A method for fixing CO2 by using Pseudocladon algae
A pseudocladon, CO2 technology, applied in separation methods, chemical instruments and methods, dispersed particle separation, etc., can solve problems affecting the health of workers, reduce work efficiency, endanger life and health, etc., and achieve permanent fixation and storage , easy operation, and the effect of reducing harm
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Embodiment 1
[0042] In this embodiment, a method for fixing CO by using Pseudocladon algae biological calcification is provided. 2 method, including the following steps:
[0043] Step 1: Isolation, cultivation and domestication of Pseudocladoma.
[0044] Collect samples of the algae community with Pseudocladonium, cultivate them in BG11 liquid medium, gently peel off and separate the algae filaments with tweezers, separate the pseudocladonium filaments, rinse them repeatedly with sterile water, and transfer them to sterilized BG11 liquid culture medium cultured in the base.
[0045] Pseudocladonium should be domesticated with a medium close to the state of biological calcified water before use. The medium for domesticating Pseudocladonium is an oligotrophic liquid medium with a composition of 5.9mg / LCa (NO 3 ) 2 4H 2 O,46.7mg / LNaNO 3 ,4.1mg / LK 2 HPO 4 ·3H 2 O,2.5mg / LMgSO 4 ·7H 2 O,168mg / LNaHCO 3 ,11.45mg / LNa-EDTA (disodium ethylenediaminetetraacetate), 3mg / LFeSO 4 ·7H 2 O,248μ...
Embodiment 2
[0053] In this embodiment, a method for fixing CO by using Pseudocladon algae biological calcification is provided. 2 method, including the following steps:
[0054] After separating, cultivating and domesticating Pseudomonas algae, the Pseudomonas algae cell fluid in the logarithmic growth phase (concentration of 1 × 10 5 cells) into a flat-bottomed glass bottle (volume 1.2L), under continuous light conditions (illuminance 4000lx), add a certain concentration of NaHCO to the algae cell liquid 3 solution, and then add a certain concentration of CaCl to the solution 2 Solution, so that the calcium ion concentration in the flat-bottomed glass bottle is 190mg / L, keep the temperature at about 20°C, and the initial pH value of the solution is 7.5-9, and continuously pass through the solution containing CO 2 gas (CO 2 The mass concentration is 0.4%), the gas flow rate is 30ml / min, and the process continues for 100h.
[0055] After the calcification process is over, filter the ab...
Embodiment 3
[0061] In this embodiment, a method for fixing CO by using Pseudocladon algae biological calcification is provided. 2 method, including the following steps:
[0062] After separating, cultivating and domesticating Pseudomonas algae, the Pseudomonas algae cell fluid in the logarithmic growth phase (concentration of 1 × 10 8 cells) into a flat-bottomed glass bottle (volume 1.2L), under continuous light conditions (illuminance 5000lx), add a certain concentration of NaHCO to the algae cell liquid 3 solution, and then add a certain concentration of CaCl to the solution 2 Solution, so that the calcium ion concentration in the flat-bottomed glass bottle is 150mg / L, keep the temperature at about 25°C, and the initial pH value of the solution is 7.5-9, and continuously pass through the solution containing CO 2 gas (CO 2 The mass concentration is 0.4%), the gas flow rate is 30ml / min, and the process continues for 100h.
[0063] After the calcification process is over, filter the ab...
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