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Vibrio anguillarum 01 serotype inactivated vaccine, preparation method and use method thereof

An inactivated vaccine, Vibrio eel technology, applied in antibacterial drugs, pharmaceutical formulations, medical preparations containing active ingredients, etc. The problem of frequent occurrence of diseases in farmed animals can improve the level of specific immune response, avoid economic losses and enhance immunity.

Active Publication Date: 2013-10-02
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the intensive and rapid development of the aquaculture industry for many years, coupled with the continuous deterioration of the aquaculture environment and the decline in the stress resistance of the aquaculture species, it eventually led to frequent occurrence of marine animal diseases, resulting in direct economic losses of 13 billion to 15 billion yuan per year, which has become a restricted aquaculture. The bottleneck of the sustainable and healthy development of the industry

Method used

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  • Vibrio anguillarum 01 serotype inactivated vaccine, preparation method and use method thereof
  • Vibrio anguillarum 01 serotype inactivated vaccine, preparation method and use method thereof
  • Vibrio anguillarum 01 serotype inactivated vaccine, preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Pathogenicity of Vibrio anguillarum CGMCC No.6942 to flounder (Paralichthys olivaceus)

[0025] 1) Inject the median lethal dose (LD) of infected flounder 50 )

[0026] Healthy test fish with a body weight of 10-12g are temporarily raised in a 1000L aquarium, equipped with a flowing circulating water treatment and purification system, and the temperature of the breeding water is maintained at 18-20°C. After 4 weeks of temporary breeding, they were randomly divided into 30L experimental aquariums, with 10 fish in each group, and continued to be temporarily raised for 2 weeks. In the test aquarium, the water was changed once a day in the morning and afternoon, the amount of water change was 1 / 2, and the water temperature was maintained at 18-20°C.

[0027] In the infection test, live Vibrio anguillarum CGMCC No.6942 cells were diluted with sterile PBS buffer solution to a serial concentration of 10 5 -10 9 cfu / ml bacterial suspension, each dilution was injected to inf...

Embodiment 3

[0037] Example 3: Pathogenicity of Vibrio anguillarum CGMCC No.6942 to turbot (Scophthalmus maximus)

[0038] 1) Injection of LD from infected turbot 50

[0039] Healthy test fish with a body weight of 9-12g are temporarily raised in a 1000L aquarium, equipped with a flowing circulating water treatment and purification system, and the culture water temperature is maintained at 16-18°C. After 4 weeks of temporary breeding, they were randomly divided into 30L experimental aquariums, with 10 fish in each group, and continued to be temporarily raised for 2 weeks. In the test aquarium, the water was changed once a day in the morning and afternoon, the amount of water changed was 1 / 2, and the water temperature was maintained at 16-18°C.

[0040] In the infection test, live Vibrio anguillarum CGMCC No.6942 cells were diluted with sterile PBS buffer solution to a serial concentration of 10 5 -10 9 cfu / ml bacterial suspension, each dilution was injected to infect a group of experim...

Embodiment 4

[0050] Embodiment 4: Preparation of Vibrio anguillarum inactivated vaccine

[0051] Take Vibrio anguillarum CGMCC No.6942 and inoculate it into a common broth medium containing 1.5% NaCl (w / v), and culture it in a shaker flask at 25-30°C for 24-36 hours; Concentration of 0.3-0.5% formaldehyde, placed in shake flasks at 25-30 ° C for 24-36 hours. Centrifuge the obtained culture at a speed of 4000-5000rpm / min for 10-20min, collect the precipitated bacteria, and then use PBS phosphate buffer solution to suspend the precipitate respectively, and adjust the concentration of bacterial cells to ~10 9 cells / ml, that is Vibrio anguillarum CGMCC No.6942 inactivated vaccine.

[0052] The composition of ordinary broth medium containing 1.5% NaCl (w / v) is: 10g tryptone, 5g yeast extract, 15g NaCl, 1000ml distilled water, pH7.6.

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Abstract

The invention relates to an aquaculture animal disease prevention and control technology, specifically to a Vibrio anguillarum 01 serotype inactivated vaccine, a preparation method and a use method thereof, wherein the vaccine is Vibrio anguillarum 01 serotype, the Vibrio anguillarum 01 serotype strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 7, 2012, and a preservation number is CGMCC No.6942. With the inactivated vaccine, breeding fishes can be effectively stimulated to produce specific immune response, immunity of breeding animals can be effectively enhanced, and susceptible fishes can be protected from pathogenic Vibrio anguillarum infection. In addition, the Vibrio anguillarum 01 serotype inactivated vaccine preparation method has characteristics of low raw material cost and safety on animals and environment.

Description

technical field [0001] The invention relates to the disease prevention technology of aquaculture animals, in particular to an inactivated vaccine of Vibrio anguillarum 01 serotype and its preparation and use method. Background technique [0002] my country's aquaculture output has ranked first in the world for more than ten consecutive years, and it has become one of the fastest-growing industries in large-scale agriculture. The proportion of marine aquaculture production in total aquaculture has been increasing year by year, among which marine fish aquaculture production has shown the fastest growth for more than 20 years, and has become one of the agricultural industries with the most development potential. Due to the intensive and rapid development of the aquaculture industry for many years, coupled with the continuous deterioration of the aquaculture environment and the decline in the stress resistance of the aquaculture species, it eventually led to frequent occurrence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/106A61P31/04
Inventor 莫照兰李贵阳李杰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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