Purification technology of phycocyanin

A technology for phycocyanin and fresh algae, which is applied to the biological field, can solve the problems of high cost, low purity and low phycocyanin purity yield, and achieves simple method, improved economic benefit, high purification efficiency and high concentration efficiency. Effect

Inactive Publication Date: 2013-09-18
DAFENG CIBAINIAN BIOTECH
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Problems solved by technology

[0002] Since the 21st century, the role of phycocyanin has attracted much attention, especially a lot of research on the extraction and purification of phycocyanin, but the purity of phycocyanin in known technologies is not high
Japanese DIC is the method published in the world and some literatures, which are completely limited to the laboratory scale, including salting out, gel filtration, adsorption chromatography, ion exchange chromatography, and other more expensive methods, most of which are not suitable for the preparation of food Pigment Grade Products
These methods are costly first, and secondly, the purity obtained is not high

Method used

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Embodiment Construction

[0010] 1. Extract the crude extract. Take fresh spirulina algae, add an equal volume of deionized water, mix well, and then freeze at -20°C to -10°C. After freezing, take it out and let it thaw at room temperature, and freeze and thaw for 3-5 times. Most of the phycocyanin is extracted from the cells.

[0011] 2. Then use 120-mesh gauze to filter to obtain the filtrate and carry out centrifugation to separate the crude extract from other impurities, and the obtained phycocyanin has a purity of 0.6 or more.

[0012] 3. Two-phase extraction-ultrafiltration separation and purification technology, through two-phase extraction, further separate phycocyanin and other proteins, thereby improving the purity of phycocyanin, and then use ultrafiltration technology to obtain phycocyanin with high purity Protein ≥ 4.0, the purity increased to more than 600 times the original.

[0013] 4. Freeze and dry naturally at a low temperature of 5°C, with a moisture content of ≤7.0%. So far, the ...

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Abstract

The invention provides a preparation method of phycocyanin and belongs to the technical field of organisms. The preparation method comprises the following steps of: firstly, taking fresh algae or spirulina powder as a material; firstly, extracting coarse filtrate of phycocyanin, adding deionized water after dewatering the material; evenly mixing, and freezing at -20 DEG C to -10 DEG C; taking out and thawing at 35 DEG C after freezing; refreezing and thawing for 3-4 times, and filtering by a 120-mesh gauze; secondly, centrifugally separating to obtain supernate, carrying out ultra-filtration separation purification after carrying out dual aqueous phase extraction on the supernate; finally freezing and drying, wherein the concentration of the obtained phycocyanin can be up to over 4.0, and finally storing the phycocyanin at 3-5 DEG C. The method disclosed by the invention is simple and convenient, and high in purification efficiency when the cost is reduced, and has good application, social and economic benefits; and the purity is over 4.0.

Description

technical field [0001] The present invention relates to the field of biotechnology. Background technique [0002] Since the 21st century, the role of phycocyanin has attracted much attention, especially a lot of research on the extraction and purification of phycocyanin, but the purity of phycocyanin in known technologies is not high. Japanese DIC is the method published in the world and some literatures, which are completely limited to the laboratory scale, including salting out, gel filtration, adsorption chromatography, ion exchange chromatography, and other more expensive methods, most of which are not suitable for the preparation of food Pigment grade product. These methods cost high at first, and the purity that the second obtains is not high. With the in-depth research and further popularization of phycocyanin fluorescent reagents, in order to realize the application of phycocyanin in biochemical fields such as fluorescent probes, it is necessary to achieve relative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/36C07K1/34C07K1/14
Inventor 汪世军
Owner DAFENG CIBAINIAN BIOTECH
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