Sample analysis system

A sample analysis device and sample technology, which can be used in analytical materials, laboratory containers, instruments, etc., and can solve problems such as complex operation and cost

Active Publication Date: 2013-11-20
AKONNI BIOSYSTEMS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microarrays have not been widely used for molecular testing in clinical laboratories because of their operational complexity and cost (often costing hundreds of dollars per test)

Method used

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Examples

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example 2

[0076] Example 2: Multiplexer Design

[0077] This example involves the testing and design process of a device for selecting between 8 different ports of an 8-port manifold to allow air to flow through only a single port at a time. The device, called an 8-way selector, was used to dry pipette tips on an automated liquid handling system. The system utilizes 8 pipette tips to simultaneously complete eight separate sample preparations. In one embodiment, an 8-way selector is designed to allow air flow from a common air source to dry substrates within pipette tips.

[0078] A. Detection of flow rate

[0079] Before integrating the 8-way selector into the 8-port manifold, tests were performed to determine the effect of air flow rate on crossing threshold (CT) in the DNA sampling and amplification process used. Briefly, the system is connected to a flow meter to measure flow. Test the effect of 5 different new flow rates on CT values ​​during DNA extraction and amplification. T...

example 3

[0085] Example 3: Automated multi-sample detection system

[0086] Figure 9 An automated sample-response system 900 is shown capable of performing sampling, slide PCR, and array imaging for 8 samples simultaneously.

[0087] A. Sample purification / extraction

[0088] System 900 has three major subsystems involved in sample purification and extraction. These three subsystems include tip holder 910 , plate holder 920 and plunger system 930 . Tip holder 1100 secures TruTips (not shown) to system 900 and keeps them stationary in the X-Y plane. However, the tip holder 910 is connected to an actuator that allows the TruTip to be controlled in the Z-plane. It is also conceivable to have the TruTip move in all directions (ie not be stationary). The plate holder 920 holds a 2 mL 96-deep well plate 921 which is used as a reservoir for all reagents and samples required for the end-to-end run. The plate holder 920 moves the well plate 921 in the X-Y plane, allowing the TruTip to mo...

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Abstract

An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation / analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.

Description

[0001] This application claims priority to US Provisional Patent Application No. 61 / 421,414, filed December 9, 2010. The above application is incorporated herein by reference in its entirety. technical field [0002] The present application relates generally to sample analysis systems and, in particular, to an integrated sample-to-answer analysis system for detecting biological material in a sample. Background technique [0003] Molecular testing is an assay performed at the molecular level to detect biological material such as DNA, RNA and / or proteins in the sample being tested. Molecular assays have begun to represent the gold standard for their speed, sensitivity, and specificity. Molecular tests, for example, were found to be more than 75% more sensitive than traditional culture methods for identifying enteroviruses in cerebrospinal fluid, and are now considered the gold standard for this diagnostic method (Leland et al., Clin. Microbiol Rev. 2007 , 20:49-78). [0004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/48G01N35/08
CPCB01L2300/0636B01L2200/04B01L2300/0829B01L2300/044B01L2200/16B01L2400/0683B01L2300/0672B01L2300/087B01L3/50855B01L7/52B01L2300/0819B01L3/5025B01L2400/0487B01L3/502715B01L2200/0678B01L2300/0681B01L2400/065
Inventor 克里斯托弗·G·库尼菲利普·贝尔格雷德尔阿里亚·布鲁诺斯蒂夫·加柏尼图·H·特科雷彼得·Q·曲
Owner AKONNI BIOSYSTEMS INC
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