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A kind of culture medium and fermentation method for fermenting and producing acetylisovaleryl tylosin by Streptomyces thermotolerant

A technology of acetylisovaleryl Tylos and heat-resistant Streptomyces, which is applied in the field of fermentation, can solve the problems of high production cost, unsuitability for large-scale industrial production, and easy pollution of the environment, and achieve the effect of shortening the production cycle and improving production efficiency

Active Publication Date: 2015-09-30
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 Synthetic method is used to produce acetylisovaleryl tylosin. The organic solvent used in the production process is highly toxic, easy to pollute the environment, and the production cost is relatively high. Not suitable for industrialized mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Seed culture medium preparation process: at 1.5m 3 Add 20kg of corn starch, 25kg of soybean meal, 15kg of yeast powder, 30L of corn syrup, 6kg of ammonium sulfate, 2kg of potassium dihydrogen phosphate, 3kg of magnesium sulfate, and 0.1L of organic silicon to the seed tank. The volume of seed liquid is 1m 3 .

[0064] Fermentation medium preparation process: at 15m 3 Add 100 kg of soybean oil, 200 kg of corn starch, 50 kg of low-temperature soybean meal powder, 200 kg of formula fish meal, 150 kg of peanut meal powder, 80 kg of yeast powder, 30 kg of ammonium sulfate, 30 kg of potassium dihydrogen phosphate, 40 kg of magnesium sulfate, 0.1 kg of zinc sulfate, and chlorine into the fermentation tank. 0.6kg of iron, 0.01kg of cobalt chloride; 1L of polyether modified silicon, the volume of fermentation broth is 10m 3 . The formula fish meal is prepared according to 140kg fish meal, 20kg bone meal and 40kg soybean meal powder.

[0065] The seed culture medium is sterilized,...

Embodiment 2

[0069] Seed culture medium preparation process: at 1.5m 3 Add 22kg of wheat starch, 28kg of soybean powder, 17kg of yeast powder, 35L of corn steep liquor, 7kg of ammonium nitrate, 2.5kg of potassium dihydrogen phosphate, 4kg of magnesium sulfate, and 0.15L of organic silicon into the seed tank. The volume of seed liquid is 1m 3 .

[0070] Fermentation medium preparation process: at 15m 3 Add 120kg of corn oil, 250kg of wheat starch, 75kg of low temperature soybean meal powder, 250kg of formula fish meal, 180kg of peanut meal powder, 90kg of yeast powder, 35kg of ammonium nitrate, 35kg of potassium dihydrogen phosphate; 50kg of magnesium sulfate, 0.2kg of zinc sulfate, Ferric chloride 0.7kg, cobalt chloride 0.02kg; polyether modified silicon 1.5L, fermentation broth volume is 10m 3 . The formula fish meal is prepared according to 180kg of fish meal, 25kg of bone meal and 45kg of soybean meal.

[0071] The seed culture medium is sterilized, cooled, and kept under pressure with s...

Embodiment 3

[0075] Seed culture medium preparation process: at 1.5m 3 Add 25kg of sweet potato starch, 30kg of soybean powder, 20kg of yeast powder, 40L of corn steep liquor, 8kg of ammonium sulfate, 3kg of potassium dihydrogen phosphate, 5kg of magnesium sulfate, and 0.2L of organic silicon to the seed pot. The volume of seed liquid is 1m 3 .

[0076] Fermentation medium preparation process: at 15m 3 Add 150kg of rapeseed oil, 300kg of sweet potato starch, 100kg of low-temperature soybean meal powder, 300kg of formula fish meal, 200kg of peanut meal powder, 100kg of yeast powder, 40kg of ammonium nitrate, 40kg of potassium dihydrogen phosphate; 60kg of magnesium sulfate and 0.3kg of zinc sulfate into the fermentation tank , Ferric chloride 0.8kg, cobalt chloride 0.03kg; polyether modified silicon 2L, fermentation broth volume is 10m 3 . The formula fish meal is prepared according to 220kg fish meal, 33kg bone meal and 47kg soybean meal powder.

[0077] The seed culture medium is sterilize...

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Abstract

The invention relates to a culture medium for producing acetylisovaleryltylosin by fermenting streptomyces thermotolerans and a fermentation method. According to the culture medium and the fermentation method, a culture medium formula and a production technology are confirmed, and stable and high-efficiency production of acetylisovaleryltylosin is realized. In addition, the culture medium and the production technology can raise a fermentation unit, shorten the fermentation period, and increase the yield of acetylisovaleryltylosin.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for fermenting and producing acetylisovaleryl tylosin by Streptomyces thermosus. Background technique [0002] Acetylisovaleryltylosin (Acetylisovaleryltylosin, AIV), also known as tylosin, is a macrolide-type poultry-specific antibiotic obtained by semi-synthesis on the basis of tylosin through biological fermentation. Streptomyces thermotolerant ( Streptomyces thermotolerans ) mutant strain, which belongs to a kind of actinomycetes. As a new type of macrolide antibiotics, it overcomes the shortcomings of many macrolides, and has the characteristics of high efficiency, broad spectrum, less resistance, and less toxic side effects. It is a good antibiotic for digestive system diseases and has broad application prospects. The tartrate salt of acetylisovaleryl tylosin is common in the market, and the trade name is Ailesi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/465
Inventor 任勇王义王文超刘洋
Owner 宁夏泰瑞制药股份有限公司
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