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Ultrasound-assisted extraction process of fritillariae thunbergii polysaccharide and health-care product containing fritillariae thunbergii polysaccharide

An ultrasound-assisted extraction technology of fritillary fritillary, applied in food preparation, food science, application, etc., can solve the problems of waste residue, high polysaccharide content, unrecognized polysaccharide structure and activity, etc., and achieve short extraction time and high efficiency Effect

Inactive Publication Date: 2014-01-01
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research of modern pharmacy on the active ingredients of Fritillaria is mainly aimed at small molecular compounds such as alkaloids, and the remaining waste residue is wasted in the process of extraction
However, the content of polysaccharides in Fritillaria japonicus is high, and the research on the development and utilization of polysaccharides has only seen the study of extraction technology, and there has been no research report on the structure and activity of polysaccharides, and the research on ultrasonic-assisted extraction methods has not yet been reported in the literature.

Method used

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  • Ultrasound-assisted extraction process of fritillariae thunbergii polysaccharide and health-care product containing fritillariae thunbergii polysaccharide
  • Ultrasound-assisted extraction process of fritillariae thunbergii polysaccharide and health-care product containing fritillariae thunbergii polysaccharide
  • Ultrasound-assisted extraction process of fritillariae thunbergii polysaccharide and health-care product containing fritillariae thunbergii polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1 degreasing

[0027] Clean and remove impurities from Fritillaria japonicus, accurately weigh 500g, immerse in 95% ethanol, reflux in a water bath at 80°C, take out Fritillaria japonicus samples after 3 hours, and dry them.

[0028] water extraction

[0029] Take the dried fritillaria, grind it, and extract polysaccharides according to the ultrasonic-assisted extraction process, that is, the extraction conditions are: the ratio of solid to liquid is 1:15, the ultrasonic power is 120W, the extraction temperature is 80°C, and the extraction time is 1.5h. The filter residue was repeatedly extracted three times, and the filtrates were combined to obtain an aqueous extract of Fritillaria polysaccharides.

[0030] Alcohol precipitation

[0031] Concentrate the water extract of Fritillaria polysaccharides into 500ml, add 4 times the volume of 95% ethanol solution (Fritillaria polysaccharide solution: ethanol solution = 1:4), stir while adding, and flocculent crude...

Embodiment 2

[0035] 1 degreasing

[0036] Clean up and remove impurities from Fritillaria japonicus, accurately weigh 5000g, immerse in 95% ethanol, reflux in a water bath at 80°C, take out Fritillaria japonicus samples after 3 hours, and dry them.

[0037] water extraction

[0038] Take the dried fritillaria, grind it, and extract polysaccharides according to the ultrasonic-assisted extraction process, that is, the extraction conditions are: the ratio of solid to liquid is 1:15, the ultrasonic power is 120W, the extraction temperature is 80°C, and the extraction time is 1.5h. The filter residue was repeatedly extracted three times, and the filtrates were combined to obtain an aqueous extract of Fritillaria polysaccharides.

[0039] Alcohol precipitation

[0040] Concentrate the water extract of Fritillaria polysaccharides into 1500ml, add 4 times the volume of 95% ethanol solution (Fritillaria polysaccharide solution: ethanol solution = 1:4), stir while adding, and flocculent ...

Embodiment 3

[0044] The Fritillaria polysaccharide purified by the process of the present invention is configured into a health product with a concentration ≥ 12 mg / mL, which has excellent antioxidant activity. like figure 1 It was shown that the scavenging rate of fritillaria polysaccharides to hydroxyl radicals increased with the increase of the concentration, and there was an obvious dose-effect relationship between the scavenging ability and the concentration. When the concentration is in the range of 2-8 mg / mL, the scavenging effect of polysaccharide on hydroxyl radical is significant, and when the concentration increases again, the scavenging effect of polysaccharide on hydroxyl free radical remains basically unchanged. When the concentration reaches 12mg / mL, the clearance rate is as high as 98.17%.

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Abstract

The invention discloses an ultrasound-assisted extraction process of fritillariae thunbergii polysaccharide and a health-care product containing the fritillariae thunbergii polysaccharide. The ultrasound-assisted extraction process comprises the following steps: (1) degreasing; (2) performing water extraction, crushing dried fritillariae thunbergii, extracting polysaccharide according to the ultrasound-assisted extraction process, wherein the extraction conditions are that the material-liquid ratio is 1:15, the ultrasonic action power is 120W, the extraction temperature is 80 DEG C, and the extraction time is 1.5 h, repeatedly extracting filter residues for at least three times, and combining filtrates to obtain fritillariae thunbergii polysaccharide water extract; (3) performing alcohol precipitation; (4) removing protein to obtain purified fritillariae thunbergii polysaccharide. The ultrasound-assisted extraction process has the advantages of short extraction time and high efficiency; the health-care product containing the fritillariae thunbergii polysaccharide has the health-care effects of elimination of free radicals and strong oxidation resistance.

Description

technical field [0001] The invention belongs to the field of compound extraction, and specifically refers to an ultrasonic-assisted extraction process of fritillary fritillaria polysaccharides. Background technique [0002] As a natural polymer compound from higher plants, animals and microbial cells, polysaccharides are a type of complex and large sugar substances formed by the condensation and dehydration of multiple monosaccharide molecules. They are the four basic elements of life. one of the substances. Polysaccharides have been continuously excavated due to their important physiological functions and wide applications. Polysaccharides as medicines began in 1943. At present, people have successfully extracted polysaccharides from many biological materials. These polysaccharides have very important and special physiological activities. [0003] Fritillaria thunbergiiMiq. is a perennial herb of the genus Fritillaria in the family Liliaceae. Underground bulbs have the fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L1/09A23L33/125
Inventor 郑赛张旭周丹楠叶美良郑志飞吴明江
Owner WENZHOU UNIVERSITY