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Method for improving viscosity of gelatin by using glutamine transaminase for catalyzing gelatin crosslinking

A transaminase catalyzed gelatin and glutamine technology, which is applied in the fields of food and medicine, can solve the problems of limiting the application of gelatin and the toxicity of cross-linking agents, and achieve the effects of cheap and easy-to-obtain raw materials, low production costs, and safe methods

Inactive Publication Date: 2013-09-11
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious defects in the traditional chemical cross-linking method, because most of the cross-linking agents are toxic, which limits the application of cross-linked gelatin in the fields of food and medicine

Method used

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  • Method for improving viscosity of gelatin by using glutamine transaminase for catalyzing gelatin crosslinking

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Place 5 parts by weight of low-viscosity alkali-process bone gelatin in a reactor, add water to swell for 40 minutes, and stir at 50°C to dissolve the bone gelatin in the reactor to obtain a bone gelatin solution with a mass concentration of 5%. ;

[0024] (2) the pH value of the bone gelatin solution obtained by adjusting step (1) with aqueous sodium hydroxide solution is 7; according to the total amount of bone gelatin, add microbial transglutaminase, wherein the mass ratio of microbial transglutaminase to bone gelatin 1:2000; Stir at a temperature of 60°C for 6 hours to obtain a reaction solution;

[0025] (3) The temperature of the reaction solution obtained in step (2) was raised to 95° C., stirred for 15 minutes to inactivate the enzyme, and bone gelatin glue was obtained. The bone gelatin glue can be further freeze-dried or triple-effect evaporated, concentrated and then dried with a fourdrinier net to obtain a bone gelatin product. The product indexes of t...

Embodiment 2

[0027] (1) Put 10 parts by weight of low-viscosity alkali-process bone gelatin in a reaction kettle, add water to swell for 40 minutes, and stir at 50°C to dissolve the bone gelatin in the reaction kettle to obtain a bone gelatin solution with a mass concentration of 10%. ;

[0028] (2) the pH value of the bone gelatin solution that step (1) obtains with hydrochloric acid regulation is 5; According to the total amount of bone gelatin, add microorganism transglutaminase, wherein the mass ratio of microorganism transglutaminase and bone gelatin is 1: 1000; stirring at a constant temperature for 3 hours at a temperature of 37° C. to obtain a reaction solution;

[0029] (3) The temperature of the reaction solution obtained in step (2) was raised to 95° C., stirred for 15 minutes to inactivate the enzyme, and bone gelatin glue was obtained. The bone gelatin glue can be further freeze-dried or triple-effect evaporated, concentrated and then dried with a fourdrinier net to obtain a ...

Embodiment 3

[0031] (1) Put 10 parts by weight of low-viscosity alkali-process bone gelatin in a reaction kettle, add water to swell for 40 minutes, and stir at 50°C to dissolve the bone gelatin in the reaction kettle to obtain a bone gelatin solution with a mass concentration of 10%. ;

[0032] (2) the pH value of the bone gelatin solution that step (1) obtains with phosphoric acid adjustment is 5; According to the total amount of bone gelatin, add microorganism transglutaminase, wherein the mass ratio of microorganism transglutaminase and bone gelatin is 1: 4000; stirring at a constant temperature for 10 hours at a temperature of 50°C to obtain a reaction solution;

[0033] (3) The temperature of the reaction solution obtained in step (2) was raised to 100° C., stirred for 15 minutes to inactivate the enzyme, and the bone gelatin glue was obtained. The bone gelatin glue can be further freeze-dried or triple-effect evaporated, concentrated and then dried with a fourdrinier net to obtain ...

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Abstract

The invention belongs to the field of food and medicine and relates to a method for improving the viscosity of gelatin by using a glutamine transaminase for catalyzing gelatin crosslinking. According to the method, gelatin molecules are catalyzed to crosslink by using the glutamine transaminase at a certain pH value and temperature, the adjustment on the viscosity of the gelatin can be realized through controlling the reaction time and the addition of the glutamine transaminase. The method can be widely applied to bone gelatin and skin gelatin which are prepared by acid, alkali and enzyme methods. The method disclosed by the invention is safe and non-toxic, is simple in process and is applicable to gelatin production plants or application units; and the viscosity of the gelatin is improved through the method disclosed by the invention, so that the quality and application value of the gelatin are improved.

Description

technical field [0001] The invention belongs to the field of food and medicine, and relates to a method for cross-linking gelatin catalyzed by transglutaminase, specifically a method for increasing the viscosity of gelatin by catalyzing the cross-linking of gelatin molecules by transglutaminase under certain temperature and pH conditions . Background technique [0002] Gelatin is made from the bones and skins of cattle, pigs and other animals. After a series of pretreatments and purifications, the collagen is extracted, degraded appropriately, and then thermally denatured. According to different raw materials, gelatin can be divided into bone gelatin and skin gelatin, and according to different production processes, it can be divided into acid-process gelatin and alkali-process gelatin. [0003] Gelatin has high nutritional value, it contains 18 kinds of amino acids, they are alanine, glutamic acid, threonine, glycine, histidine, leucine, lysine, methionine, proline, serine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00
Inventor 王毅虎张兵郭燕川王颖史京京
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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