Method for rapidly separating lentinan

A fast technology of lentinan, applied in the field of separation and purification of biomacromolecules, to achieve rapid separation, optimize separation conditions, and reduce production costs

Active Publication Date: 2014-01-15
东台海滨科技创业园管理有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there are no research literature or patents on the separation of active polysaccharides using the ionic liquid two-phase system formed by ionic liquid [Bmim]Cl and inorganic phosphate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Extraction of crude polysaccharides from mushrooms

[0016] Crush and sieve the fruiting bodies of shiitake mushrooms, reflux petroleum ether in a constant temperature water bath at 45°C for 6 h, degrease for 3 times, and dry naturally; weigh 100 g of defatted shiitake mushroom powder and add 2000 mL of water to dissolve, extract with hot water at 95°C for 6-8 h, repeat the operation 3 times, collect the supernatant by centrifugation, combine, concentrate, precipitate with 4 times the volume of 95% ethanol, stand overnight at 4°C, collect the precipitate by centrifugation, and freeze-dry to obtain the crude polysaccharide of Lentinus edodes. The measured polysaccharide content was 53.7%.

Embodiment 2

[0017] Example 2: Separation of lentinan by ionic liquid two-phase system

[0018] Add 1.0 g ionic liquid [Bmim]Cl, 2.0 g K to a graduated centrifuge tube 3 PO 4 , 4.0 mL of distilled water, 1 mL of Lentinus edodes crude polysaccharide solution with a concentration of 5 mg / mL was shaken sufficiently to completely dissolve the ionic liquid and inorganic salts, and stood in a constant temperature water bath at 50°C for 30 min to separate the upper and lower phases. The lower inorganic salt phase was dialyzed with distilled water for 3 days, concentrated and freeze-dried to obtain lentinan. The upper ionic liquid phase was concentrated (≤45°C), extracted three times with dichloromethane, the dichloromethane phase was concentrated (≤45°C), and the ionic liquid [Bmim]Cl was recovered for reuse. The extraction rate of Lentinan was measured to be 83.7%, and the polysaccharide content was 94.3%.

Embodiment 3

[0020] The test treatment process is the same as in Example 2, except that the concentration of the crude polysaccharide of Lentinus edodes is 15 mg / mL, K 3 PO 4 With a mass of 2.5 g and a constant temperature water bath at 25°C, the extraction rate of lentinan was 91.8%, and the polysaccharide content was 93.5%.

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Abstract

The invention relates to a method for rapidly separating lentinan and belongs to the technical field of biomacromolecule separation and purification, in particular to a method for rapidly separating lentinan using an ionic liquid two-aqueous phase system composed of [Bmim]Cl/K3PO4. The method is green, highly-efficient and rapid, is suitable for large-scale industrial production of active polysaccharides, reduces the production cost by recycling the ionic liquid, and can be widely applied to the separation and purification of fungal polysaccharides.

Description

technical field [0001] The present invention relates to a method for quick separation of lentinan, in particular to ionic liquid [Bmim]Cl / inorganic phosphate K 3 PO 4 The invention relates to a method for rapidly separating lentinan by an ionic liquid two-phase aqueous system, and belongs to the technical field of separation and purification of biological macromolecules. Background technique [0002] mushroom( Lentinula edodes ), also known as shiitake mushrooms and winter mushrooms, are fungi of the Basidiomycetes Apiaceae family. Anti-inflammatory and other functions. Although lentinan has clear pharmacological activity, it must be of sufficient purity to be used clinically, especially as an injection drug. The separation and purification technology of lentinan is in a leading position in the United States and Japan, and is protected by patents. The polysaccharides produced by most domestic lentinan manufacturers are products with low purity, and generally can only be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 闫景坤马海乐王振斌裴娟娟
Owner 东台海滨科技创业园管理有限公司
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