Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method used for detecting expression of HER2 and MUC4 proteins and applications thereof in treatment of cancer

A protein expression and breast cancer technology, applied in the field of protein detection and nucleic acid, can solve the problem of low objective response rate, and achieve the effect of preventing the missed treatment opportunity and economic loss, and the detection results are intuitive and accurate.

Inactive Publication Date: 2013-10-02
BEIJING QINLAN BIOTECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trastuzumab is used to treat patients with metastatic breast cancer with overexpression of HER2, and has achieved good therapeutic effect. However, the objective response rate of its single treatment is not high, and the median survival period of 9 months is only 12%-34 %, and most patients develop resistance within one year of using the drug

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method used for detecting expression of HER2 and MUC4 proteins and applications thereof in treatment of cancer
  • Method used for detecting expression of HER2 and MUC4 proteins and applications thereof in treatment of cancer
  • Method used for detecting expression of HER2 and MUC4 proteins and applications thereof in treatment of cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for detecting HER2 protein expression, said method comprising the following steps:

[0041] A. Immunohistochemistry

[0042] 1) Take the pathological tissue of female patient A with breast cancer, make paraffin-embedded sections, place them in an oven at 60°C for 20 minutes, dewax the paraffin sections to water, and obtain the dewaxed sections;

[0043] 2) Place the dewaxed slices in xylene for 10 minutes, replace xylene and soak for 10 minutes, then soak in absolute ethanol for 5 minutes, 95% ethanol for 5 minutes, 80% ethanol for 5 minutes, 75% ethanol for 5 minutes, 70 Soak in % ethanol for 5 minutes, soak in PBS for 5 minutes, replace PBS and repeat soaking for 3 times, and obtain slices after preliminary treatment;

[0044] 3) Place the slices after the preliminary treatment in 3% H 2 o 2 Incubate at room temperature for 20 minutes to eliminate the activity of endogenous peroxidase, and obtain slices treated with enzyme inactivation;

[0045] 4) Rinse th...

Embodiment 2

[0074] A method for detecting HER2 protein expression, said method comprising the following steps:

[0075] A. Immunohistochemistry

[0076] 1) Paraffin-embedded sections were taken from the pathological tissue of female patient B with breast cancer, placed in an oven at 60°C for 20 minutes, and the paraffin sections were dewaxed to water to obtain the dewaxed sections;

[0077] 2) Place the dewaxed slices in xylene for 10 minutes, replace xylene and soak for 10 minutes, then soak in absolute ethanol for 5 minutes, 95% ethanol for 5 minutes, 80% ethanol for 5 minutes, 75% ethanol for 5 minutes, 70 Soak in % ethanol for 5 minutes, soak in PBS for 5 minutes, replace PBS and repeat soaking for 3 times, and obtain slices after preliminary treatment;

[0078] 3) Place the slices after the preliminary treatment in 3% H 2 o 2 Incubate at room temperature for 20 minutes to eliminate the activity of endogenous peroxidase, and obtain slices treated with enzyme inactivation;

[0079]...

Embodiment 3

[0097] A method for detecting MUC4 protein expression, said method comprising the following steps:

[0098] A. Immunohistochemistry

[0099] 1) Take the paraffin-embedded section of the pathological tissue of a female C breast cancer patient, place it in an oven at 60°C for 20 minutes, dewax the paraffin section to water, and obtain the dewaxed section; the pathological tissue of the patient The slices have passed the test, confirming that the HER2 test is positive;

[0100] 2) Place the dewaxed slices in xylene for 10 minutes, replace xylene and soak for 10 minutes, then soak in absolute ethanol for 5 minutes, 95% ethanol for 5 minutes, 80% ethanol for 5 minutes, 75% ethanol for 5 minutes, 70 Soak in % ethanol for 5 minutes, soak in PBS for 5 minutes, replace PBS and repeat soaking for 3 times, and obtain slices after preliminary treatment;

[0101] 3) Place the slices after the preliminary treatment in 3% H 2 o 2 Incubate at room temperature for 20 minutes to eliminate t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method used for detecting expression of HER2 and MUC4 proteins and applications thereof in individual-based treatment of breast cancer. Pathological tissue of breast cancer patients is stained by immumohistochemical staining, so that the method is capable of detecting expression conditions of HER2 and MUC4 proteins in breast cancer tissue. The method is easy to operate, and is accurate in detecting; detection results are more visual because that a microscope is used for observation and photographing. The method is used for detecting drug resistance of the breast cancer patients against trastuzumab, so that the method is capable of providing base for diagnosis, treatment and evaluation of the breast cancer patients, and preventing the missing of treatment opportunities and economic loss which are caused by blindly applications of trastuzumab (herceptin).

Description

technical field [0001] The invention relates to nucleic acid and protein detection methods, in particular to a method for detecting HER2 and MUC4 protein expression and its application. Background technique [0002] Breast cancer is currently the highest incidence rate among women worldwide and is the leading cause of cancer death in women. With the aging of the global population and the continuous growth of the world's total population, as well as the backwardness of medical facilities in developing countries and regions, the incidence of cancer has risen sharply. According to the "Global Cancer Statistics" global tumor statistics report published online by A Cancer Journal for Clinicians in 2011, about 12.7 million cancer cases and 7.6 million cancer deaths were assessed, of which about 56% of cancer cases and 64% of Cancer deaths were from developing countries or regions. Breast cancer has a high incidence in women and is the leading cause of cancer death in women, acco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
Inventor 贾浩丛茜郭晗江丽娜路之越
Owner BEIJING QINLAN BIOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com