Biologically-safe quality control material for blood tester and preparation method thereof

A biosafety and tester technology, applied in the direction of biological testing, material inspection products, etc., can solve the problem of unsatisfactory shelf life and other problems, and achieve the effect of overcoming changes in cell volume, large controllability, and long shelf life

Active Publication Date: 2013-12-18
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality control substance described in the present invention has better quality control effect, but its shelf life is not ideal enough

Method used

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  • Biologically-safe quality control material for blood tester and preparation method thereof
  • Biologically-safe quality control material for blood tester and preparation method thereof
  • Biologically-safe quality control material for blood tester and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the preparation of the quality control substance of hemoglobin analyzer

[0036] A) Collect goat blood and perform anticoagulant treatment, the anticoagulant is EDTA-K2, and the dosage is 1.5-2.2MG / ml blood for anticoagulant treatment;

[0037] B) centrifuging the goat blood collected above, removing plasma and white blood cells, collecting red blood cells at the bottom, and obtaining red blood cells with a concentration higher than 95%;

[0038] C) Place the collected red blood cells in the cell maintenance solution, adjust the concentration of red blood cells in the cell maintenance solution according to the content of hemoglobin in the quality control substance to be prepared, and obtain the corresponding concentration of hemoglobin control substance; the composition of the cell maintenance solution For: penicillin 0.15g / L, gentamicin 0.15g / L, chloramphenicol 0.1g / L, glucose 2g / L, bovine serum albumin 20g / L, sodium chloride 6.3g / L, sodium citrate 7.8g ...

Embodiment 2

[0039] Embodiment 2: Preparation of the whole blood quality control substance of the three-differentiation hematology analyzer

[0040] A) Collect the blood of dogs, cows and ostriches respectively, and process them with anticoagulant respectively in the process of collection; the anticoagulant is selected EDTA-K 2 , the dosage is 2.0MG / ml blood;

[0041] B) take a part of the collected dog blood and centrifuge, remove the upper layer of plasma and the middle layer of white blood cells, collect the bottom red blood cells, and obtain red blood cells with a concentration of 98.5%;

[0042] C) Mix the collected red blood cells with the maintenance solution, test it with a hematology analyzer, and adjust the RBC count to 8.0X10 with the maintenance solution 12 / L±1.0X10 12 / L, the volume ratio is about 1:1, and stored at 5°C, the red blood cell simulant can be obtained;

[0043] D) Centrifuge the remaining dog blood, collect the red blood cells at the bottom, add 3% (v / v) forma...

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PUM

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Abstract

The invention discloses a biologically-safe quality control material for a blood tester and a preparation method thereof. The preparation method comprises that animal red blood cells having the sizes similar to sizes of human red blood cells are used for simulation of human red blood cells; animal red blood cells having the sizes similar to sizes of large cells in human white blood cells and animal red blood cells have the sizes similar to sizes of small cells in human white blood cells are used for simulation of human white blood cells; animal red blood cells have the sizes similar to sizes of human blood platelets are used for simulation of human blood platelets; the animal red blood cells for simulation of human white blood cells and human blood platelets are cured by formaldehyde having the content of 2-8%; the cured animal red blood cells are cleaned and then are added with bovine serum albumin so that the cure agent is removed; and according to requirements, through blending, the single-component or whole blood quality control material is obtained. The biologically-safe quality control material has good stability, a low cost and high safety, and is suitable for a blood cell analyzer, a urinary sediment analyzer and a hemoglobin analyzer.

Description

technical field [0001] The invention relates to the technical field of blood testing, in particular to a biological safety quality control substance for a blood testing instrument and a preparation method thereof. Background technique [0002] Quality controls are used to check the performance of an analytical instrument or method. When a substance is used in routine tests to check the current performance of analytical instruments or methods, its analytical properties must be similar to those of patient samples. [0003] With the rapid development of medical diagnostic technology, the use of various blood clinical testing instruments is becoming more and more advanced and common, which not only provides more experimental indicators for clinical practice, but also greatly improves the efficiency and accuracy of clinical testing. However, in order to obtain accurate and reliable results and to make the results of different instruments and different laboratories comparable, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 周永杨唐雪辉陈尚斌
Owner URIT MEDICAL ELECTRONICS CO LTD
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