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High-flux trace liquid sample distribution device and use method

A technology of trace liquid and dispensing device, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problem of limited throughput of suction and transfer, heavy workload of operators, and expensive pipetting workstations, etc. problem, to achieve the effect of simple operation and low cost

Active Publication Date: 2013-11-20
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in large-scale high-throughput analysis and screening work, there are mainly two ways to distribute liquid samples: one uses multi-channel pipettes to manually absorb and distribute a large number of liquid samples; Generally, there are only 8 channels, 12 channels and 16 channels in the liquid container, and the throughput of each operation is limited. For high-throughput analysis and screening experiments, the workload of operators is still large and the efficiency is low; The other is to use a liquid pipetting workstation to realize the automatic suction and distribution of a large number of liquid samples by means of manipulators. However, the liquid pipetting workstation is expensive, which is often unaffordable for ordinary laboratories, and the liquid pipetting workstation requires specialized technicians to operate, which also increases The difficulty and labor cost of its application
In addition, the above two methods are limited by the size of the pipette tip, and the density of the finally distributed liquid sample array cannot be very high. Good application in analysis and screening

Method used

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  • High-flux trace liquid sample distribution device and use method
  • High-flux trace liquid sample distribution device and use method
  • High-flux trace liquid sample distribution device and use method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0019] Align the fixed plate (2) embedded with the capillary array (1) with the reservoir microwell array plate (3) containing different nucleic acid probes or protein solutions, and move down vertically so that the lower ends of all capillaries are immersed in the reservoir solution In the storage liquid corresponding to the micropore of the device, after the liquid sample fills the capillary by capillary action (such as figure 1 shown), the capillary array filled with liquid samples was transferred to the surface of the nitrocellulose membrane (4), and the lower end of the capillary array was in direct contact with the surface of the nitrocellulose membrane, and the stronger capillary force of the cellulose membrane was used to draw the capillary The liquid is transferred to the substrate to make a low-density gene microarray chip or protein microarray chip (such as figure 2 shown).

Embodiment 2

[0021] Align the fixed plate (5) embedded with capillary array (1) and integrated driving function with the reservoir microwell array plate (3) containing different protein crystallization reagents, and move down vertically so that the lower ends of all capillaries are immersed in the reservoir. In the protein crystallization reagent corresponding to the micropore of the liquid container, after the liquid sample fills the capillary by capillary action (such as image 3 shown), transfer the capillary array filled with liquid sample to the top of the liquid sample receiving microwell array plate (6), and compress the air at the upper end of the capillary array through the injection head or hollow rubber ball, and make the liquid in the capillary tube spray from the lower end through the pulse pressure out, complete the distribution of different crystallization reagents in the microwell array plate (such as Figure 4 shown); then, use another capillary fixing plate with integrate...

Embodiment 3

[0023] Align the fixed plate (2) embedded with the capillary array (1) with the reservoir microwell array plate (3) containing different drug solutions, and move down vertically so that the lower ends of all the capillaries are immersed in the corresponding microwells of the reservoir In the drug solution, after the drug solution fills the capillary by capillary action (such as Figure 7shown), the capillary array filled with liquid sample is transferred to the top of the liquid sample receiving microfluidic chip (8), and the lower end of the capillary array is aligned with the microfluidic chip injection microwell array, and inserted into it; The central injection port of the flow control chip is then dripped with the bacterial solution (such as Figure 8 shown); after that, since each sample port of the microfluidic chip is attached with a vacuum-degassed PDMS micropump (9), the pump absorbs the air in the micropipe in the chip to generate a negative pressure, and the drug s...

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Abstract

The invention discloses a high-flux trace liquid sample distribution device. The device comprises a group of capillary tube arrays which are arrayed in parallel, a capillary tube array fixation frame, and a liquid storage device corresponding to the capillary tube arrays. The device is used for realizing automatic high-flux trace absorption of a plurality of liquid samples simultaneously via a capillary action, and transferring liquid in each capillary tube into a liquid sample receiving substrate, a microporous plate or a microfluid chip by keeping the lower end of the capillary tube in contact with the substrate with a strong capillary action or compressing air at the upper end of the capillary tube or performing negative pressure suction on the lower end of the capillary tube, thereby finishing high-flux distribution of the liquid samples, wherein the volumes of the distributed liquid samples are decided by the inner diameters and lengths of the capillary tubes. The method and the device are capable of realizing high-flux, accurate and rapid distribution of the extreme trace samples, can be applied to chemical or biological large-scale detection and screening experiments, and are capable of greatly saving labour and time and increasing experiment efficiency.

Description

technical field [0001] The invention relates to a high-throughput micro liquid sample distribution device, which can be applied to the fields of chemical and biochemical high-throughput analysis and screening. Background technique [0002] As we all know, the research of chemistry and life science is mainly based on liquid manipulation. With the in-depth development of modern chemistry and biology, there has been a clear trend that more and more experiments are involved, such as combinatorial chemistry, lead synthetic chemistry, proteomics research, DNA sequencing and analysis, drug synthesis and screening etc., all of which involve the sampling and distribution of large volumes of liquid samples. As the number of tests increases, so does the workload of quantitative aspiration and transfer of liquids. At present, in large-scale high-throughput analysis and screening work, there are mainly two ways to distribute liquid samples: one uses multi-channel pipettes to manually a...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 李刚廖玲英陈强赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI