Cancer imaging with therapy: theranostics

A cancer and imaging technology, applied in preparations for in vivo experiments, medical preparations containing active ingredients, scientific instruments, etc., can solve the problems of undiscussed imaging and treatment combination methods, and achieve the effect of reducing side effects and less procedures

Inactive Publication Date: 2013-10-02
VIRGINIA COMMONWEALTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technique was not used in vivo, combined approaches for imaging and therapy were not discussed, and only herpesviruses and vaccinia viruses were discussed in detail

Method used

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  • Cancer imaging with therapy: theranostics
  • Cancer imaging with therapy: theranostics
  • Cancer imaging with therapy: theranostics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1. Tumor-specific imaging by gene expression driven by the progression-escalating gene-3 promoter

[0097] feed

[0098] Molecular genetic imaging is progressing from a valuable preclinical tool to guiding patient management. This strategy involves the pairing of imaging reporters with complementary imaging reagents in a system that can be used to measure gene expression, protein interactions, or track genetically marked cells in vivo. Tissue-specific promoters can be used to map gene expression in specific tissues, especially when combined with appropriate amplification mechanisms. Here we show that the progression-promoting gene-3 promoter (PEG-PROM), derived from a rodent gene that regulates the malignant phenotype, can be used to selectively drive a reporter for imaging, enabling Radionuclide-Based Molecular Imaging Techniques to Detect Micrometastatic Disease in Murine Models of Human Melanoma and Breast Cancer. Because of the robust promoter, tumor spec...

Embodiment 2

[0164] summary:

[0165] Cancer target images retain meaningful but forgettable targets. Such images can provide early diagnosis, aid in treatment planning, and are profoundly beneficial in treatment monitoring. We cross by subtraction 3 Identification of the minimal promoter region of the rodent PEG-3 gene-derived progression promotion gene-3 (PEG-Prom) 1,2 , expression of the rodent PEG-3 gene is directly associated with malignant transformation and tumor progression in rodent tumors, as well as in human tumors 3,4 , said human tumors include brain, prostate, breast, melanoma, and pancreatic 5-9 Tumor-derived cancer cell lines in . Based on these findings, we hypothesized, and subsequently demonstrated, that the systemic delivery of PEG-Prom associated with and regulated by the image construct would enable broadly applicable tumors or subtypes of different tissue origins. 10 This results in tumor-specific expression of the reporter gene not only within the primary tumor...

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Abstract

Genetic constructs comprising reporter genes operably linked to cancer specific or cancer selective promoters (such as the progression elevated gene-3 (PEG-3) promoter) are provided, as are methods for their use in cancer imaging, cancer treatment, and combined imaging and treatment protocols. Transgenic animals in which a reporter gene is linked to a cancer specific or cancer selective promoter, and which may be further genetically engineered, bred or selected to have a predisposition to develop cancer, are also provided.

Description

technical field [0001] The present invention generally relates to genetic constructs and their use in cancer imaging, cancer treatment, and methods of combining imaging and treatment regimens. Specifically, the transcription of the gene in the construct is driven by a cancer-specific promoter. Background technique [0002] Targeted imaging of cancer preserves important, but forgettable targets. Such imaging may provide early diagnosis, detection of metastases, assisted treatment planning and beneficial treatment monitoring. Molecular imaging also has the potential to generate tumor-specific reagents by balancing the expanding list of specific molecular features of tumors and their microenvironment. But many efforts at tumor-specific imaging have been accompanied by non-specific localization of putative target agents, causing unacceptably high background noise. [0003] While researchers have employed a number of strategies to deliver tumor-specific imaging agents, primari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N33/52C12N15/86C12N5/10A01K67/027C12N5/09
CPCA61K49/0013A61K51/0491C12N2710/10043G01N33/574C12Q1/6886C12Q1/6897A61K38/20A61P35/00C12Q1/04G01N33/52C12N15/86C12N5/10A01K67/027A61K49/0002A61K49/0045A61K49/04A61K51/0495C12N15/85
Inventor M·G·庞珀方孝恩P·菲舍尔
Owner VIRGINIA COMMONWEALTH UNIV
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