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Separation and purification method of steviol glycosides in crude stevia rebaudiana

A technology for separation and purification of steviol glycosides, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as low recovery rate, limited application, and difficulty in achieving separation effects, and achieve high-efficiency separation effects

Inactive Publication Date: 2016-06-29
苏州迪科传动机械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since different MARs have different structural parameters such as polarity, functional group, pore size, pore volume, and specific surface area, and a certain MAR has different degrees of adsorption on multiple components, therefore, for some substances, the structure is relatively similar. For the separation of components, it is often difficult to achieve a good separation effect by using a single type of MAR. Usually, the recovery rate of the product with high purity after separation is low, and the product with high recovery rate is low in purity, which limits the application of MAR to a certain extent. develop well
[0007] Using static adsorption, although multiple experiments can be carried out in parallel under the same conditions, at the same time, adsorption can be carried out under isothermal conditions, and the relationship between the adsorption amount and time or equilibrium concentration can be studied, and the adsorption kinetic curve or adsorption isotherm can be obtained for correlation. theoretical research, but it is not suitable for large-scale industrial production, which limits the application of MAR in industrial production

Method used

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  • Separation and purification method of steviol glycosides in crude stevia rebaudiana

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Experimental program
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Effect test

Embodiment 1

[0055] Such as figure 1 As shown, it is a process flow diagram of the method for separating and purifying steviol glycosides in the stevioside crude product of the present invention, and the specific steps include:

[0056] Put 10.0g of MAR into a 100mL beaker, and perform activation and purification as described above;

[0057] Put the above-treated MAR into an exchange column with an inner diameter of 1.6cm, and prepare a 0.05-0.6g / L solution of stevia crude product as an adsorption liquid, and make it flow through a macroporous resin resin mixed bed at a speed of 1BV / h , for adsorption;

[0058] After the adsorption is finished, collect the adsorption raffinate, measure the content of stevioside in the raffinate by HPLC, and calculate the adsorption rate and adsorption amount according to formula (1)-(2);

[0059] Wash the above-mentioned MAR column with distilled water 3 times, each interval is 5min, then control the flow rate to 3BV / h, add 5%, 60% ethanol and a mixture ...

Embodiment 2 and 3

[0062] Below in conjunction with embodiment 2 and 3, the present invention will be further described:

Embodiment 2

[0064] Weigh the MAR mixed bed AB-8+LSA-21 with a dry weight of about 10.0g, put it into a 100mL beaker, first add a certain volume of ethyl acetate to soak for 24 hours, then soak the liquid everywhere, then add a certain volume of ethanol to soak for 24 hours, Recover soaking solution. Then add an appropriate amount of ethanol to wash until 3 times the volume of distilled water is added and no white turbidity appears. Add an appropriate amount of distilled water to wash the resin, soak for 5 minutes and discard the washing solution, repeat this three times, so that the ethanol remaining in the resin can be gradually replaced by water more fully. Put the activated AB-8 into an exchange column with an inner diameter of 1.6cm, accurately pipette 130mL of the crude stevioside solution with a total concentration of 7.00mg / mL, control the flow rate to 1BV / h for adsorption, and receive the raffinate. The content of stevioside in the raffinate was determined by HPLC. Wash the adso...

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Abstract

A method for separating and purifying stevioside in crude stevia rebaudiana comprises the following steps: 1, filling an activated macroporous adsorption resin into an exchange column by adopting a wet process; 2, processing the crude stevia rebaudiana to prepare a crude stevia rebaudiana solution having a concentration of 0.05-0.6g / L and used as an adsorbing liquid, and allowing the crude stevia rebaudiana solution to flow through a macroporous resin mixed bed for dynamic adsorption; 3, washing the adsorbed macroporous resin mixed resin obtained in step 2 with distilled water, adding eluants comprising 5% ethanol, 60% ethanol and a mixed liquid comprising ethanol and ethyl acetate, and allowing the eluants to flow through the resin mixed bed at a same flow velocity for gradient elution; and 4, carrying evaporating concentration of the obtained stripping liquid, and drying to obtain stevioside.

Description

technical field [0001] The invention relates to a method for separation and purification of natural sweeteners with high sweetness and low calorific value, in particular to a method for adsorption separation and purification of stevioside. Background technique [0002] Stevia rebaudiana (stevia rebaudiana), also known as stevia ﹑ sugar grass, belongs to Compositae (Comppositae), Stevia genus, and its origin is in the subtropical region of South America. Since 1976, scientific research units such as Nanjing Zhongshan Botanical Garden and Chinese Academy of Agricultural Sciences have successively introduced stevia from Japan for trial planting. Stevia contains 14 kinds of trace elements and 32 kinds of nutrients. It is not metabolized, not accumulated, not carcinogenic, and non-toxic in the body. Its safety has been recognized by the international FAO and WHO and the US FAD. Sugar source, but also a good source of nutrition. [0003] Steviol glycosides, referred to as stevio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256C07H1/08
Inventor 陈振斌龙佳朋刘晓娇
Owner 苏州迪科传动机械有限公司