Method for rapidly separating lentinan
A fast technology of lentinan, applied in the field of separation and purification of biomacromolecules, to achieve rapid separation, optimize separation conditions, and reduce production costs
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Embodiment 1
[0015] Embodiment 1: Extraction of crude polysaccharides from mushrooms
[0016] Crush and sieve the fruiting bodies of shiitake mushrooms, reflux petroleum ether in a constant temperature water bath at 45°C for 6 h, degrease for 3 times, and dry naturally; weigh 100 g of defatted shiitake mushroom powder and add 2000 mL of water to dissolve, extract with hot water at 95°C for 6-8 h, repeat the operation 3 times, collect the supernatant by centrifugation, combine, concentrate, precipitate with 4 times the volume of 95% ethanol, stand overnight at 4°C, collect the precipitate by centrifugation, and freeze-dry to obtain the crude polysaccharide of Lentinus edodes. The measured polysaccharide content was 53.7%.
Embodiment 2
[0017] Example 2: Separation of lentinan by ionic liquid two-phase system
[0018] Add 1.0 g ionic liquid [Bmim]Cl, 2.0 g K to a graduated centrifuge tube 3 PO 4 , 4.0 mL of distilled water, 1 mL of Lentinus edodes crude polysaccharide solution with a concentration of 5 mg / mL was shaken sufficiently to completely dissolve the ionic liquid and inorganic salts, and stood in a constant temperature water bath at 50°C for 30 min to separate the upper and lower phases. The lower inorganic salt phase was dialyzed with distilled water for 3 days, concentrated and freeze-dried to obtain lentinan. The upper ionic liquid phase was concentrated (≤45°C), extracted three times with dichloromethane, the dichloromethane phase was concentrated (≤45°C), and the ionic liquid [Bmim]Cl was recovered for reuse. The extraction rate of Lentinan was measured to be 83.7%, and the polysaccharide content was 94.3%.
Embodiment 3
[0020] The test treatment process is the same as in Example 2, except that the concentration of the crude polysaccharide of Lentinus edodes is 15 mg / mL, K 3 PO 4 With a mass of 2.5 g and a constant temperature water bath at 25°C, the extraction rate of lentinan was 91.8%, and the polysaccharide content was 93.5%.
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