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A method for constructing the pharmacological mechanism of Jinlong Capsules

A technology of Jinlong Capsule and its mechanism, which is applied in the field of traditional Chinese medicine and systems biology, and can solve the problem of single means of pharmacological and pharmacodynamic research

Active Publication Date: 2015-11-18
BEIJING JIANSHENG PHARMA
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Problems solved by technology

[0009] For this reason, the technical problem to be solved by the present invention lies in the fact that in the prior art, the pharmacological and pharmacodynamic research methods of traditional Chinese medicines, especially compound Chinese medicines, are too single, and then provide a method for constructing the pharmacological mechanism of Jinlong Capsules by means of omics and systems biology. method

Method used

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  • A method for constructing the pharmacological mechanism of Jinlong Capsules
  • A method for constructing the pharmacological mechanism of Jinlong Capsules
  • A method for constructing the pharmacological mechanism of Jinlong Capsules

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Effect test

Embodiment 1

[0052] Fifty-five female nude mice aged 5-6 weeks were taken, and the U87-RFP tumor tissue obtained from the subcutaneous tissue of syngeneic mice was cut into 1mm by surgical orthotopic transplantation. 3 Using surgical in situ inoculation technique, the tumor mass was planted into the nude mouse skull, and U87 cells were transfected by constructing a red fluorescent protein-labeled plasmid, and then the U87 cells were injected into the brain to establish an orthotopic brain tumor model. Orthotopic mouse model of human brain tumors expressing red fluorescent protein (U87-RFP).

Embodiment 2

[0054] 96mg / kg Temozolomide, 0.75g / kg Jinlong Capsules, 1.5g / kg Jinlong Capsules and the blank group were administered respectively for 28 consecutive days. The FluorVivoModel-100 fluorescence imager was used for non-invasive real-time imaging observation, and the tumor volume and weight were recorded.

[0055] On the 15th day after administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules, fluorescence in vivo imaging was performed to observe the size of intracranial tumors in nude mice. Such as figure 1 As shown, 96mg / kg temozolomide, 0.75g / kg Jinlong capsule and 1.5g / kg Jinlong capsule have different degrees of inhibition compared with the model group.

[0056] Continuous administration of 96 mg / kg Temozolomide, 0.75 g / kg Jinlong Capsules and 1.5 g / kg Jinlong Capsules was administered for 28 days, and intracranial tumor tissues of nude mice were taken for weighing on the 28th day. Such as figure 2 As shown, compared with the model...

Embodiment 3

[0058] Take 3 brain tumor tissues of the blank control group and 3 brain tumor tissues of the 0.75g / kg Jinlong Capsule group, extract the total RNA of the samples, and use RT-PCR method to reverse transcribe and synthesize cDNA (using RT-PCR kit, Invitrogen Company), Biotin-labeled cRNA was synthesized by in vitro transcription, and the cRNA was purified. After the cRNA was fragmented, it was hybridized with the HumanGenomeU133Plus2.0 gene chip of Affymetrix Company.

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Abstract

The invention belongs to the traditional Chinese pharmacology field, and concretely relates to a method for constructing the pharmacological mechanism of a Jinlong capsule through a biological and genetic engineering process. The method analyzes the system biology of a processed gene chip result and constructs the pharmacological mechanism of the Jinlong capsule by utilizing a Thomson Reuters Genego database through the hybridization, detection and analysis of the gene chip via omics and the system biology process. The invention also discloses a use of the Jinlong capsule as a brain tumor resistance medicine and in chemotherapeutic drug tolerance activity reversion. And the pharmacological mechanism of the Jinlong capsule as the brain tumor resistance medicine and in the chemotherapeutic drug tolerance activity reversion is constructed through the method.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and systems biology, and specifically relates to a method for constructing the pharmacological mechanism of Jinlong Capsule by means of biological and genetic engineering. Background technique [0002] Traditional Chinese medicine, or traditional Chinese medicine, is a unique medicine in traditional Chinese medicine. It has a long history and has a remarkable effect in clinical use for thousands of years. Fresh medicine is an important part of traditional Chinese medicine. Because it contains rich natural active ingredients, it is highly praised for its unique curative effect in the prevention and treatment of certain diseases, especially critical and severe diseases. Traditional fresh medicines are often used immediately, but their clinical use is limited due to the influence of environmental and geographical factors. With the successful research and development of "low-temperature fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00C12Q1/68C12Q1/02A61K35/583A61K9/48A61P35/00
Inventor 黄卉曲育莹岳贵娟李建生
Owner BEIJING JIANSHENG PHARMA
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