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Method for increasing consumption rate of clostridium beijerinckii xylose

A technology of Clostridium beijerinckii and xylose transporter, applied in the field of genetic engineering, can solve the problem of regulating genes without xylose or arabinose metabolic pathways

Inactive Publication Date: 2013-10-30
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Abstract
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  • Application Information

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Problems solved by technology

No xylose or arabinose metabolic pathways and regulatory genes have been studied in Clostridium beijerinckii

Method used

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  • Method for increasing consumption rate of clostridium beijerinckii xylose
  • Method for increasing consumption rate of clostridium beijerinckii xylose
  • Method for increasing consumption rate of clostridium beijerinckii xylose

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Embodiment 1

[0125] In the following examples of the present invention, the xylR gene of the bacterial strain Clostridium beijerinckii NCIMB8052 used is determined by this experiment, and its query number in the NCBI nucleic acid database is gi:149903712; the xylT gene of the bacterial strain Clostridium beijerinckii NCIMB8052 used is determined by this experiment. Confirmed by experiments, its query number in the NCBI nucleic acid database is gi:149901466.

[0126] In the following examples of the present invention, ABE is the abbreviation of acetone+butanol+ethanol (Acetone-butanol-ethanol), and the concentration of ABE refers to the total concentration of acetone, butanol and ethanol in the solution.

[0127] In the following examples of the present invention, the recombinant plasmid vector pIMP1-P ptb It refers to the recombinant plasmid vector expressing xylT gene, and the ptb promoter is derived from the promoter of Clostridium acetobutylicum ATCC824ptb gene.

[0128] In the followi...

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Abstract

The invention discloses a method for increasing the consumption rate of clostridium beijerinckii xylose. Through knocking out transcriptional regulation factor xy1R of the clostridium beijerinckii xylose and clostridium araR and / or increasing the activity of a xylose transport protein, the clostridium can consume higher-concentration xylose and / or arabinose in a culture medium, so that higher-concentration products such as butanol, acetone and ethanol can be produced.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for improving the xylose and / or arabinose consumption rate of Clostridium beijerinckii. Background technique [0002] Due to the limitation of petroleum resources and the volatility of international crude oil prices, the use of renewable resources to produce bio-energy has attracted widespread attention from all over the world. Due to its excellent combustion characteristics and better storage and transportation characteristics than ethanol, butanol is expected to become a new generation of biofuel in the future. Since World War II, with the development of the petroleum industry, the large-scale fermentation of bio-butanol fermentation has been stagnant due to its high raw material cost, and with the continuous rise of food prices in recent years and the formulation of relevant national food security strategies, The use of grain fermentation to p...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74C12P7/28C12P7/06C12P7/16C12R1/145
CPCC12P7/28Y02E50/16C12P7/36C12P7/16C07K14/33Y02E50/17C12P7/10C12P7/06C12R1/145Y02E50/10
Inventor 杨晟肖晗顾阳李治林蒋宇陈军董枫姜卫红
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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