Set of primers and probe used for real-time fluorescence PCR detection of neisseria gonorrhoeae and applications of set of primers and probe

Abstract

The invention discloses a set of primers and a probe used for real-time fluorescence PCR detection of neisseria gonorrhoeae, and more specifically discloses nucleotide sequences of an upstream primer NG16s169F, a downstream primer NG16s235R, and the probe NG16s210P. Advantages of the set of primers and the probe used for real-time fluorescence PCR detection of neisseria gonorrhoeae include that: sensitivity is high, specificity is excellent, operation is convenient, and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a set of primers and probes for real-time fluorescent PCR detection of Neisseria gonorrhoeae and applications thereof. Background technique [0002] Neisseria gonorrhoeae ( Neisseria gonorrhoeae , NG) is a common pathogen of urogenital-transmitted diseases. The clinical manifestations caused by gonorrhea infection depend on the degree of infection, the sensitivity of the body, the virulence of the bacteria, the site of infection and the duration of infection, and the health of the body. Its incubation period is short and its infectivity is strong. The most common is symptomatic infection of the genitourinary system, such as urethritis, cervicitis, epididymitis, prostatitis, salpingitis, etc. If not treated in time, gonorrhea can invade the eyes, The body, skin, rectum, pelvic cavity and other parts can even spread to other parts of the body through blood, forming go...

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Problems solved by technology

Fluorescent dye intercalation technology uses SYBR green I to intercalate DNA double strands to increase the fluorescence intensity. The fluorescent signal is introduced into double stranded DNA. This method has poor specificity, and the results are often interfered by the presence of primer dimers, resulting in false positives. and other problems, so it is not suitable for clinical use. At present, there is a patent for the method of detecting Neisseria gonorrhoeae using self-quenching fluorescence technology in China, and the patent number is 200510020234.3. cppB gene GenBank accession number L40552) as a template, recent studies have shown that the cppB gene has cross-reactivity in some Neisseria gonorrhoeae strains and the target sequence is lacking in some Neisseria gonorrhoeae strains. increased target gene

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