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Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof

A technology of glass chips and cell arrays, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of not being able to obtain single cell arrays, not being able to limit the location and shape of cell growth, and not being able to form a closed cell Regional and other issues, to achieve the effect of simple and easy to operate in the preparation process, easy to control parameters, and high economy

Inactive Publication Date: 2015-01-28
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The following problems exist in the prior art: the cell growth substrate is chemically modified to promote cell adhesion based on microfluidic patterning technology, and what is obtained is a strip-shaped modified pattern that is the same as the planar structure of the microchannel network, and cannot form a closed area. Therefore, The surface modification obtained by this method cannot restrict the growth location and shape of cells, and cannot obtain single-cell arrays

Method used

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  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof
  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof
  • Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof

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Embodiment 1

[0026] The chip in this example is used to culture a single osteoblast MC3T3-E1 for cell contact connection research. MC3T3-E1 average diameter It is 20μm.

[0027] Refer to Figure 1 ~ Figure 3 . In this example, the single-cell array culture glass chip is used for MC3T3-E1 cell culture. Among them, the structure size of the single cell array culture glass chip is as follows: the size of the cell growth area 1 a=b=50μm; the lateral distance between the center lines of two adjacent cell growth areas 1 w1=70μm; the growth of two adjacent cells The longitudinal distance between the center lines of area 1 w2=80μm; the line width of PHEMA hydrogel pattern 2 is d=20μm, the size of the convex part of the hydrogel pattern c=e=20μm; the distance between two adjacent PHEMA hydrogels is the most The narrow distance h=20μm.

[0028] In this embodiment, the method for manufacturing a single-cell array culture glass chip based on microfluidic patterning technology is implemented by using M...

Embodiment 2

[0037] The chip in this example is used to cultivate human breast epithelial cells HMEC to study the growth characteristics of the cells. HMEC average diameter It is 45μm.

[0038] Refer to Figure 1 ~ Figure 3 . In this example, a single cell array culture glass chip is used for HMEC cell culture. Among them, the structure size of the single cell array culture glass chip is as follows: the size of the cell growth area 1 a=b=80 μm; the lateral distance between the center lines of two adjacent cell growth areas 1 w1=120 μm; the growth of two adjacent cells The longitudinal distance between the center lines of area 1 w2=140μm; the line width of the PHEMA hydrogel pattern d=40μm, the size of the convex part of the hydrogel pattern c=e=40μm; the distance between two adjacent PHEMA hydrogels The narrow distance h=20μm.

[0039] In this embodiment, the method for manufacturing a single-cell array culture glass chip based on microfluidic patterning technology is implemented by using ...

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Abstract

The invention discloses a glass chip for cultivating a single cell array based on microfluidic patterning technology, and a preparation method thereof, and belongs to the field of biological micro electro mechanical systems. The glass chip adopts the structure that a layer of PHEMA hydrogel pattern 2 is arranged on a glass base 4, and divides the glass base 4 into a plurality of rectangular cell growth zones 1 to generate the single cell array. The preparation process is that graphical PHEMA processing is performed on the surface of a glass sheet through a PDMS elastic stamp. The glass chip has the benefits that the stable surface chemical modification is adopted, so that hydrogel dressings capable of resisting cell adhesion is arranged on the surface of the glass in a graphical manner to form the cell graphical surface containing cell adhesion and resisting cell adhesion so as to cultivate the single cell array; the preparation process is simple and easy to operate; when the chip is applied to cell culture, the single cell growth array is obtained, and a new tool for studying basic cell biology is provided.

Description

Technical field [0001] The invention relates to a single cell array culture glass chip structure and a preparation method based on microfluidic patterning (microfluidic patterning, uFLP), and belongs to the field of Bio-MEMS (Bio-MEMS). Background technique [0002] Cell culture is one of the most important basic sciences in the fields of biology, medicine, and new drug development. In the past two hundred years, cell culture has made great contributions to vaccine development, genetic analysis, and biological research. With the development of modern biochemistry, molecular biology, molecular genetics, and modern medicine, cell culture has also fully demonstrated its huge development potential in many application fields. There are many conventional culture methods for adherent cells, which are characterized by cell population culture, such as cell culture flasks, cell culture dishes, and cell culture plates. However, the above-mentioned cell culture methods all have the followi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 高洁叶芳马炳和魏晨谢丽郝艳鹏谢晋
Owner NORTHWESTERN POLYTECHNICAL UNIV
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