this invention. All parts, percentages, ratios, etc. in the following examples are by weight unless otherwise indicated. Solvents and other reagents were obtained from Sigma-Aldrich Chemical Company, Milwaukee, WI unless otherwise noted. All microbial cultures were purchased from the American Type Culture Collection (ATCC; Manassas, VA) in Manassas, VA. Unless otherwise indicated, experimental results are the average of 2 tests. Overnight cultures were prepared by streaking selected microorganisms onto tryptic soy agar plates followed by overnight incubation at 37°C. All microbial counts were performed according to standard microbiological enumeration methods for colony forming units and counts are approximate.

[0130] ...

example 1-17 and comparative example C1-C4

[0178] Examples 1-17 and Comparative Examples C1-C4: Preparation of concentration devices 1-17 and C1-C4

[0179] Fiber premixes were prepared having the fiber and water compositions shown in Table 1. Each fiber premix was prepared by first mixing the fibrillated fiber (fiber 1 or fiber 2) with the indicated amount of tap water in a 4L blender ((Waring Commercial Heavy Duty Blender, Model 37BL84) ) blended at medium speed for 90 seconds. Check that the fibers proceed to ensure they are evenly dispersed without knots or clumps, and blend them further if necessary to break up any clumps. Then add the other indicated fibers Further blending was carried out and the indicated amount of premix was added to a stainless steel beaker and an impeller mixer (Stedfast Stirrer Model SL2400, Fisher Scientific, available from VWR, West Chester, Pennsylvania (VWR, West Chester, PA)) was mixed at speed setting 4 for five minutes. When used, the latex binder was dispersed in approximately 25...

example 16

[0183] Example 16 was prepared essentially by the procedure described above, except that AS-talc particles were added to the fiber premix prior to the addition of the latex binder.

[0184] The resulting porous fibrous nonwoven matrix was sealed in a plastic bag and irradiated with 0.5 kGy / hr of gamma radiation at a total dose of 4 kGy for 8 hours, or autoclaved at 121° C. for 15 minutes to sterilize the matrix. Square sample matrices (measured 20 cm x 20 cm (8 in x 8 in)) were cut from the matrices of the selected examples, weighed and solids retention values ​​were determined for these sample matrices, as shown in Table 2 out.

[0185]

[0186] Table 2 .

[0187] instance number

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PUM

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Abstract

A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.

Description

technical field [0001] The present invention relates to methods for capturing or concentrating microorganisms so that they remain viable for detection or assay. In other aspects, the invention also relates to concentration devices (and diagnostic kits comprising these devices) for carrying out such methods and to methods for the manufacture of the devices. Background technique [0002] Foodborne illnesses and hospital-acquired infections caused by microbial contamination are a concern in many parts of the world. Accordingly, it is often desirable or necessary to assay various clinical samples, food samples, environmental samples, or other samples for the presence of bacteria or other microorganisms to determine the type and / or amount of microorganisms present. [0003] For example, bacterial DNA or bacterial RNA can be analyzed to assess the presence or absence of a particular bacterial species even in the presence of other bacterial species. However, the ability to detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12Q1/06C12M1/16C12M1/34
CPCC12Q1/04C12M47/02G01N1/405G01N33/84C12Q1/24G01N1/40G01N33/56911G01N2001/4088G01N2035/00475
Inventor M·T·克舍撒加A·W·雷宾斯
Owner 3M INNOVATIVE PROPERTIES CO
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