Drum type biochip spotting device and method thereof

Abstract

The invention relates to a drum type biochip spotting device and a method thereof. The device comprises: a drum having an outer circumference surface, wherein a biochip matrix can be coated on the outer circumference surface of the drum; a drum driving mechanism used for rotating the drum; a spotting needle rack arranged over the drum and provided with a spotting needle array; and a spotting needle rack driving mechanism used for making the spotting needle rack move relative to the drum. The device uses the surface of the drum as the bearing surfaces of biochips in the invention, so the space utilization rate is substantially increased, a spotting pipeline is shortened, and the bubble generation possibility is reduced. A slide block provided with a spotting needle does not move in the vertical spotting process, so a pipeline system is always in a stationary state, and the pressure change influencing the spotting precision is very small, thereby the spotting precision and the spotting efficiency are greatly increased.

Description

technical field [0001] The invention relates to a biochip spotting device and method. Background technique [0002] In many aspects such as deciphering the mysteries of human genetics and drug screening, the biochip spotting instrument (or also called "sample spotting device") has become an indispensable basic equipment. Therefore, the level of technology and performance indicators of biochip spotters also affect the progress of life science research to some extent. As far as the function is concerned, no matter what kind of biochip spotter, it is to be able to spot the DNA, RNA, polypeptide, protein and other biological components to be researched or tested in the desired matrix with high density and high precision. Immobilized on solid-phase dense carriers such as silicon wafers, glass wafers, or pottery wafers in a manner to form biomolecular lattices. [0003] As the support and executor of precision, speed, function, etc., the mechanical structure plays a pivotal role...

AI Technical Summary

Problems solved by technology

Such a mechanical structure layout is relatively complicated and loose, and the movement range of the slide table carrying the biochip (substrate) is large, and the space utilization rate is not high.

The spotting instrument of this structure occupies a large area in use, and because the spotting pipeline of the spotting needle (in the existing spotting instrument, the lengthy length of bending is usually used between the spotting needle and the sample container). The pipeline is connected, which can be referred to as "sampling pipeline" for short) is very long, and the possibility of air bubbles is relatively high during sample application

In addition, during the sampling process, the sampling needle needs to move continuously, the pipeline system is also unstable, and the pressure change that affects the sampling accuracy is relatively large; this in turn leads to low sampling efficiency, low sampling accuracy, and biochip processing The time is relatively long, which makes it difficult for most scientific research institutions to accept

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