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Inducing method for spring dendrobium protoemm-like body

A protocorm-like and Dendrobium-like technology, applied in the induction field of Dendrobium-like protocorms, can solve the problems of cumbersome conditions, complex induction medium components, long cycle, etc., and achieve high proliferation efficiency and great application value

Inactive Publication Date: 2013-12-04
ZHEJIANG COLLEGE OF CONSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The composition of the induction medium used in this method is complex, and the conditions of induction culture are relatively cumbersome and the cycle is long. The key is that the obtained protocorm does not have the germplasm characteristics consistency of clonal propagation.
So far, there have been no successful reports on the induction of protocorm-like regeneration by plant materials in Dendrobium officinale

Method used

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  • Inducing method for spring dendrobium protoemm-like body
  • Inducing method for spring dendrobium protoemm-like body
  • Inducing method for spring dendrobium protoemm-like body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]A method for rapid propagation of dendrobium tissue culture, comprising:

[0038] (1) The stem tips or axillary buds of the Dendrobium Sanya cultivar Dendrobium Sanya were selected as explants to induce sterile seedlings;

[0039] Rinse the stem tip or the stem section with axillary buds with running water, treat with 70% ethanol for 1 min, 0.1% mercury chloride for 7-10 min, insert it into the basic medium, and induce Cultivate for about 30 days to obtain sterile vaccines (such as figure 1 shown);

[0040] The basic medium is solid 1 / 2MS medium containing 1.0mg / L6-BA and 0.2mg / L NAA;

[0041] (2) Take the leafless bud stem section of the stem base of the sterile seedling, inoculate it in the induction medium after ultrasonic treatment, and induce culture under the condition of light (6000-12000lx) at 25-28°C for 30-60 days to obtain protocorm-like ( Such as figure 2 shown);

[0042] Cut the leafless bud stem section of 3mm sterile seedling base as the induction ma...

Embodiment 2

[0053] The same method as in Example 1 was used, but step (2) was not subjected to ultrasonic treatment. Protocorm-like was not induced.

Embodiment 3

[0055] The same method as in Example 1 was used, but in step (2) 40KHz ultrasonic treatment was used for 15min. Within a week, almost all the materials died, and no protocorms were obtained.

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Abstract

The invention discloses an inducing method for spring dendrobium protoemm-like body, which comprises the following steps: taking a tender tissue of a spring dendrobium plant as an explant, and carrying out inducing to obtain a germ-free seedling; inoculating a non-bud stem of the caudex of the germ-free seedling into an inducing culture medium after treatment of ultrasonic waves, and carrying out inducing cultivation to obtain the spring dendrobium protoemm-like body. As the non-bud stem of the caudex of the germ-free seedling is taken as an inducing material, and the generation of the protoemm-like body is induced under the assistance of ultrasonic wave treatment, a new important way is provided for the spring dendrobium tissue culture and fast propagation technology; the protoemm-like body obtained according to the method not only retains the favorable property of an original variety, but also is high in pullulation efficiency which is 2 to 3 times of that of cluster buds, and the application value in production is high; as the protoemm-like body can serve as an acceptor material of spring dendrobium transgenic breeding while cluster buds cannot, so that more transgenic spring dendrobium varieties can be obtained through the protoemm-like body.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing protocorms of Dendrobium officinalis. Background technique [0002] Spring Dendrobium, also known as Meihua Dendrobium, is named for its spring flowering. It is a perennial epiphytic orchid plant of the genus Dendrobium orchidaceae. Compared with the common autumn Dendrobium, it has a compact plant shape, many flowers, bright colors, fragrance and easy management. Extensive and other characteristics, it is a popular high-end potted flower at home and abroad. [0003] Spring Dendrobium seedlings are mainly propagated by tissue culture and cuttings. Due to the fast propagation speed and high reproduction coefficient of tissue culture, it is especially suitable for detoxification and promotion of new varieties, so it plays a leading role in the production of high-quality seedlings of Spring Dendrobium. [0004] Most of the existing tissue culture ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郑泉
Owner ZHEJIANG COLLEGE OF CONSTR
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