Elastin-like polypeptide based temperature-sensitive polymer molecule brush and preparation method thereof

Abstract

The invention relates to an elastin-like polypeptide based temperature-sensitive polymer molecule brush and a preparation method thereof. The structural formula of the molecule brush is shown in the specification, and n in the formula is 100-500. The elastin-like polypeptide based temperature-sensitive polymer molecule brush obtained in the invention has a narrow phase transition range (-3DEG C), and the phase transition temperature of the molecule brush can be flexibly controlled through pH, the salt kind and the concentration, so the molecule brush can be used in the medicine controlled release filed, the catalysis field, the intelligent interface field, the protein separating and purifying field, the temperature inductor field and the like.

Description

Technical field [0001] The present invention involves a type of temperature -sensitive polymer molecular brush and its preparation methods, especially a temperature -sensitive polymer molecule brush and preparation methods based on elastic protein peptides. Background technique [0002] As a naturally existed peptide, elastin-like polypeptides (ELPS) exists in the skin, heart, lungs and other tissues and organs of the organisms.The use of elastic protein peptide to prepare synthetic intelligent materials has also become the focus of research.The elastic protein peptide in the organism has a variety of different amino acid sequences, and its universal sequence structure is (XPGXG) n (P is proline proline, pro; g is glyine, gly; x is any amino acid except proseine).In fact, the most content is (VPGVG) n (V is Valine, Val) sequence, due to its good biocompatibility, it has important application value in the field of biomaterials.Elastic protein peptides have temperature sensitive be...

AI Technical Summary

Problems solved by technology

At present, there are many researches based on elastin peptides, mainly in the field of biology. The temperature-sensitive linear ELPs containing hundreds or even higher amino acid residues can be synthesized by using biological gene technology, but there are still some problems in these studies: (1) biological gene The technical cost is high, and the temperature-sensitive behavior of the synthesized linear structure ELPs is not sensitive enough; (2) the response behavior is single, for example, it will respond to the type and concentration of salt, but due to the small amount of pH-sensitive end groups, the response to pH is poor; ( 3) Due to the small amount of end groups, it is difficult to further functionalize

Therefore, the preparation of elastin peptide-based polymer molecular brushes has important research significance. The main problem in current research in this area is that the phase transition is not sensitive enough (N. R. Cameron, et al. Macromolecules, 2007 , 40, 6094-6099)

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