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Chrysanthemum simplified tissue culturing method

A tissue culture, chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting general promotion to the market, long lighting cultivation, and low survival rate of transplanting, and achieves good production and application prospects. Good theoretical research value, the effect of simplifying the tissue culture process

Inactive Publication Date: 2015-07-01
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Judging from the current rooting status of plant tissue rapid propagation technology, most of them use carbohydrates in the culture medium as energy sources. In a constant temperature, constant light intensity, closed and sterile environment, a wide variety of drugs are used to prepare the culture medium and the price is relatively high. , the cultivation process requires fine equipment, and the whole process consumes a lot of electricity, especially the long lighting cultivation increases the cost
After rooting, the test-tube seedlings are transplanted in the outdoor environmental conditions with strong light, variable temperature and many bacteria through hardening under the superior sterile environment conditions. The price of ordinary seedlings is several times or dozens of times higher, which limits the general promotion of this technology in the majority of grassroots units and makes more plant tissue culture varieties go to the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] For tissue culture in this example, a special culture room for tissue culture is required, the temperature is controlled by an air conditioner at (25±1)°C, and the light intensity is 3000-4000 lx for 16 hours with an incandescent lamp for 8 hours a day. The medium and various utensils required for inoculation were sterilized in a pressure cooker, and after natural cooling, they were taken out and placed in the inoculation room for later use. Use MS as the basic medium, sucrose 30g / L, agar 6g / L, adjust the pH value to 5.8, add 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, and culture medium at 121℃ Sterilize under high temperature for 16 minutes for later use; select robust shoots of the year, and use plump but ungerminated lateral buds in the middle of the shoots as explants. Remove the leaves from the branches, cut them into 2-3cm small sections with lateral buds, wash them with clean water, and then dry them with filter paper. Sterilize with 75% alcohol for 20 seconds under ase...

Embodiment 2

[0021] For tissue culture in this example, a special culture room for tissue culture is required, the temperature is controlled by an air conditioner at (25±1)°C, and the light intensity is 3000-4000 lx for 16 hours with an incandescent lamp for 8 hours a day. The medium and various utensils required for inoculation were sterilized in a pressure cooker, and after natural cooling, they were taken out and placed in the inoculation room for later use. Use MS as the basic medium, sucrose 30g / L, agar 6g / L, adjust the pH value to 5.8, add 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, and culture medium at 121℃ Sterilize under high temperature for 16 minutes for later use; select robust shoots of the year, and use plump but ungerminated lateral buds in the middle of the shoots as explants. Remove the leaves from the branches, cut them into 2-3cm small sections with lateral buds, wash them with clean water, and then dry them with filter paper. Sterilize with 75% alcohol for 20 seconds under ase...

Embodiment 3

[0023] For tissue culture in this example, a special culture room for tissue culture is required, the temperature is controlled by an air conditioner at (25±1)°C, and an incandescent lamp is used for 8 hours of light and 16 hours of darkness every day, with a light intensity of 3000-4000 lx. The medium and various utensils required for inoculation were sterilized in a pressure cooker, and after natural cooling, they were taken out and placed in the inoculation room for later use. Use MS as the basic medium, sucrose 30g / L, agar 6g / L, adjust the pH value to 5.8, add 6-BA0.3mg / L+NAA0.3mg / L+IBA0.1mg / L, and culture medium at 121℃ Sterilize under high temperature for 16 minutes for later use; select robust shoots of the year, and use plump but ungerminated lateral buds in the middle of the shoots as explants. Remove the leaves from the branches, cut them into 2-3cm small sections with lateral buds, wash them with clean water, and then dry them with filter paper. Sterilize with 75% ...

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Abstract

The invention discloses a chrysanthemum simplified tissue culturing method which comprises the following steps of: 1, carrying out disinfection treatment on an explant under an aseptic condition by taking a chrysanthemum stem with buds as the explant; 2, regenerating an adventitious bud in a culture medium in which a growth regulating agent is added by the stem; and 3, culturing by applying a filter paper bridge rooting technique: embedding two pressing bars respectively in the middle of two edge walls of a culturing box for placing filter paper bars, folding a piece of filter paper from the middle, selecting treated tissue culture seedlings, and inserting the lower parts of the tissue culture seedlings into the piece of filter paper, pouring clean water in the box so as to enable the piece of filter paper to absorb for the growth of seedlings. The chrysanthemum simplified tissue culturing method provided by the invention has no difficulty in cleaning agar, saves a great amount of labor force and resources, especially saves a great amount of medicaments and electricity charge, improves the rooting rate, is good in rooting quality, and is rich in rooting number; seedlings can be transplanted into soil without hardening-seedling, and the survival rate of transplanting is high; the remote transferring is convenient, and the tissue culture seedlings are hardly damaged; the investment of culture liquids is extremely low, but the effects are relatively obviously; and the method provided by the invention is especially suitable for culturing shuaiqi chrysanthemum.

Description

technical field [0001] The invention relates to a tissue culture method of chrysanthemum, in particular to a tissue culture method of the Shuaiqi chrysanthemum variety. Background technique [0002] Shuaiqi is one of the top ten traditional chrysanthemums in my country. It has a large and plump flower shape with a single round, vermilion on the front and mud gold on the back. The petals are wide and inch wide and magnificent. . Traditional asexual reproduction methods such as cutting, branching, and grafting make the plants seriously infected with viruses, slow in reproduction, weak in growth, and degenerate, making it difficult to cultivate and unable to meet market demand. For a long time, there is no effective method for the prevention and treatment of plant virus diseases. With the development of modern biotechnology, it is found that the apical meristems of plants are not infected by viruses, so it is expected to obtain virus-free materials by using shoot tip culture. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张演义张秀省吕福堂于守超赵燕任秋萍邢柱东
Owner LIAOCHENG UNIV
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