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A method for extracting ursolic acid from rosemary

A technology of rosemary and ursolic acid, applied in the directions of steroids, organic chemistry, etc., can solve the problems of many types of reagents, unsuitable for promotion, cumbersome operation, etc. high content

Active Publication Date: 2016-02-17
盐城市盐龙湖生态风景保护区开发建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many steps, cumbersome operation, many types and large quantities of reagents to be used, and is not suitable for popularization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting ursolic acid from rosemary, the steps are as follows:

[0025] 1) Carry out subcritical extraction of rosemary, the extractant is a fluid composed of acetone and ethanol in a weight ratio of 30:70, the extraction temperature is 55°C, the pressure is 2.6MPa, and the time is 30min, and the extract is taken;

[0026] 2) Add activated carbon with 1% weight of rosemary to the extract, reflux for decolorization for 30 minutes, filter, concentrate the filtrate to 50% of the original volume, let it stand at room temperature, and crystallize, and use 95% ethanol solution with a pH of 11 for the crystals Dissolve to a saturated solution, filter, adjust the pH of the filtrate to 3.5, stand at room temperature, crystallize, filter, wash the crystals with 30% ethanol solution, and dry to obtain the finished product.

[0027] The product yield of the crystal obtained after the first crystallization of the present embodiment is 4.0%, and the ursolic acid weight...

Embodiment 2

[0029] A method for extracting ursolic acid from rosemary, the steps are as follows:

[0030] 1) Carry out subcritical extraction of rosemary, the extractant is a fluid composed of acetone and ethanol in a weight ratio of 25:70, the extraction temperature is 58°C, the pressure is 2.4MPa, and the time is 30min, and the extract is taken;

[0031] 2) Add activated carbon with 1.2% weight of rosemary to the extract, reflux and decolorize for 20 minutes, filter, concentrate the filtrate to 50% of the original volume, let it stand at room temperature, and crystallize, and use 90% ethanol solution with a pH of 11 for the crystals Dissolve into a saturated solution, filter, adjust the pH of the filtrate to 3.5, stand at room temperature, crystallize, filter, wash the crystals with 35% ethanol solution, and dry to obtain the finished product.

[0032] The product yield of the crystal that the present embodiment obtains after crystallization for the first time is 3.5%, and the ursolic a...

Embodiment 3

[0034] A method for extracting ursolic acid from rosemary, the steps are as follows:

[0035] 1) Carry out subcritical extraction of rosemary, the extractant is a fluid composed of acetone and ethanol in a weight ratio of 30:75, the extraction temperature is 52°C, the pressure is 2.6MPa, and the time is 40min, and the extract is taken;

[0036] 2) Add activated carbon with 0.8% weight of rosemary to the extract, reflux for 30 minutes, filter, concentrate the filtrate to 60% of the original volume, let it stand at room temperature, and crystallize, and use 93% ethanol solution with a pH of 10 for the crystals Dissolve into a saturated solution, filter, adjust the pH of the filtrate to 4, stand at room temperature, crystallize, filter, wash the crystals with 25% ethanol solution, and dry to obtain the finished product.

[0037] The product yield of the crystal that the present embodiment obtains after crystallization for the first time is 3.7%, and the ursolic acid weight conten...

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Abstract

The invention discloses a method for extracting ursolic acid from rosmarinus officinalis. The method includes the following steps that 1) subcritical extraction is conducted on the rosmarinus officinalis, and acetone and ethyl alcohol are used as extracting agents; 2) activated carbon with the weight being the same as that of the rosmarinus officinalis is added into an extracting solution, reflux decoloration, filtering, filter liquor concentrating, standing and devitrification are carried out, crystals are dissolved into a saturated solution by using a 90%-95% alkaline ethanol solution, filtering is conducted, the pH of the filter liquor is adjusted to be 3-4, standing, devitrification and filtering are carried out, the crystals are washed by using a 25%-35% ethanol solution, and after drying is completed, a finished product can be obtained. According to the method for extracting the ursolic acid from the rosmarinus officinalis, the subcritical extraction mode is adopted, the advantages of being short in time, few in step and small in dosage of reagents are achieved, after devitrification is conducted on the extracting solution, alkalinity is adjusted, and the alkali using amount is little compared with the mode that devitrification is not conducted and alkalinity is adjusted directly. Meanwhile, the steps are simple, time and labor are saved, the yield of devitrification for the first time is 3.5%-4.0%, the content is 85%-90%, the yield of devitrification for the second time is 3.0%, the content is 98%-99%, the yield and the content are high, and therefore the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of a plant extract, in particular to a method for extracting ursolic acid from rosemary. Background technique [0002] Ursolic acid, also known as ursolic acid and ursolic acid, is a kind of triterpenoid compound existing in natural plants. Biological effects, ursolic acid also has obvious antioxidant function, so it is widely used as raw materials for medicine and cosmetics. [0003] Rosemary (Rosmarinus officinalis) is a perennial evergreen shrub native to the Mediterranean region. It is wild or planted in chalky soil. Its stems, leaves and flowers can extract aromatic oil. Rosemary contains various antioxidant components, such as ursolic acid, carnosic acid, carnosol, rosmanol, rosmarinic acid, etc. [0004] CN101759756A discloses a method for preparing ursolic acid from rosemary, which is to pulverize dry rosemary leaves, add ethanol to extract 3 times, each time for 1 to 4 hours, filter and combine t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
Inventor 蒋元勇文继承雍小军朱昂辉易华李丽金
Owner 盐城市盐龙湖生态风景保护区开发建设有限公司
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