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Method and kit for leukocyte differential count

A white blood cell classification and kit technology, applied in the biological field, can solve the problems of white blood cell classification and counting that interfere with cell morphology analysis technology, staining methods are not suitable for manual identification, etc., and achieve the effect of maintaining integrity, good effect and accurate results

Active Publication Date: 2015-06-24
AVE SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, after the above-mentioned special reagents destroy RBC, red blood cell fragments will be left behind. These fragments will not affect the detection of blood cell analyzers based on the counting principle of flow cytometry or electrical impedance, but will interfere with the white blood cells of the cell morphology analysis technology based on the principle of machine vision. Classification and counting, and the staining methods of these reagents are not suitable for manual identification under ordinary light microscopes

Method used

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  • Method and kit for leukocyte differential count
  • Method and kit for leukocyte differential count

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Experimental program
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Effect test

Embodiment 1

[0090] Embodiment 1: kit of the present invention

[0091] Reagent A composition and concentration:

[0092] 5g / L NaCl inorganic salt, 0.02M by Na 2 HPO 4 , NaH 2 PO 4 Composition buffer, 4mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.1g / L KH 2 PO 4 , 0.15g / L methylene blue dye, 0.1mL / L glutaraldehyde stabilizer;

[0093] Reagent B composition and concentration:

[0094] 5g / L NaCl inorganic salt, 0.02M by Na 2 HPO 4 , NaH 2 PO 4 Composition buffer, 4mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.1g / L KH 2 PO 4 , 0.5g / L eosin Y dye, 0.1mL / L glutaraldehyde stabilizer.

Embodiment 2

[0095] Embodiment 2: kit of the present invention

[0096] Reagent A composition and concentration:

[0097] 3g / L NaCl inorganic salt, 0.02M by Na 2 HPO 4 , NaH 2 PO 4 Composed buffer, 5mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.14g / L KH 2 PO 4 , 0.1g / L methylene blue dye, 0.2mL / L glutaraldehyde stabilizer;

[0098] Reagent B composition and concentration:

[0099] 3g / L NaCl inorganic salt, 0.02M by Na 2 HPO 4 , NaH 2 PO 4 Composed buffer, 5mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.14g / L KH 2 PO 4 , 0.4g / L eosin Y dye, 0.2mL / L glutaraldehyde stabilizer.

Embodiment 3

[0100] Embodiment 3: kit of the present invention

[0101] Reagent A composition and concentration:

[0102] 6.5g / L NaCl inorganic salt, 0.02M Na 2 HPO 4 , NaH 2 PO 4 Composed buffer, 5mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.14g / L KH 2 PO 4 , 0.1g / L methylene blue dye, 0.1mL / L glutaraldehyde stabilizer;

[0103] Reagent B composition and concentration:

[0104] 6.5g / L NaCl inorganic salt, 0.02M Na 2 HPO 4 , NaH 2 PO 4 Composed buffer, 5mL / L octylphenyl polyoxyethylene ether hemolytic agent, 0.14g / L KH 2 PO 4 , 0.36g / L eosin Y dye, 0.1mL / L glutaraldehyde stabilizer.

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Abstract

The invention relates to the field of biotechnology, and discloses a method and a kit for leukocyte differential count. The kit comprises a reagent A and a reagent B, wherein each of the reagent A and the reagent B respectively comprises the components selected from proper dye, a buffer agent, a hemolytic agent, inorganic salt, acid salt and a stabilizer; after the reagent A and the reagent B are mixed with a blood sample, erythrocyte can be rapidly and thoroughly damaged, so that erythrocyte fragments can be effectively dissolved; meanwhile, a leukocyte membrane is punched, so that the dye can rapidly enter the leukocyte cytoplasm and can be combined with different particles in the leukocyte cytoplasm so as to take on different colors; furthermore, the leukocyte membrane is protected by other reagents in a synergetic manner, so that the integrity of the leukocyte can be maintained; therefore, an instrument has a good effect of distinguishing the different types of leukocyte, and is especially suitable for automatically sorting the leukocyte by a machine vision principle-based cell morphology analysis technology.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and kit for differential counting of white blood cells. Background technique [0002] White blood cells, also known as white blood cells, are one of the blood cell components. They are colorless round cells, slightly larger than red blood cells, and have nuclei. Can be divided into neutrophils, eosinophils, basophils, lymphocytes and monocytes five. When inflammation or disease occurs in the body, the number of white blood cells often changes, so it is one of the methods for disease inspection and diagnosis. For example, neutropenia is seen in acute infection, uremia, acute bleeding from severe burns, tissue damage, etc.; its decrease is seen in typhoid and paratyphoid, aplastic anemia, acute agranulocytosis, hypersplenism, etc. Increased eosinophils are seen in allergic reactions; reductions are seen in typhoid, paratyphoid, and after the use of adrenal corticosteroids. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10
Inventor 钟志宏丁建文
Owner AVE SCI & TECH CO LTD