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Affinity adsorption material for treating hyperbilirubinemia and preparation method thereof

A technology for hyperbilirubinemia and adsorption materials, applied in the field of bioseparation engineering, can solve the problems of difficult clinical application of materials, complicated preparation methods of cross-linked macroporous chitosan microspheres, etc. Mild conditions and efficacy in the treatment of hyperbilirubinemia

Inactive Publication Date: 2015-06-17
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption capacity of this kind of material to bilirubin is 2.56-4.66mmol / g, but due to the complicated preparation method of cross-linked macroporous chitosan microspheres, it is difficult to apply this kind of material in clinical practice
[0005] At present, there is no information about the synthesis of poly(styrene-acrylic acid) cross-linked polymer microspheres by suspension polymerization as a carrier to immobilize human serum albumin that can specifically bind to bilirubin, and to prepare affinity adsorbents for Related reports on clearing bilirubin in blood

Method used

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  • Affinity adsorption material for treating hyperbilirubinemia and preparation method thereof
  • Affinity adsorption material for treating hyperbilirubinemia and preparation method thereof
  • Affinity adsorption material for treating hyperbilirubinemia and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Step 1: Mix 1.5g AA and 10.5g St, add 0.96g DVB, 0.12g BPO in sequence, and stir well;

[0024] Step 2: Add the mixed solution in step 1 to 48 g of deionized water containing 0.5 g of PVA, stir mechanically at 600 rpm, react at 75°C for 4 hours, and cure at 90°C for 2 hours to obtain poly(styrene-acrylic acid) microspheres;

[0025] Step 3: Disperse the poly(styrene-acrylic acid) microspheres in step 2 in deionized water, adjust the concentration to 100g / L, and add 2.95g of NHS;

[0026] Step 4: Add 4.61 g of EDC in Step 3 and stir at room temperature for 8 h. The obtained product was repeatedly washed with deionized water, suction filtered, and vacuum-dried to constant weight to obtain activated poly(styrene-acrylic acid) microspheres;

[0027] Step 5: Disperse the activated poly(styrene-acrylic acid) microspheres in step 4 in phosphate buffer solution (pH=7.4), and adjust the concentration to 100g / L. Add 2.4g HAS and stir at room temperature for 12h. The obtained p...

Embodiment 2～7

[0029] The steps are the same as in Example 1, changing the amount of raw materials, cross-linking agent, initiator, stabilizer, dehydrating agent, catalyst, solvent, changing the conditions such as reaction temperature, reaction time, mechanical stirring speed, etc., to obtain human serum albumin-immobilized poly (Styrene-acrylic acid) microspheres, see Table 1 for steps 1 and 2, and see Table 2 for steps 3 and 5.

[0030] Table 1

[0031]

[0032] Table 2

[0033]

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PUM

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Abstract

The invention provides a preparation method of an affinity adsorption material for treating hyperbilirubinemia. An affinity adsorber is prepared by taking poly(styrene-acrylic acid) micro-spheres which are synthesized by a suspension polymerization method as carriers and fixing human serum albumin by chemical bonds. The human serum albumin can be specifically bonded with bilirubin in blood without influence on normal constitution of the blood. The preparation method provided by the invention is simple in preparation process and moderate in reaction conditions; the prepared affinity adsorber is regular in morphology and has a strong specific bonding effect on the bilirubin in the blood. The clearance rate of the affinity adsorber for the bilirubin in the blood is 44.3%-62.7%; therefore, the affinity adsorber can be used for the field of blood purification and applied to treating the hyperbilirubinemia.

Description

technical field [0001] The invention relates to the technical field of bioseparation engineering, in particular to the preparation of an affinity adsorption material specifically binding to bilirubin for treating hyperbilirubinemia. Background technique [0002] Bilirubin is a lipophilic toxin that is a breakdown product of hemoglobin in human aging red blood cells. Under normal circumstances, it is combined with glucuronic acid by the liver, and then excreted after further metabolism. If the liver function is disturbed, the concentration of bilirubin in the blood will increase. When the blood bilirubin concentration exceeds 17mmol / L, hyperbilirubinemia symptoms such as light yellow to deep golden yellow skin and sclera, skin itching, indigestion, abdominal pain and fever will appear; and it can pass through the blood-brain barrier Combined with nerve nuclei, it interferes with the normal metabolism and function of brain cells, and produces irreversible toxic side effects ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61K47/48A61P1/16C08F212/08C08F220/06C08F2/20C08F8/00
Inventor 戴李宗宋存峰常迎余世荣毛杰
Owner XIAMEN UNIV
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