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Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester

A technology of glucose and microbial strains, applied in the field of biochemical engineering, can solve the problems of long fermentation time, poor passage stability, and unsuitable for industrial production.

Inactive Publication Date: 2013-12-18
防城港市润杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used production method of glucose-6-acetate is obtained by using microbial fermentation method, and the strain used is ATCC10778, but the yield of this strain is not high, the fermentation time is long and the passage stability is poor, so it is not suitable for large-scale industrial production

Method used

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  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester
  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester
  • Strain capable of massively yielding glucose-6-acetic ester and method for synthesizing glucose-6-acetic ester

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0057] (1) Inclined cultivation of strains

[0058] Inoculating the Bacillus sp. strain GXU-011 strain into the slant medium in the test tube to make the slant strain;

[0059] Among them, the slant culture medium of bacterial species is composed of the following parts by weight:

[0060] 40g sucrose, 2g yeast powder, KH 2 PO 4 0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, 15g agar powder and 1000 parts of water;

[0061] The technological conditions for preparing the slant strains were pH 6.5, temperature 28°C, and culture time 24 hours.

[0062] (2) Inoculation and fermentation

[0063] Expand the cultivation of slant strains into seed liquid;

[0064] Connect the seed liquid to the fermentor at 5% of the inoculum for fermentation;

[0065] The seed liquid and fermentation liquid are composed of the following parts by weight:

[0066] 40 parts glucose, 5 parts peptone, 5 parts yeast powder, 1 part...

Embodiment 2

[0076] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0077] (1) Inclined cultivation of strains

[0078] Inoculating the Bacillus sp. strain GXU-011 strain into the slant medium in the test tube to make the slant strain;

[0079] Among them, the slant culture medium of bacterial species is composed of the following parts by weight:

[0080] Cane sugar 50g, yeast powder 6g, KH 2 PO 4 2g, K 2 HPO 4 2g, MgSO 4 2g, 20g agar powder and 1000 parts of water;

[0081] The technological conditions for preparing the slant strains were pH 7.0, temperature 30°C, and culture time 30 hours.

[0082] (2) Inoculation and fermentation

[0083] Expand the cultivation of slant strains into seed liquid;

[0084] Connect the seed liquid to the fermenter at 8% of the inoculum for fermentation;

[0085] The seed liquid and fermentation liquid are composed of the following parts by weight:

[0086] 50 parts of glucose, 8 parts of peptone, 8 parts of yeast powder, ...

Embodiment 3

[0093] The steps of the fermentation separation method for producing glucose-6-acetate are as follows:

[0094] (1) Inclined cultivation of strains

[0095] Inoculating the Bacillus sp. strain GXU-011 into the slant medium in the test tube to make the slant strain;

[0096] Among them, the slant culture medium of bacterial species is composed of the following parts by weight:

[0097] 60g sucrose, 8g yeast powder, KH 2 PO 4 1.5g, K 2 HPO 4 1.5g, MgSO 4 1.5g, 25g agar powder and 1000 parts of water;

[0098] The process conditions for preparing the slant strains were pH 7.5, temperature 32°C, and culture time 36 hours.

[0099] (2) Inoculation and fermentation

[0100] Expand the cultivation of slant strains into seed liquid;

[0101] Connect the seed liquid to the fermenter at 10% of the inoculum for fermentation;

[0102] The seed liquid and fermentation liquid are composed of the following parts by weight:

[0103] 60 parts glucose, 8 parts peptone, 9 parts yeast powder, 2 parts potassium ...

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Abstract

The invention discloses a strain capable of massively yielding glucose-6-acetic ester and a method for synthesizing the glucose-6-acetic ester. The collection number of Bacillus sp. GXU-011 is CGMCC No.7031; the Bacillus sp. GXU-011 is collected in the China general microbiological culture collection center; the collection date is December 24, 2012; the collection address is Beijing institute of microbiology, Chinese Academy of Sciences. The method comprises the following steps of: culturing organic nutrients, namely a carbon source, a nitrogen source, inorganic salt, and the like to obtain reaction liquid glucose-6-acetic ester; then separating and purifying the reaction liquid glucose-6-acetic ester to obtain the glucose-6-acetic ester. Compared with the traditional strain ATCC10778, the strain disclosed by the invention has the advantages of short fermentation time, simple process step, high output and high passage stability.

Description

Technical field [0001] The invention belongs to the field of biochemical engineering, in particular to a strain with high production of glucose-6-acetate and a method for synthesizing glucose-6-acetate. Background technique [0002] The invention relates to the use of biological fermentation to prepare glucose-6-acetate, a key intermediate for the production of sucralose. Glucose-6-acetate is a key intermediate for the production of 4,1',6'-trichloro-4,1',6'-tideoxygalactose, or sucralose. Sucralose is a chloro derivative of sucrose. Its sweetness is 600-800 times that of sucrose. Its taste is mellow, rich, stable, and low in calories. It can be used in conjunction with traditional sweeteners or used alone. Its sweet taste is not affected, which is beneficial to consumers' health. Glucose-6-acetate is a key intermediate in the synthesis of sucralose. At present, the commonly used production method of glucose-6-acetate is obtained by microbial fermentation. The strain used is A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04C12P19/02C12R1/07
Inventor 李群良张欣邓子明
Owner 防城港市润杰生物科技有限公司
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