Trypsinogen-2 detection kit and preparation method

A trypsinogen and detection kit technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as long time consumption, cumbersome detection process, and inability to quantitatively detect, and achieves guaranteed stability, good specificity, and guaranteed sensitivity. Effect
CN103308681BActive Publication Date: 2015-07-29WUHAN LIFE ORIGIN BIOTECH LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN LIFE ORIGIN BIOTECH LTD
Publication Date
2015-07-29

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Abstract

The invention discloses a trypsinogen-2 content detection kit and a preparation method thereof. The detection kit consists of reagent I and reagent II which are independent of each other, wherein the reagent I comprises the following components: a biological buffer, a preservative, inorganic salt and water; the reagent II comprises the following components: latex granules coated by trypsinogen-2 antibody, the biological buffer, the preservative and water. The trypsinogen-2 content detection kit adopts the latex granules with the particle size of 100-300nm to prepare the latex granules coated by the trypsinogen-2 antibody, thus effectively guaranteeing the sensitivity of the kit, the detection linearity range, the measurement value accuracy and the bottle opening stability; furthermore, the latex granules coated by the antibody are prepared by a chemical cross-linking method, so that the combination stability between the antibody and the latex granules can be guaranteed, and the stability of the detection kit is improved. The detection kit has the advantages of being good in stability, high in sensitivity, high in specificity, simple and convenient to operate, etc.
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Description

technical field

[0001] The present invention relates to a test kit for detecting trypsinogen-2 content, in particular to a test kit for detecting trypsinogen-2 content in the human body and a preparation method thereof. The method for using the content of 2 belongs to the detection field of trypsinogen-2 content in human body. Background technique

[0002] Acute pancreatitis (AP) is one of the common acute abdomens in clinical practice. According to foreign statistics, its incidence is 6.5-80 / 100,000 people, accounting for about 10% of acute abdomens. Some scholars believe that its pathogenesis is related to the excessive activation of trypsin caused by genetic changes. The onset of this disease is acute and the condition is dangerous. About 20% to 30% of AP cases can be transformed into necrotizing pancreatitis, leading to severe complications such as sepsis. Therefore, early diagnosis and treatment are very important. Blood amylase (AMS) is a commonly used detection item...

Claims

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