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Chrysanthemum simplified tissue culturing method

A tissue culture, chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting general promotion to the market, long lighting cultivation, increasing costs, etc., to achieve good theoretical research value, good production and application. Prospects, the effect of reducing production costs

Inactive Publication Date: 2013-09-04
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Judging from the current rooting status of plant tissue rapid propagation technology, most of them use carbohydrates in the culture medium as energy sources. In a constant temperature, constant light intensity, closed and sterile environment, a wide variety of drugs are used to prepare the culture medium and the price is relatively high. , the cultivation process requires fine equipment, and the whole process consumes a lot of electricity, especially the long lighting cultivation increases the cost
After rooting, the test-tube seedlings are transplanted in the outdoor environmental conditions with strong light, variable temperature and many bacteria through hardening under the superior sterile environment conditions. The price of ordinary seedlings is several times or dozens of times higher, which limits the general promotion of this technology in the majority of grassroots units and makes more plant tissue culture varieties go to the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] For tissue culture in this example, a special tissue culture room is required, the temperature is controlled by an air conditioner to (25±1)°C, and the light intensity is 3000-4000 lx by using an incandescent lamp every day for 8 hours and 16 hours in the dark. The culture medium and various utensils required for inoculation were put into a pressure cooker for sterilization, and after natural cooling, they were taken out and put into the inoculation room for use. Take MS as the basic medium, sucrose 30g / L, agar 6g / L, pH value adjusted to 5.8, additional 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, medium at 121 ℃ Sterilize at high temperature for 16 minutes for use; select robust shoots of the current year, and use the lateral buds in the middle of the full but ungerminated shoots as explants. Remove the leaves from the branches, cut them into 2-3cm pieces with side buds, wash them with clean water, and then dry them with filter paper. First, disinfect with 75% alcohol for 20s u...

Embodiment 2

[0021] For tissue culture in this example, a special tissue culture room is required, the temperature is controlled by an air conditioner to (25±1)°C, and the light intensity is 3000-4000 lx by using an incandescent lamp every day for 8 hours and 16 hours in the dark. The culture medium and various utensils required for inoculation were put into a pressure cooker for sterilization, and after natural cooling, they were taken out and put into the inoculation room for use. Take MS as the basic medium, sucrose 30g / L, agar 6g / L, pH value adjusted to 5.8, additional 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, medium at 121 ℃ Sterilize at high temperature for 16 minutes for use; select robust shoots of the current year, and use the lateral buds in the middle of the full but ungerminated shoots as explants. Remove the leaves from the branches, cut them into 2-3cm pieces with side buds, wash them with clean water, and then dry them with filter paper. First, disinfect with 75% alcohol for 20s u...

Embodiment 3

[0023] For tissue culture in this example, a special tissue culture room is required, the temperature is controlled by an air conditioner to (25±1)°C, and an incandescent lamp is used to illuminate for 8 hours a day, dark for 16 hours, and the light intensity is 3000-4000 lx. The culture medium and various utensils required for inoculation were put into a pressure cooker for sterilization, and after natural cooling, they were taken out and put into the inoculation room for use. Take MS as the basic medium, sucrose 30g / L, agar 6g / L, pH value adjusted to 5.8, additional 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, medium at 121 ℃ Sterilize at high temperature for 16 minutes for use; select robust shoots of the current year, and use the lateral buds in the middle of the full but ungerminated shoots as explants. Remove the leaves from the branches, cut them into 2-3cm pieces with side buds, wash them with clean water, and then dry them with filter paper. First, disinfect with 75% alcohol f...

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Abstract

The invention discloses a chrysanthemum simplified tissue culturing method which comprises the following steps of: 1, carrying out disinfection treatment on an explant under an aseptic condition by taking a chrysanthemum stem with buds as the explant; 2, regenerating an adventitious bud in a culture medium in which a growth regulating agent is added by the stem; and 3, culturing by applying a filter paper bridge rooting technique: embedding two pressing bars respectively in the middle of two edge walls of a culturing box for placing filter paper bars, folding a piece of filter paper from the middle, selecting treated tissue culture seedlings, and inserting the lower parts of the tissue culture seedlings into the piece of filter paper, pouring clean water in the box so as to enable the piece of filter paper to absorb for the growth of seedlings. The chrysanthemum simplified tissue culturing method provided by the invention has no difficulty in cleaning agar, saves a great amount of labor force and resources, especially saves a great amount of medicaments and electricity charge, improves the rooting rate, is good in rooting quality, and is rich in rooting number; seedlings can be transplanted into soil without hardening-seedling, and the survival rate of transplanting is high; the remote transferring is convenient, and the tissue culture seedlings are hardly damaged; the investment of culture liquids is extremely low, but the effects are relatively obviously; and the method provided by the invention is especially suitable for culturing shuaiqi chrysanthemum.

Description

technical field [0001] The invention relates to a method for tissue culture of chrysanthemum, in particular to a method for tissue culture of Shuaiqi chrysanthemum. Background technique [0002] Shuaiqi is one of the top ten famous traditional chrysanthemums in my country. . The traditional methods of asexual reproduction such as cuttings, divisions, and grafting make plants seriously infected with viruses, slow in reproduction, weak in growth potential, and degenerate, making it difficult to cultivate and unable to meet market demand. For a long time, there is no effective method for plant virus disease control. With the development of modern biotechnology, it has been found that the apical meristem of plants is not infected by viruses, so it is expected that virus-free materials can be obtained by culturing the shoot tips. At present, a large number of tissue culture work of chrysanthemum has been carried out at home and abroad, but there is no report on the tissue cultu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张演义张秀省吕福堂于守超赵燕任秋萍邢柱东
Owner LIAOCHENG UNIV
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