Method for preparing compound enzyme and probiotic preparation for feed through biotransformation of broad bean stems/leaves and cottonseed meals

A technology of probiotic preparations, broad bean stems and leaves, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effects of low-cost and high-efficiency biotransformation, high enzyme activity, and simple equipment structure

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broad bean stems and leaves, 5kg of cottonseed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of the starter in step 1) with 600kg of broad bean stems and leaves, 300kg of cottonseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 28.55IU / g; total cellulase activity reaches 3.32IU / g; beta-glucosidase activity reaches 3.32IU / g; reached 7.89IU / g; endoglucana...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broad bean stems and leaves, 5kg of cottonseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of the starter in step 1) with 600kg of broad bean stems and leaves, 300kg of cottonseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 28.24IU / g; total cellulase activity reaches 3.18IU / g; beta-glucosidase activity reaches 3.18IU / g; reached 7.38IU / g; endoglucana...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broad bean stems and leaves, 5kg of cottonseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of broad bean stems and leaves, 300kg of cottonseed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 29.28IU / g; total cellulase activity reaches 3.18IU / g; beta-glucosidase activity reaches 3.18IU / g; reached 7.55IU / g; endo...

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PUM

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed through the biotransformation of broad bean stems / leaves and cottonseed meals. The method comprises the steps of: thoroughly mixing cooked Pu'er tea, mushroom sticks, tea tree mushroom sticks, first broad bean stems / leaves, first cottonseed meals, first wheat bran, first apple pomace and first water which are subjected to pile fermentation, and carrying out acclimation culture so as to form a leavening agent; and thoroughly mixing the leavening agent, second broad bean stems / leaves, second cottonseed meals, second wheat bran, second apple pomace and second water, and carrying out fermentation, thereby obtaining the compound enzyme and probiotic preparation for feed. According to the method, the compound enzyme and probiotic preparation with better various performance indexes is prepared through fermenting the broad bean stems / leaves and the cottonseed meals by using food-grade safe flora with stable microbial states, so that the low-cost and high-efficiency biotransformation of the broad bean stems / leaves and the cottonseed meals is realized. Meanwhile, the method disclosed by the invention has the advantages that microbes grow easily, the enzyme activity is high, the fermentation process is extensive, and the industrial large-scale production is facilitated.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting broad bean stems and leaves and cottonseed cake by adopting microecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖吴渊伍星主纪晓燚于骅诸文颖陈功杜姗姗杨璐
Owner ZHEJIANG UNIV
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