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Method for screening candidate plasma protein markers by cervical carcinoma specificity difference expression

A technology of differential expression and screening method, which is applied in the field of molecular biology or medical diagnosis, and can solve problems such as blank research reports on cervical cancer and limited number of genes

Inactive Publication Date: 2013-11-27
XINJIANG MEDICAL UNIV
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Problems solved by technology

At that time, the human genome-wide expression profiling chip used by people contained a limited number of genes, and there was almost no research report on cervical cancer in Uighur women in Xinjiang

Method used

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  • Method for screening candidate plasma protein markers by cervical carcinoma specificity difference expression
  • Method for screening candidate plasma protein markers by cervical carcinoma specificity difference expression
  • Method for screening candidate plasma protein markers by cervical carcinoma specificity difference expression

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Embodiment Construction

[0022] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0023] A screening method for a candidate plasma protein marker specifically and differentially expressed in cervical cancer, comprising the following steps:

[0024] (1) Clinical specimen collection and tissue RNA preparation

[0025] From 2010 to 2011, based on the inpatient clinic or gynecology clinic of the Third Affiliated Tumor Hospital of Xinjiang Medical University, Uighur women’s cervical squamous cell carcinoma (CSCC) and cervical intraepithelial neoplasia (CIN) II -III and normal control (NC) as subjects, systematically collect patient case information and fresh cervical tissue samples, take out the samples on the spot, immediately freeze and transport them in liquid nitrogen, and store them in a -80°C ultra-low temperature freezer. From the above tissue samples, 72 cases of pathologically confirmed tissue samples were select...

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Abstract

The invention discloses a method for screening candidate plasma protein markers by cervical carcinoma specificity difference expression. The method comprises the following steps: (1)collecting clinical specimens, and preparing tissue RNA (Ribonucleic Acid); (2) analyzing a uyghur woman cervical carcinoma and precancerous lesion tissue RNA high-density human gene expression profile chip; and (3) carrying out semiquantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) analysis on cervical carcinoma specificity difference expression candidate genes. The method disclosed by the invention discovers that the expression level of mRNA (Messenger RNA) encoded by most of candidate genes has outstanding difference between CSCC (Cervical Sqamous Cell Carcinoma) (or CIN (Cervical Intraepithelial Neoplasia)) and NC tissues through semiquantitative PCR (Polymerase Chain Reaction) screening identification and proves the reliability and sensitivity of the analytical data of the gene chip.

Description

technical field [0001] The patent of the invention belongs to the technical field of molecular biology or medical diagnosis, and relates to a screening method for specific and differentially expressed candidate plasma protein markers of cervical cancer. Background technique [0002] Cervical cancer is a gynecological malignancy, and early detection is the key to effective tumor treatment. The morbidity and mortality of cervical cancer among Uighur women are very high, and it is listed as an extremely high incidence tumor in Xinjiang. Due to the inherent limitations of existing tumor diagnosis techniques, most of the tumor patients admitted to the clinic are in the middle and advanced stages, and the treatment effect is poor. Tumor-specific differentially expressed genes can become early warning molecular markers closely related to tumor occurrence, and are an important prerequisite for establishing early molecular diagnostic methods for tumors. The technology related to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 阿布力孜·阿布杜拉刘开江郭霞阿仙姑·哈斯木阿比达·阿不都卡德尔阿比班·艾库拉阿丽叶古丽·艾皮热吐尔逊帕夏·吾布力卡斯木希尔扎提·苏莱曼玛依拉·卡米力江武贵臻古丽扎热叶·阿库拉布威海丽且姆·阿巴拜科日
Owner XINJIANG MEDICAL UNIV
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