High-density distributed stereo electrode apparatus

A three-dimensional electrode, distributed technology, applied in the field of cell electroporation, can solve the problems of unsuitable high-throughput experimental operation, easy penetration of the three-dimensional electrode into tissue, low electroporation efficiency, etc., achieving low cost and making up for the electric field. The effect of unevenness and small electrode spacing

Active Publication Date: 2014-09-10
ETTA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the planar electrodes that appeared later solved the negative effects caused by excessive voltage, the amount of cells that can be processed each time is very small, which is not suitable for high-throughput experimental operations.
Three-dimensional electrodes are easy to penetrate into tissues and living bodies, and are mostly used for clinical electroporation of tumors or living tissues. The electroporation efficiency is not high, and there are no relevant reports on cell electroporation in vitro, such as suspension cells or adherent cells.

Method used

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Embodiment 1

[0026] see figure 1 , figure 2 , image 3 and Figure 4 The present invention provides a high-density distributed electrode device, including an electrode array 1 and an electrode fixing assembly, and the electrode array 1 is fixed on the electrode fixing assembly.

[0027] The electrode array 1 is composed of 37 solid cylindrical electrodes, and the arrangement rule is that all the electrodes are arranged in a regular hexagonal structure, and the distance between two adjacent electrodes is equal, and the distance between the two adjacent electrodes is equal. The spacing is the length of the sides, and the interior of the regular hexagon is divided into several small equilateral triangle units. An electrode is placed at the apex of each equilateral triangle, that is, all electrodes are divided into 3 groups of I, II, and III, and each equilateral triangle unit The vertices of belong to I, II, III respectively.

[0028] The inner diameter of the regular hexagon of the elec...

Embodiment 2

[0044] The present invention provides a high-density distributed electrode device. Other structures are the same as those in Embodiment 1. The arrangement rule of the electrode array is that all electrodes are arranged in a regular quadrilateral structure, and the distances between two adjacent electrodes are equal, so that The distance between two adjacent electrodes is the side length. Divide the interior of the regular quadrilateral into several small square units, and place an electrode at the vertex of each square, that is, all electrodes are divided into 4 groups of I, II, III, and IV. The vertices of the four square units belong to I, II, III, and IV respectively, and the electrodes of one group are used as positive electrodes, and the electrodes of the other groups are used as negative electrodes, and electric pulses are applied in turn.

Embodiment 3

[0046] The present invention provides a high-density distributed electrode device. Other structures are the same as those in Embodiment 1. The arrangement rule of the electrode array is that all electrodes are arranged in a regular hexagonal structure, and the distance between two adjacent electrodes is equal. , taking the distance between two adjacent electrodes as the side length, divide the plane into several small regular hexagonal units, and place an electrode at the vertex of each regular hexagonal unit, that is, all electrodes are divided into I, II, III, There are 6 groups of IV, V, and VI. The vertices of each regular hexagonal unit belong to I, II, III, IV, V, and VI respectively. The electrodes of one group are used as positive electrodes, and the electrodes of the remaining groups are used as negative electrodes. Electric pulses are applied in turn. .

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Abstract

The invention discloses a high-density distributed three-dimensional electrode device, which includes an electrode array and an electrode fixing assembly. The electrode array is fixed on the electrode fixing assembly. The electrode array includes a plurality of electrodes. An electric pulse of the first polarity and an electric pulse of the second polarity are applied, wherein the electrodes corresponding to the electric pulse of the first polarity are distributed around the electrodes corresponding to the electric pulse of the second polarity. Through the above method, the present invention provides a high-density distributed three-dimensional electrode device. The device uses group-multiplexed electrodes, which can compensate for the uneven electric field caused by the three-dimensional electrodes to the greatest extent, and can process milliliter-level cells at a time. It can be used in both orifice plates and flow devices, can avoid damage to cells by high voltage, and is low in cost. It is a high-throughput, high-efficiency cell electroporation device.

Description

technical field [0001] The invention relates to cell electroporation technology, in particular to a high-density distributed three-dimensional electrode device. Background technique [0002] Electroporation technology has been used to insert molecules into animal cells or plant cells since the 1970s. Researchers have confirmed that exposure of cells to short-term and continuous high-voltage electric fields can form channels on the cell membrane, and macromolecules such as proteins and DNA can pass through. This channel enters the cell. These channels, known as electropores, are regions of increased permeability caused by local disruption of the cell membrane caused by high-voltage electric fields. These holes are short-lived but sufficient for the entry of large molecules such as plasmid DNA molecules into the cell. Cells can tolerate the formation of these holes, but if too many and large holes are formed, the process by which they are created, and the molecules introduce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
CPCC12M1/42C12M35/02C12N15/87
Inventor 李志宏梁子才吴梦希闫浩赵德尧
Owner ETTA BIOTECH
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