Microbial agent and preparation method as well as application thereof

A technology for microbial inoculants and seeds, applied to microbial inoculants and their preparation methods and application fields, can solve the problems of high requirements for composting equipment, incomplete fiber degradation, long composting time, etc., and achieve good control effect and bacterial species composition structure. Simple, fast fermentation effect

Active Publication Date: 2013-09-18
南京宁粮生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The cellulose in crop straws mostly forms dense crystallization regions, and is embedded in hemicellulose and lignin at the same time, making it difficult to enzymatically decompose. Therefore, the natural composting process of straws has long composting time, large nutrient loss, and incomplete composting of fiber degradation. There are many problems such as poor quality. At present, there are many kinds of bacterial agents used to accelerate the composting process and improve the composting effect at home and abroad. The bacterial agent used for composting cellulosic waste has the characteristics of a large number of bacterial species, strong specificity of fermented materials and high requirements for composting equipment, resulting in high composting costs. The patent with publication number CN101486597A provides a composite fermentation bacterial agent , the bacteria agent was added to the bacteria agent 1# and the bacteria agent 2# in the early and late stages of composting, which not only realized the rapid decomposition of the cellulose material, b

Method used

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  • Microbial agent and preparation method as well as application thereof
  • Microbial agent and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1 Preparation of bacterial agent

[0026] 1) Fermentation culture of Bacillus subtilis and Bacillus megaterium

[0027] LB medium: yeast powder 5 g / L, peptone 10 g / L, NaCl 10 g / L, pH 7.0, sterilized at 121 °C for 25 min.

[0028] Fermentation medium involved in seed tank and fermenter: glucose 5 g / L, peptone 10 g / L, NaCl 10 g / L, pH 7.0, sterilized in real tank at 121 °C for 25 min.

[0029] The slanted strains stored at 4°C were inoculated into a 250 mL shake flask containing 100 mL of LB medium, activated and cultured at 35°C at 170 r / min for 24 h, and then the activated bacterial solution was added at a 10% (v / v) concentration. The inoculum was transferred into a 1000 mL shake flask containing 500 mL of LB medium, and cultured at 35 °C for 12 h at 170 r / min to obtain seed liquid.

[0030] The above-mentioned seed liquid was inserted into the fermentation medium in the seed tank according to the inoculum amount of 10% (v / v), fermented and cultured at 35 ° C a...

Example Embodiment

[0040] Example 2 Preparation of inoculum

[0041] The preparation of bacterial and fungal fermentation bacteria liquid is identical with 1 and 2 in Example 1, and the fermentation bacteria liquid of Bacillus subtilis, Bacillus megaterium, Trichoderma longbranchus and Aspergillus niger is 1: 1: 1: 1 by mass ratio Mix well, then adsorb on vermiculite at a mass ratio of 1:5, then dry at 35~40°C until the water content is less than 15%, and test it according to the standard of agricultural microbial inoculum (GB 20287-2006). Obtain the bacterial agent.

Example Embodiment

[0042] Example 3 Experiment on composting wheat grass with microbial inoculants

[0043] The wheat straw was crushed into straw segments of 10-15 cm, and the microbial inoculum prepared in Example 1 was added according to the mass ratio of 2%. The soil is mixed, and then the mixture and straw are evenly mixed, and the water content of the pile is adjusted to about 55~65%. When the temperature rises to 60°C, the piles are turned to supply oxygen in sequence, and the piles are turned once every 8 days, for a total of two times. The body volume is reduced to about 1 / 2 of the original, that is, high-efficiency wheat straw organic fertilizer is obtained.

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Abstract

The invention relates to a microbial agent and a preparation method as well as an application of the microbial agent. The microbial agent comprises functional bacteria and an adsorbent and is characterized in that the functional bacteria comprise bacillus subtilis, bacillus megatherium, trichoderma longibrachiatum and aspergillus niger, wherein the mass ratio of the microbial fermentations of the bacillus subtilis, the bacillus megatherium, the trichoderma longibrachiatum and the aspergillus niger in a mixture is 0.5-2:0.5-2:0.5-2:0.5-2. The microbial agent is prepared by carrying out the processes of slant culture, shake-flask culture, seeding tank fermentation, fermentation tank fermentation, microbial fermentation mixing, absorbent absorption, and low-temperature drying, and the microbial agent can be applied to the production of straw manure.

Description

technical field [0001] The invention relates to a microbial agent and a preparation method and application thereof, belonging to the field of biotechnology, in particular to a microbial agent capable of promoting rapid composting of crop stalks such as wheat straw, rice straw and corn stalks and a preparation method thereof. technical background [0002] my country is a large agricultural country and one of the countries with the most abundant crop straw in the world. The annual straw production is about 700 million tons, and with the increase of crop yield, the straw production will further increase. A large amount of straw is discarded or burned, which is not only a waste of resources, but also brings huge environmental pollution and ecological damage, seriously endangering people's health. [0003] On the other hand, my country has a large population and a shortage of cultivated land resources, and the multi-cropping index of farmland is high. The resulting depletion of so...

Claims

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Application Information

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IPC IPC(8): C05F17/00
CPCY02W30/40
Inventor 梁晓辉何建桥常志州黄红英徐茂管永祥陈光亚王绪奎殷广德梁永红吴昊孔国顺马宏卫戴波吴息正梅成兵邵孝侯俞禄窦培冲赵敏
Owner 南京宁粮生物工程有限公司
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