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Rapid propagation method for high quality seedlings of Kyara

A kind of technology of Agarwood and seedlings, which is applied in the field of rapid propagation of high-quality seedlings of Agarwood, can solve the problems of mother plant damage, the propagation of Agarwood tissue culture seedlings that have not yet been produced, and the difficulty of a large number of seeds, and achieves high application value , Simple and affordable technology

Active Publication Date: 2013-10-16
肇庆丁硕生态技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the natural state of Qinan agarwood, it is only planted every 4 years, so it is difficult to obtain a large number of seeds; at the same time, the seeds must be sown within 5 days after picking, otherwise the germination rate will be significantly reduced
However, the speed of propagation by cuttings is slow, which will cause great damage to the mother plant, and has not yet been used in large-scale production.
[0004] At present, there is no report on the tissue culture seedling propagation of Aloes chinensis at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Tissue culture using young terminal buds of Aquilaria crassna Pierre ex Lecomte adult trees as explants

[0018] 1. Disinfection of explants and induction of adventitious buds: During the growing season, select the young terminal buds of the vigorous and excellent Chinese Agarwood adult tree as explants, soak them in 75% alcohol by volume for 10 seconds, and then use the mass fraction Disinfect with 0.1% mercuric chloride solution for 5 minutes, rinse with sterile water for 5 times, and then inoculate into adventitious bud induction medium for cultivation. Transfer to a new adventitious bud induction medium for the same culture, and then cultivate for about 30 days to form adventitious buds on the explants. The adventitious bud induction medium contains per liter: 6-benzylpurine 0.5mg, sucrose 20 grams, agar 6g, the remainder is the improved MS medium, pH5.8, and the described improved MS medium is to change the ammonium nitrate in the original MS medium int...

Embodiment 2

[0022] Example 2: Tissue culture using young stem nodes of Aquilaria crassna Pierre ex Lecomte adult trees as explants

[0023] 1. Disinfection of explants and induction of adventitious buds: During the growing season, select the young stem nodes of the vigorous and excellent Agarwood adult tree as explants, soak them in 75% alcohol by volume for 20 seconds, and then use the mass fraction Disinfect with 0.1% mercuric chloride solution for 8 minutes, wash with sterile water 4 times, inoculate into adventitious bud induction medium, culture temperature 26°C, light intensity 1800lx, light 14 hours / day, transfer to new adventitious bud induction culture after 25 days Adventitious buds can be formed on the explants after about 28 days of further culture. The adventitious bud induction medium per liter contains: 0.75 mg of 6-benzylpurine, 25 grams of sucrose, and 6 g of agar, and the balance is the improved MS culture base, pH5.9, the improved MS medium is to change the ammonium ni...

Embodiment 3

[0027] Example 3: Tissue culture using semi-lignified stem nodes of adult trees of Aquilaria crassna Pierre ex Lecomte as explants

[0028] 1. Disinfection of explants and induction of adventitious buds: During the growing season, select the semi-lignified stem nodes of the vigorous and excellent adult trees of Agarwood chinensis as explants, soak them in 75% alcohol for 30 seconds, and then use mass Sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water 5 times, inoculate into adventitious bud induction medium, cultivate at 28°C, illuminance 2000lx, light 16 hours / day, and transfer to new adventitious bud induction after 25 days Culture medium, after culturing for about 25 days, adventitious buds can be formed on the explants. The adventitious bud induction medium contains per liter: 1 mg of 6-benzylpurine, 30 grams of sucrose, 6 g of agar, and the balance is an improved MS medium, pH 6.0. The improved MS medium is the original The ammonium ...

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Abstract

The invention discloses a rapid propagation method for high quality seedlings of Kyara. According to the invention, tender shoots, tender stems or semi-lignification stems of an excellent Kyara adult tree are taken as an explants, after stages of adventitious bud inducement, multiple shoots propagation, rooting and strengthening, transplanting and the like, culture medium formulas and culture conditions, which are suitable for cultivation stages of multiple-shoot inducement, propagation, rooting, transplanting, and the like, are selected, tissue cultivation and quick propagation of Kyara seedings can be achieved, high quality Kyara seedings which meet the demands of market can be bred, so as to be favorable for protection and sustainable utilization of wild resource of Kyara.

Description

Technical field: [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for rapid propagation of high-quality seedlings of Agarwood chinensis. Background technique: [0002] Aquilaria crassna Pierre ex Lecomte (Aquilaria crassna Pierre ex Lecomte), a plant of the genus Aquilaria in the family Thyrrhaceae, is distributed in Cambodia, Laos, Thailand, Vietnam and other places. It is a precious wood tree species that has been introduced and cultivated in my country in recent years. Qinan agarwood has a wide range of uses. In addition to being used as wood and paper, it can also be used to make perfume and cosmetics. After processing, agarwood can also be used as medicine, which has a good effect on nausea, abdominal pain and asthma. With the continuous development of new products using Chinan agarwood as raw materials, the demand for raw materials of Chinaan agarwood is increasing. Unrestricted mining has severely damaged the wild reso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曾宋君陈显鹏陈希吴坤林张建霞段俊
Owner 肇庆丁硕生态技术有限公司
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